SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016041
Component protein type	TssB
T6SS ID (Type)	T6SS01208 (Type i1)
Strain	Aeromonas veronii strain TH0426
Replicon	chromosome
Sequence	Protein sequence (168 a.a.); Nucleotide sequence (504 bp)
Reference	[1] Song H, Kang Y, Qian A, et al. Inactivation of the T6SS inner membrane protein DotU results in severe attenuation and decreased pathogenicity of Aeromonas veronii TH0426. BMC Microbiol. 2020 Apr 3;20(1):76. doi: 10.1186/s12866-020-01743-5. PMID: 32245412

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AMS64_RS11345
Coordinate (Strand)	2477784..2478287 (-)
NCBI ID	WP_021230530.1
RefSeq	NZ_CP012504
Uniprot ID	A0A7Z3TQF7_9GAMM, A0A2S3XL21_9GAMM, A0A0T6V235_9GAMM
KEGG ID	avo:AMS64_11325
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	8.4e-56	10..160

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-167	MGKGIDGATVAPKERINIKYVPATGGQQAEIELPLTMMVVGNMKGRTEETPIEERQTVSIDKNNFSSVMKESALELNFSVPNRLEENSQDEMPVSIKIQSLEDFTPDSVAQQVPELRKLLELREALVALKGPLGNIPSFRNRLQDLLSSDEAREQLLKELDLIKPAE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9856	-	-

Protein sequence: 168 a.a.

						>T6CP016041 NZ_CP012504:c2478287-2477784 [Aeromonas veronii] [TssB]
MGKGIDGATVAPKERINIKYVPATGGQQAEIELPLTMMVVGNMKGRTEETPIEERQTVSIDKNNFSSVMKESALELNFSV
PNRLEENSQDEMPVSIKIQSLEDFTPDSVAQQVPELRKLLELREALVALKGPLGNIPSFRNRLQDLLSSDEAREQLLKEL
DLIKPAE*					

Nucleotide sequence: 504 bp

						>T6CP016041 NZ_CP012504:c2478287-2477784 [Aeromonas veronii] [TssB]
ATGGGAAAAGGAATTGACGGCGCCACAGTTGCGCCCAAAGAACGTATCAATATCAAATATGTACCGGCAACCGGTGGTCA
ACAGGCGGAAATTGAACTTCCGCTGACTATGATGGTGGTTGGTAATATGAAGGGCCGTACCGAAGAAACGCCGATCGAGG
AGCGCCAAACCGTTTCCATCGACAAAAACAACTTTTCTTCCGTCATGAAAGAATCTGCACTGGAACTGAACTTCTCTGTT
CCCAATCGTCTGGAAGAGAACAGCCAGGACGAGATGCCGGTTTCGATCAAAATCCAGTCTCTGGAAGACTTCACTCCTGA
CAGTGTTGCCCAACAGGTCCCCGAGCTGCGCAAGCTGCTTGAACTGCGTGAGGCGCTGGTTGCCCTGAAGGGGCCATTGG
GCAATATCCCTTCTTTCCGCAATCGTTTGCAGGATCTGCTGTCCAGTGATGAGGCTCGTGAGCAACTGCTCAAAGAGCTG
GATTTGATCAAGCCTGCCGAGTGA					