Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4295792..4296289 Replicon chromosome
Accession NZ_LM993812
Organism Escherichia coli strain K-12 substr. HMS174

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECHMS174_RS21150 Protein ID WP_001173343.1
Coordinates 4296116..4296289 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECHMS174_RS21145 Protein ID WP_000398619.1
Coordinates 4295792..4296088 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECHMS174_RS21125 4292523..4293242 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECHMS174_RS21130 4293239..4294870 - 1632 WP_001216477.1 sensor histidine kinase -
ECHMS174_RS21135 4295051..4295281 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
ECHMS174_RS21140 4295293..4295565 + 273 WP_000405651.1 N-acetyltransferase -
ECHMS174_RS21145 4295792..4296088 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECHMS174_RS21150 4296116..4296289 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECHMS174_RS21155 4296408..4297925 - 1518 WP_001295074.1 lysine--tRNA ligase -
ECHMS174_RS21160 4298162..4299619 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4295792..4305142 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T285413 WP_001173343.1 NZ_LM993812:4296116-4296289 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT285413 WP_000398619.1 NZ_LM993812:4295792-4296088 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References