SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016034
Component protein type	TssL
T6SS ID (Type)	T6SS01208 (Type i1)
Strain	Aeromonas veronii strain TH0426
Replicon	chromosome
Sequence	Protein sequence (260 a.a.); Nucleotide sequence (780 bp)
Reference	[1] Song H, Kang Y, Qian A, et al. Inactivation of the T6SS inner membrane protein DotU results in severe attenuation and decreased pathogenicity of Aeromonas veronii TH0426. BMC Microbiol. 2020 Apr 3;20(1):76. doi: 10.1186/s12866-020-01743-5. PMID: 32245412

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AMS64_RS11305
Coordinate (Strand)	2469165..2469944 (-)
NCBI ID	WP_019444943.1
RefSeq	NZ_CP012504
Uniprot ID	A0A1E4WT12_9GAMM, A0A1E4VY53_9GAMM, A0A0T6PF91_9GAMM, A0A0T6V2B0_9GAMM
KEGG ID	avo:AMS64_11285
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	2.6e-62	39..241

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-218	MTTDIINNEQLSDLLFDHAEQLDMDSDYWFRLRGQSINPMIDAVTPLLGLVQRVRLLSRYDRVPELYQRVVTEIQAIEQELMAQGYENGVVLSFRYILCTFIDEAVMGRDWGSQSEWSQHSLLARFHNETWGGEKVFVLLSRLQEDPVRYRDILEFIYLCLCLGFEGRYKVMSQGRDEFERIVKQLHKQLSLERGGEAPSVFHLDLGQQASRYQLRKQ
Transmembrane helix	219-241	VSLRTLFMGGALILAIIFGLYHH
Outside	242-259	QLNNQTQDVLRQLGELLK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9963	-	-

Protein sequence: 260 a.a.

						>T6CP016034 NZ_CP012504:c2469944-2469165 [Aeromonas veronii] [TssL]
MTTDIINNEQLSDLLFDHAEQLDMDSDYWFRLRGQSINPMIDAVTPLLGLVQRVRLLSRYDRVPELYQRVVTEIQAIEQE
LMAQGYENGVVLSFRYILCTFIDEAVMGRDWGSQSEWSQHSLLARFHNETWGGEKVFVLLSRLQEDPVRYRDILEFIYLC
LCLGFEGRYKVMSQGRDEFERIVKQLHKQLSLERGGEAPSVFHLDLGQQASRYQLRKQVSLRTLFMGGALILAIIFGLYH
HQLNNQTQDVLRQLGELLK*					

Nucleotide sequence: 780 bp

						>T6CP016034 NZ_CP012504:c2469944-2469165 [Aeromonas veronii] [TssL]
ATGACCACGGACATTATCAACAACGAGCAACTGAGCGACCTGCTGTTCGATCACGCCGAGCAGCTGGACATGGATTCGGA
CTACTGGTTCCGCCTGCGTGGCCAGAGCATCAACCCGATGATCGATGCGGTGACCCCGCTGCTGGGGCTGGTACAGCGGG
TGCGTCTGTTGTCGCGCTACGACCGGGTGCCCGAGCTTTATCAACGGGTGGTGACCGAGATCCAGGCCATCGAGCAGGAG
TTGATGGCTCAGGGTTACGAGAACGGCGTCGTCCTCTCGTTTCGCTACATCCTCTGTACTTTTATCGATGAGGCGGTGAT
GGGGCGCGATTGGGGCAGCCAGAGCGAATGGTCCCAACACTCCCTGCTGGCCAGGTTTCACAACGAGACCTGGGGTGGCG
AGAAGGTGTTCGTGCTGCTGTCGCGTCTGCAGGAAGATCCGGTGCGCTATCGCGACATCCTGGAGTTCATCTATCTCTGC
CTCTGCCTTGGTTTTGAGGGGCGCTACAAGGTGATGAGCCAGGGCCGTGACGAGTTTGAACGGATCGTCAAGCAACTGCA
CAAGCAGCTGTCACTCGAACGTGGCGGTGAGGCACCCAGCGTGTTTCATCTCGACCTCGGTCAGCAGGCATCTCGCTACC
AGCTGCGCAAGCAAGTCTCCCTGCGCACCCTGTTTATGGGCGGCGCGCTGATCCTGGCCATCATCTTTGGCCTCTATCAC
CACCAACTCAATAACCAGACCCAGGACGTACTGCGTCAACTGGGCGAATTATTAAAATAA					