201001

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3392987..3393484 Replicon chromosome
Accession NZ_CP073996
Organism Escherichia coli strain PM7

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFU86_RS16420 Protein ID WP_001173343.1
Coordinates 3393311..3393484 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KFU86_RS16415 Protein ID WP_000398619.1
Coordinates 3392987..3393283 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU86_RS16395 (3389718) 3389718..3390437 - 720 WP_000611288.1 two-component system response regulator DcuR -
KFU86_RS16400 (3390434) 3390434..3392065 - 1632 WP_001216477.1 sensor histidine kinase -
KFU86_RS16405 (3392246) 3392246..3392476 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KFU86_RS16410 (3392488) 3392488..3392760 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
KFU86_RS16415 (3392987) 3392987..3393283 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFU86_RS16420 (3393311) 3393311..3393484 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFU86_RS16425 (3393603) 3393603..3395120 - 1518 WP_001295074.1 lysine--tRNA ligase -
KFU86_RS16430 (3395357) 3395357..3396814 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3392987..3402337 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T201001 WP_001173343.1 NZ_CP073996:3393311-3393484 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T201001 NZ_CP098206:4167435-4167587 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT201001 WP_000398619.1 NZ_CP073996:3392987-3393283 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT201001 NZ_CP098206:c4167386-4167329 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References