257217

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 445660..446157 Replicon chromosome
Accession NZ_CP104114
Organism Escherichia coli strain USDA-ARS-USMARC-51408

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N1706_RS02040 Protein ID WP_001173343.1
Coordinates 445984..446157 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N1706_RS02035 Protein ID WP_000398619.1
Coordinates 445660..445956 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1706_RS02015 (442391) 442391..443110 - 720 WP_000611288.1 two-component system response regulator DcuR -
N1706_RS02020 (443107) 443107..444738 - 1632 WP_001216477.1 sensor histidine kinase -
N1706_RS02025 (444919) 444919..445149 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
N1706_RS02030 (445161) 445161..445433 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
N1706_RS02035 (445660) 445660..445956 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N1706_RS02040 (445984) 445984..446157 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N1706_RS02045 (446276) 446276..447793 - 1518 WP_001295074.1 lysine--tRNA ligase -
N1706_RS02050 (448030) 448030..449487 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 445660..455010 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T257217 WP_001173343.1 NZ_CP104114:445984-446157 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT257217 WP_000398619.1 NZ_CP104114:445660-445956 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References