253589

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4229976..4230473 Replicon chromosome
Accession NZ_CP102380
Organism Escherichia coli strain BM28

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NR349_RS20455 Protein ID WP_001173343.1
Coordinates 4230300..4230473 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NR349_RS20450 Protein ID WP_000398619.1
Coordinates 4229976..4230272 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NR349_RS20430 (4226708) 4226708..4227427 - 720 WP_000611288.1 two-component system response regulator DcuR -
NR349_RS20435 (4227424) 4227424..4229055 - 1632 WP_001216477.1 sensor histidine kinase -
NR349_RS20440 (4229236) 4229236..4229465 + 230 Protein_3974 (4Fe-4S)-binding protein -
NR349_RS20445 (4229477) 4229477..4229749 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
NR349_RS20450 (4229976) 4229976..4230272 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NR349_RS20455 (4230300) 4230300..4230473 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NR349_RS20460 (4230592) 4230592..4232109 - 1518 WP_001295074.1 lysine--tRNA ligase -
NR349_RS20465 (4232346) 4232346..4233803 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4229976..4239326 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T253589 WP_001173343.1 NZ_CP102380:4230300-4230473 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT253589 WP_000398619.1 NZ_CP102380:4229976-4230272 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References