Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | CD-RCd/- |
| Location | 1089560..1089726 | Replicon | chromosome |
| Accession | NC_009089 | ||
| Organism | Clostridium difficile 630 | ||
| T1TAdb ID | TA07132 | ||
Toxin (Protein)
| Gene name | CD0904.1 | Uniprot ID | - |
| Locus tag | - | Protein ID | - |
| Coordinates | 1089560..1089667 (+) | Length | 36 a.a. |
Antitoxin (RNA)
| Gene name | RCd13 | ||
| Locus tag | - | ||
| Coordinates | 1089620..1089726 (-) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CD630_RS05095 (CD630_09020) | 1086376..1087242 | + | 867 | WP_003437125.1 | cation diffusion facilitator family transporter | - |
| CD630_RS05100 | 1087383..1088021 | + | 639 | Protein_924 | aldo/keto reductase | - |
| CD630_RS05105 (CD630_09040) | 1088175..1089293 | - | 1119 | WP_011860983.1 | site-specific integrase | - |
| - | 1089560..1089667 | + | 108 | - | - | Toxin |
| - | 1089620..1089726 | - | 107 | - | - | Antitoxin |
| CD630_RS05110 (CD630_09050) | 1089801..1091465 | - | 1665 | WP_011860984.1 | DUF4041 domain-containing protein | - |
| CD630_RS05115 (CD630_09060) | 1091736..1093184 | - | 1449 | WP_011860985.1 | SAP domain-containing protein | - |
| CD630_RS05120 (CD630_09070) | 1093832..1094164 | - | 333 | WP_011860986.1 | helix-turn-helix transcriptional regulator | - |
| CD630_RS05125 (CD630_09071) | 1094326..1094514 | + | 189 | WP_011860987.1 | helix-turn-helix transcriptional regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Prophage | - | - | 1078053..1143688 | 65635 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 36 a.a. Molecular weight: 3916.68 Da Isoelectric Point: 10.1605
>T10082 - NC_009089:1089560-1089667 [Clostridioides difficile 630]
MLINFLLSIVAGIVSAYIYEKIKNHPNANKGDLKK
MLINFLLSIVAGIVSAYIYEKIKNHPNANKGDLKK
Download Length: 108 bp
>T10082 NC_009089:1089560-1089667 [Clostridioides difficile 630]
ATGTTAATTAACTTTTTATTGAGTATAGTAGCTGGAATTGTGTCAGCTTACATTTACGAGAAAATAAAAAATCACCCAAA
CGCCAATAAGGGTGATTTAAAGAAATAA
ATGTTAATTAACTTTTTATTGAGTATAGTAGCTGGAATTGTGTCAGCTTACATTTACGAGAAAATAAAAAATCACCCAAA
CGCCAATAAGGGTGATTTAAAGAAATAA
Antitoxin
Download Length: 107 bp
>AT10082 NC_009089:c1089726-1089620 [Clostridioides difficile 630]
AAGGAGATAAAATTTACTTTGTAAAAGGTGAATTTCTCTAATTGATTAGAATTTAAAAGTTATTTCTTTAAATCACCCTT
ATTGGCGTTTGGGTGATTTTTTATTTT
AAGGAGATAAAATTTACTTTGTAAAAGGTGAATTTCTCTAATTGATTAGAATTTAAAAGTTATTTCTTTAAATCACCCTT
ATTGGCGTTTGGGTGATTTTTTATTTT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10084 | Clostridium difficile 630 |
79.412 |
100 |
0.794 |
| T10083 | Clostridium difficile 630 |
79.412 |
100 |
0.794 |
| T10176 | Clostridium difficile 630 |
36.842 |
100 |
0.4 |
| T10172 | Clostridium difficile 630 |
32.353 |
97.143 |
0.314 |
| T10173 | Clostridium difficile 630 |
32.353 |
97.143 |
0.314 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Johann Peltier et al. (2020) Type I toxin-antitoxin systems contribute to the maintenance of mobile genetic elements in Clostridioides difficile. Communications Biology 3(1):718. [PubMed:33247281]