Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS01208  |
| T6SS type | Type i1 |
| Strain | Aeromonas veronii strain TH0426 |
| Replicon (RefSeq) | chromosome (NZ_CP012504) |
| Location | 2455395..2478287 |
| Function | anti-eukaryotic |
| Comment | The toxicity of the wild-type strain TH0426 to EPC cells was 1.8-fold higher than that of the deletion strain ΔdotU at 1h of infection. At 2h, the cytotoxicity reached a maximum of 2.1-fold more than that of the mutant strain, the virulence was reduced significantly. |
| References | [1] Song H, Kang Y, Qian A, Shan X, Li Y, Zhang L, Zhang H, Sun W. et al (2020) Inactivation of the T6SS inner membrane protein DotU results in severe attenuation and decreased pathogenicity of Aeromonas veronii TH0426.. BMC Microbiol. 20(1):76. [PudMed:32245412] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TagH | TssA | TssB | TssC | TssE | TssF | TssH | TssJ | TssK | TssL | TssM |
| Number | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| AMS64_RS11245 (rstB) | 2450415..2451740 | + | 1326 | WP_064336271.1 | two-component system sensor histidine kinase RstB | ||
| AMS64_RS11250 | 2451795..2451977 | + | 183 | WP_005337116.1 | hypothetical protein | ||
| AMS64_RS11255 (mprF) | 2452131..2454680 | + | 2550 | WP_064336272.1 | bifunctional lysylphosphatidylglycerol flippase/synthetase MprF | ||
| AMS64_RS11260 | 2454692..2455369 | + | 678 | pseudogene | virulence factor | ||
| AMS64_RS11265 | 2455395..2457305 | - | 1911 | pseudogene | type VI secretion system tip protein VgrG | ||
| AMS64_RS11270 | 2457317..2457604 | - | 288 | WP_064336273.1 | type VI secretion system PAAR protein | ||
| T6CP016030 | TssA | AMS64_RS11275 | 2457910..2459337 | - | 1428 | WP_064336274.1 | type VI secretion system ImpA family N-terminal domain-containing protein |
| T6CP016031 | TssM | AMS64_RS11280 (tssM) | 2459381..2462878 | - | 3498 | WP_064336275.1 | type VI secretion system membrane subunit TssM |
| T6CP016032 | TssA | AMS64_RS11285 (tssA) | 2462920..2464347 | - | 1428 | WP_064336276.1 | type VI secretion system protein TssA |
| AMS64_RS11290 (tagO) | 2464356..2464961 | - | 606 | WP_064336277.1 | type VI secretion system-associated protein TagO | ||
| AMS64_RS11295 | 2464961..2466499 | - | 1539 | WP_019444941.1 | sigma-54 dependent transcriptional regulator | ||
| T6CP016033 | TssH | AMS64_RS11300 (tssH) | 2466502..2469144 | - | 2643 | WP_064336278.1 | type VI secretion system ATPase TssH |
| T6CP016034 | TssL | AMS64_RS11305 | 2469165..2469944 | - | 780 | WP_019444943.1 | type IVB secretion system protein IcmH/DotU |
| T6CP016035 | TssK | AMS64_RS11310 (tssK) | 2469968..2471302 | - | 1335 | WP_064336279.1 | type VI secretion system baseplate subunit TssK |
| T6CP016036 | TssJ | AMS64_RS11315 (tssJ) | 2471305..2471820 | - | 516 | WP_064336280.1 | type VI secretion system lipoprotein TssJ |
| T6CP016037 | TagH | AMS64_RS11320 (tagH) | 2471820..2473055 | - | 1236 | WP_064336281.1 | type VI secretion system-associated FHA domain protein TagH |
| AMS64_RS11325 (tssG) | 2473095..2474093 | - | 999 | pseudogene | type VI secretion system baseplate subunit TssG | ||
| T6CP016038 | TssF | AMS64_RS11330 (tssF) | 2474057..2475823 | - | 1767 | WP_019444948.1 | type VI secretion system baseplate subunit TssF |
| T6CP016039 | TssE | AMS64_RS11335 (tssE) | 2475827..2476258 | - | 432 | WP_005299981.1 | type VI secretion system baseplate subunit TssE |
| T6CP016040 | TssC | AMS64_RS11340 (tssC) | 2476265..2477746 | - | 1482 | WP_064336282.1 | type VI secretion system contractile sheath large subunit |
| T6CP016041 | TssB | AMS64_RS11345 (tssB) | 2477784..2478287 | - | 504 | WP_021230530.1 | type VI secretion system contractile sheath small subunit |
| AMS64_RS11355 | 2480022..2480477 | - | 456 | WP_005337119.1 | YbjN domain-containing protein | ||
| AMS64_RS11360 | 2480636..2481973 | + | 1338 | WP_064336283.1 | flavohemoglobin expression-modulating QEGLA motif protein | ||
| AMS64_RS23045 | 2482045..2483264 | - | 1220 | pseudogene | IS3 family transposase |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
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Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
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Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
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Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
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