Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4429443..4429940 Replicon chromosome
Accession NZ_CP008801
Organism Escherichia coli KLY

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HQ24_RS21885 Protein ID WP_001173343.1
Coordinates 4429767..4429940 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HQ24_RS21880 Protein ID WP_000398619.1
Coordinates 4429443..4429739 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQ24_RS21860 4426174..4426893 - 720 WP_000611288.1 two-component system response regulator DcuR -
HQ24_RS21865 4426890..4428521 - 1632 WP_001216477.1 sensor histidine kinase -
HQ24_RS21870 4428702..4428932 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
HQ24_RS21875 4428944..4429216 + 273 WP_000405651.1 N-acetyltransferase -
HQ24_RS21880 4429443..4429739 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HQ24_RS21885 4429767..4429940 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HQ24_RS21890 4430059..4431576 - 1518 WP_001295074.1 lysine--tRNA ligase -
HQ24_RS21895 4431813..4433270 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4429443..4438793 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T47529 WP_001173343.1 NZ_CP008801:4429767-4429940 [Escherichia coli KLY]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T47529 NZ_CP008801:4429767-4429940 [Escherichia coli KLY]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT47529 WP_000398619.1 NZ_CP008801:4429443-4429739 [Escherichia coli KLY]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT47529 NZ_CP008801:4429443-4429739 [Escherichia coli KLY]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References