Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | CD-RCd/- |
| Location | 1644762..1644958 | Replicon | chromosome |
| Accession | NC_009089 | ||
| Organism | Clostridium difficile 630 | ||
| T1TAdb ID | TA03824 | ||
Toxin (Protein)
| Gene name | CD1418.2 | Uniprot ID | Q18BT8 |
| Locus tag | CD630_RS19720 | Protein ID | WP_009902496.1 |
| Coordinates | 1644762..1644914 (+) | Length | 51 a.a. |
Antitoxin (RNA)
| Gene name | CD630_n00500 | ||
| Locus tag | - | ||
| Coordinates | 1644858..1644958 (-) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CD630_RS07800 (CD630_14150) | 1640302..1640895 | - | 594 | WP_003438546.1 | DUF308 domain-containing protein | - |
| CD630_RS07805 (CD630_14160) | 1641002..1641430 | + | 429 | WP_011861207.1 | DMT family transporter | - |
| CD630_RS07810 (CD630_14170) | 1641497..1642165 | + | 669 | WP_003429358.1 | diphthine--ammonia ligase | - |
| CD630_RS07815 (CD630_14180) | 1642283..1643638 | - | 1356 | WP_003438549.1 | MATE family efflux transporter | - |
| CD630_RS19720 (CD630_14182) | 1644762..1644914 | + | 153 | WP_009902496.1 | hypothetical protein | Toxin |
| - | 1644858..1644958 | - | 101 | - | - | Antitoxin |
| CD630_RS07820 (CD630_14190) | 1646344..1648077 | - | 1734 | WP_009902497.1 | diguanylate cyclase | - |
| CD630_RS07825 (CD630_14200) | 1648317..1649162 | - | 846 | WP_009889222.1 | GGDEF domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 51 a.a. Molecular weight: 5742.81 Da Isoelectric Point: 10.9178
>T10174 WP_009902496.1 NC_009089:1644762-1644914 [Clostridioides difficile 630]
MDNFLQGVLASLVASLIVYLNSKLFKKVKSHSGRSDFSFELKIKFKKNKH
MDNFLQGVLASLVASLIVYLNSKLFKKVKSHSGRSDFSFELKIKFKKNKH
Download Length: 153 bp
>T10174 NC_009089:1644762-1644914 [Clostridioides difficile 630]
ATGGATAATTTTTTACAAGGTGTACTAGCAAGTTTAGTTGCCAGTTTAATAGTTTACTTAAATAGTAAATTGTTTAAAAA
AGTAAAAAGCCACTCTGGCAGGAGTGACTTTAGTTTTGAACTAAAAATCAAGTTCAAAAAGAATAAACATTAG
ATGGATAATTTTTTACAAGGTGTACTAGCAAGTTTAGTTGCCAGTTTAATAGTTTACTTAAATAGTAAATTGTTTAAAAA
AGTAAAAAGCCACTCTGGCAGGAGTGACTTTAGTTTTGAACTAAAAATCAAGTTCAAAAAGAATAAACATTAG
Antitoxin
Download Length: 101 bp
>AT10174 NC_009089:c1644958-1644858 [Clostridioides difficile 630]
AAGAAGAACTACAATCTATTTAGACGTAGAGTGAAGTTCAAATACTAATGTTTATTCTTTTTGAACTTGATTTTTAGTTC
AAAACTAAAGTCACTCCTGCC
AAGAAGAACTACAATCTATTTAGACGTAGAGTGAAGTTCAAATACTAATGTTTATTCTTTTTGAACTTGATTTTTAGTTC
AAAACTAAAGTCACTCCTGCC
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10081 | Clostridium difficile 630 |
62 |
100 |
0.66 |
| T10175 | Clostridium difficile 630 |
64 |
100 |
0.64 |
| T10080 | Clostridium difficile 630 |
60 |
100 |
0.638 |
| T10171 | Clostridium difficile 630 |
59.615 |
100 |
0.62 |
| T10176 | Clostridium difficile 630 |
58 |
100 |
0.58 |
| T10173 | Clostridium difficile 630 |
52.83 |
100 |
0.56 |
| T10172 | Clostridium difficile 630 |
52.83 |
100 |
0.56 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Anna Maikova et al. (2018) Discovery of new type I toxin-antitoxin systems adjacent to CRISPR arrays in Clostridium difficile. Nucleic Acids Research 46(9):4733-4751. [PubMed:29529286]