Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | CD-RCd/- |
| Location | 2908124..2908333 | Replicon | chromosome |
| Accession | NC_009089 | ||
| Organism | Clostridium difficile 630 | ||
| T1TAdb ID | TA03826 | ||
Toxin (Protein)
| Gene name | CD2517.1 | Uniprot ID | Q182K5 |
| Locus tag | CD630_RS19820 | Protein ID | WP_004454815.1 |
| Coordinates | 2908175..2908333 (-) | Length | 53 a.a. |
Antitoxin (RNA)
| Gene name | RCd8 | ||
| Locus tag | - | ||
| Coordinates | 2908124..2908258 (+) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CD630_RS13500 (CD630_25150) | 2903694..2905322 | + | 1629 | WP_004454813.1 | aspartate 4-decarboxylase | - |
| CD630_RS13505 (CD630_25160) | 2905443..2906438 | + | 996 | WP_003431031.1 | asparaginase | - |
| CD630_RS13510 (CD630_25170) | 2906616..2906999 | - | 384 | WP_011861577.1 | BlaI/MecI/CopY family transcriptional regulator | - |
| - | 2908124..2908258 | + | 135 | - | - | Antitoxin |
| CD630_RS19820 (CD630_25171) | 2908175..2908333 | - | 159 | WP_004454815.1 | hypothetical protein | Toxin |
| CD630_RS13515 (CD630_25180) | 2908692..2910062 | - | 1371 | WP_011861578.1 | cell wall-binding protein Cwp29 | - |
| CD630_RS13520 (CD630_25190) | 2910276..2911394 | + | 1119 | WP_011861579.1 | site-specific integrase | - |
| CD630_RS13525 (CD630_25200) | 2911711..2912379 | - | 669 | WP_011861580.1 | VanZ family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 53 a.a. Molecular weight: 5996.20 Da Isoelectric Point: 11.3102
>T10171 WP_004454815.1 NC_009089:c2908333-2908175 [Clostridioides difficile 630]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK
Download Length: 159 bp
>T10171 NC_009089:c2908333-2908175 [Clostridioides difficile 630]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA
Antitoxin
Download Length: 135 bp
>AT10171 NC_009089:2908124-2908258 [Clostridioides difficile 630]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10174 | Clostridium difficile 630 |
59.615 |
100 |
0.62 |
| T10173 | Clostridium difficile 630 |
49.057 |
100 |
0.5 |
| T10172 | Clostridium difficile 630 |
49.057 |
100 |
0.5 |
| T10175 | Clostridium difficile 630 |
52.083 |
96 |
0.5 |
| T10080 | Clostridium difficile 630 |
46 |
100 |
0.489 |
| T10081 | Clostridium difficile 630 |
42.308 |
100 |
0.468 |
| T10176 | Clostridium difficile 630 |
44.231 |
100 |
0.46 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Anna Maikova et al. (2018) Discovery of new type I toxin-antitoxin systems adjacent to CRISPR arrays in Clostridium difficile. Nucleic Acids Research 46(9):4733-4751. [PubMed:29529286]