Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4404375..4404872 Replicon chromosome
Accession NZ_CP054665
Organism Escherichia coli strain IGC_EcoliInv_1.1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUR94_RS21500 Protein ID WP_001173343.1
Coordinates 4404699..4404872 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUR94_RS21495 Protein ID WP_000398619.1
Coordinates 4404375..4404671 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUR94_RS21475 (4401106) 4401106..4401825 - 720 WP_000611288.1 two-component system response regulator DcuR -
HUR94_RS21480 (4401822) 4401822..4403453 - 1632 WP_001216477.1 sensor histidine kinase -
HUR94_RS21485 (4403634) 4403634..4403864 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
HUR94_RS21490 (4403876) 4403876..4404148 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
HUR94_RS21495 (4404375) 4404375..4404671 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUR94_RS21500 (4404699) 4404699..4404872 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUR94_RS21505 (4404991) 4404991..4406508 - 1518 WP_001295074.1 lysine--tRNA ligase -
HUR94_RS21510 (4406745) 4406745..4408202 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4404375..4413725 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T160066 WP_001173343.1 NZ_CP054665:4404699-4404872 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T160066 NZ_CP070287:3527593-3527811 [Escherichia albertii]
ATGTCCGATAAACCTTTAACAAAAACCGATTATTTGATGCGTCTGCGTCGTTGCCAGACAATTGACACGCTGGAACGTGT
TATCGAGAAAAATAAATATGAATTATCAGATAATGAATTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTTGCAGAGC
TGACTATGAATAAGCTATATGACAAGATCCCTTCCTCAGTGTGGAAATTCATTCGGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT160066 WP_000398619.1 NZ_CP054665:4404375-4404671 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT160066 NZ_CP070287:3527193-3527567 [Escherichia albertii]
ATGGATGAATACTCACCAAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGTAACCATGGCTGGGTAAATGACCCAACCTCGGCAATCAATCTACAGCTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAACAGATTGATGAATATCTG
GACGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAACCGATT
GTTCCGTTGCTTTGTTAACGCCACCAAAGCGAATCCTGCAAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References