Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4252043..4252540 Replicon chromosome
Accession NZ_CP017979
Organism Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag BL257_RS22455 Protein ID WP_001173343.1
Coordinates 4252367..4252540 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag BL257_RS22450 Protein ID WP_000398619.1
Coordinates 4252043..4252339 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BL257_RS22405 (BL257_20985) 4247438..4248157 - 720 WP_000611288.1 two-component system response regulator DcuR -
BL257_RS22410 (BL257_20990) 4248154..4248510 - 357 Protein_4010 GHKL domain-containing protein -
BL257_RS22430 (BL257_21005) 4249841..4251121 - 1281 Protein_4012 DcuS/MalK family sensor histidine kinase -
BL257_RS22435 (BL257_21010) 4251302..4251532 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
BL257_RS22440 (BL257_21015) 4251544..4251816 + 273 WP_000405651.1 N-acetyltransferase -
BL257_RS22450 (BL257_21020) 4252043..4252339 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
BL257_RS22455 (BL257_21025) 4252367..4252540 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
BL257_RS22460 (BL257_21030) 4252659..4254176 - 1518 WP_001295074.1 lysine--tRNA ligase -
BL257_RS22465 (BL257_21035) 4254413..4255870 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4248154..4261393 13239


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T68443 WP_001173343.1 NZ_CP017979:4252367-4252540 [Escherichia coli str. K-12 substr. W3110]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T68443 NZ_CP017979:4252367-4252540 [Escherichia coli str. K-12 substr. W3110]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT68443 WP_000398619.1 NZ_CP017979:4252043-4252339 [Escherichia coli str. K-12 substr. W3110]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT68443 NZ_CP017979:4252043-4252339 [Escherichia coli str. K-12 substr. W3110]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References