Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type I Classification (family/domain) CD-RCd/-
Location 1124125..1124339 Replicon chromosome
Accession NC_009089
Organism Clostridium difficile 630
T1TAdb ID TA03822

Toxin (Protein)


Gene name CD0956.2 Uniprot ID Q183Z5
Locus tag CD630_RS19695 Protein ID WP_003429855.1
Coordinates 1124125..1124286 (+) Length 54 a.a.

Antitoxin (RNA)


Gene name RCd10
Locus tag -
Coordinates 1124206..1124339 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CD630_RS05340 (CD630_09490) 1119464..1119697 + 234 WP_009896624.1 hypothetical protein -
CD630_RS05345 (CD630_09500) 1119707..1120108 + 402 WP_009888836.1 hypothetical protein -
CD630_RS05350 (CD630_09510) 1120102..1120449 + 348 WP_003429844.1 hypothetical protein -
CD630_RS05355 (CD630_09520) 1120449..1120874 + 426 WP_011861030.1 HK97 gp10 family phage protein -
CD630_RS05360 (CD630_09530) 1120867..1121304 + 438 WP_009901504.1 hypothetical protein -
CD630_RS19690 (CD630_09531) 1121297..1121473 + 177 WP_011861031.1 hypothetical protein -
CD630_RS05365 (CD630_09540) 1121475..1122785 + 1311 WP_011861032.1 phage tail sheath family protein -
CD630_RS05370 (CD630_09550) 1122802..1123272 + 471 WP_003429851.1 phage tail tube protein -
CD630_RS05375 (CD630_09560) 1123344..1123784 + 441 WP_003429853.1 phage portal protein -
CD630_RS19695 (CD630_09562) 1124125..1124286 + 162 WP_003429855.1 hypothetical protein Toxin
- 1124206..1124339 - 134 - - Antitoxin
CD630_RS05385 (CD630_09570) 1125950..1126510 + 561 WP_011861034.1 lipoprotein -
CD630_RS05390 (CD630_09580) 1126554..1127123 + 570 WP_011861035.1 zinc-ribbon domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1078053..1143688 65635


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.



Sequences

Toxin        


Download         Length: 54 a.a.        Molecular weight: 6120.42 Da        Isoelectric Point: 10.8938

>T10173 WP_003429855.1 NC_009089:1124125-1124286 [Clostridioides difficile 630]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Download         Length: 162 bp

>T10173 NC_009089:1124125-1124286 [Clostridioides difficile 630]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin


Download         Length: 134 bp

>AT10173 NC_009089:c1124339-1124206 [Clostridioides difficile 630]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10172 Clostridium difficile 630

100

100

1
T10174 Clostridium difficile 630

52.83

100

0.56
T10176 Clostridium difficile 630

50.943

100

0.54
T10081 Clostridium difficile 630

49.02

100

0.532
T10080 Clostridium difficile 630

47.059

100

0.511
T10171 Clostridium difficile 630

49.057

100

0.5
T10175 Clostridium difficile 630

53.333

90

0.48
T10084 Clostridium difficile 630

35.135

100

0.382
T10083 Clostridium difficile 630

35.135

100

0.382
T10082 Clostridium difficile 630

32.353

97.143

0.314

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A069AVN2

Antitoxin

Download structure file

References

(1) Anna Maikova et al. (2018) Discovery of new type I toxin-antitoxin systems adjacent to CRISPR arrays in Clostridium difficile. Nucleic Acids Research 46(9):4733-4751. [PubMed:29529286] experimental


experimental experimental literature