SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016036
Component protein type	TssJ
T6SS ID (Type)	T6SS01208 (Type i1)
Strain	Aeromonas veronii strain TH0426
Replicon	chromosome
Sequence	Protein sequence (172 a.a.); Nucleotide sequence (516 bp)
Reference	[1] Song H, Kang Y, Qian A, et al. Inactivation of the T6SS inner membrane protein DotU results in severe attenuation and decreased pathogenicity of Aeromonas veronii TH0426. BMC Microbiol. 2020 Apr 3;20(1):76. doi: 10.1186/s12866-020-01743-5. PMID: 32245412

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AMS64_RS11315
Coordinate (Strand)	2471305..2471820 (-)
NCBI ID	WP_064336280.1
RefSeq	NZ_CP012504
Uniprot ID	A0A2S3XL12_9GAMM
KEGG ID	avo:AMS64_11295
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	4.3e-33	43..160

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-171	MIRALMLGAALLTLAGCTTMGKMADVAMNPDIQVGSNDDQPSTLGLSLLAEPDVNPNESGEAAPIEFQVVVLAEDSKLLATDYDQVTADVEKALGKNYLDHQDYTLLPGQFKYLPPVKLDEKTRYIGVIARYADPDSAEWRKVIKVKSKGTAYQILVHLRLDEVELQKEEE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9974	pos: 16-17. LAG-CT.	MIRALMLGAALLTLAG

Protein sequence: 172 a.a.

						>T6CP016036 NZ_CP012504:c2471820-2471305 [Aeromonas veronii] [TssJ]
MIRALMLGAALLTLAGCTTMGKMADVAMNPDIQVGSNDDQPSTLGLSLLAEPDVNPNESGEAAPIEFQVVVLAEDSKLLA
TDYDQVTADVEKALGKNYLDHQDYTLLPGQFKYLPPVKLDEKTRYIGVIARYADPDSAEWRKVIKVKSKGTAYQILVHLR
LDEVELQKEEE*					

Nucleotide sequence: 516 bp

						>T6CP016036 NZ_CP012504:c2471820-2471305 [Aeromonas veronii] [TssJ]
ATGATACGTGCGCTGATGCTGGGGGCGGCTCTGCTGACCCTCGCCGGTTGTACCACCATGGGCAAGATGGCTGACGTGGC
CATGAACCCGGATATCCAGGTGGGGAGCAATGATGACCAGCCCTCCACCCTGGGGCTTAGCCTGTTGGCAGAGCCTGATG
TCAACCCCAACGAGAGCGGGGAGGCGGCACCCATCGAGTTTCAGGTGGTGGTGCTGGCTGAAGATTCCAAGCTGCTCGCT
ACTGACTATGACCAGGTGACGGCGGATGTAGAGAAGGCCCTTGGCAAGAACTACCTGGATCATCAGGACTACACCCTGCT
GCCCGGCCAGTTCAAATACCTGCCTCCCGTCAAGCTTGACGAGAAGACCCGTTACATCGGCGTGATTGCACGCTACGCCG
ATCCGGACAGCGCCGAGTGGCGCAAGGTCATCAAGGTCAAGAGCAAGGGAACGGCCTATCAAATCCTGGTGCACCTGCGC
CTGGATGAAGTGGAATTACAAAAAGAAGAAGAATAA					