Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00126  |
| T6SS type | Type i4b |
| Strain | Burkholderia cenocepacia AU 1054 |
| Replicon (RefSeq) | chromosome I (NC_008060) |
| Location | 2880426..2919827 |
| Function | anti-bacterial; anti-eukaryotic |
| Comment | T6SS effector TecA of B. cenocepacia exacerbates inflammatory cell recruitment and weight loss and is required for lethality, acts as a key virulence factor during lung infection. |
| References | [1] Boyer F et al (2009) Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 0.488888889. [PudMed:19284603] [2] Perault AI, Chandler CE, Rasko DA, Ernst RK, Wolfgang MC, Cotter PA. et al (2020) Host Adaptation Predisposes Pseudomonas aeruginosa to Type VI Secretion System-Mediated Predation by the Burkholderia cepacia Complex.. Cell Host Microbe. 28(4):534-547. [PudMed:32755549] [3] Loeven NA, Perault AI, Cotter PA, Hodges CA, Schwartzman JD, Hampton TH, Bliska JB. et al (2021) The Burkholderia cenocepacia Type VI Secretion System Effector TecA Is a Virulence Factor in Mouse Models of Lung Infection.. mBio. 12(5):e0209821. [PudMed:34579569] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| BCEN_RS13040 | 2875714..2876691 | + | 978 | WP_011694129.1 | zinc-binding alcohol dehydrogenase family protein | ||
| BCEN_RS33185 | 2876716..2876868 | + | 153 | WP_011694128.1 | DUF1427 family protein | ||
| BCEN_RS13045 | 2876912..2877940 | - | 1029 | WP_011546564.1 | transporter | ||
| BCEN_RS13050 | 2878153..2879157 | - | 1005 | WP_011546565.1 | hypothetical protein | ||
| BCEN_RS13055 | 2879279..2880124 | + | 846 | WP_011546566.1 | M15 family metallopeptidase | ||
| T6CP001216 | TssM | BCEN_RS13060 (tssM) | 2880426..2884370 | + | 3945 | WP_011546567.1 | type VI secretion system membrane subunit TssM |
| BCEN_RS13065 (tagF) | 2884367..2885356 | + | 990 | WP_006477085.1 | type VI secretion system-associated protein TagF | ||
| BCEN_RS13070 | 2885365..2886309 | + | 945 | WP_050768801.1 | OmpA family protein | ||
| BCEN_RS13075 | 2886577..2887197 | - | 621 | WP_011546569.1 | hypothetical protein | ||
| BCEN_RS13080 | 2887230..2888099 | - | 870 | WP_011546570.1 | hypothetical protein | ||
| BCEN_RS13085 | 2888210..2888533 | + | 324 | pseudogene | OmpA family protein | ||
| BCEN_RS13090 | 2888616..2890877 | - | 2262 | WP_011546572.1 | DUF2235 domain-containing protein | ||
| BCEN_RS13095 | 2890864..2891784 | - | 921 | WP_175810064.1 | hypothetical protein | ||
| BCEN_RS34670 | 2891777..2891986 | - | 210 | WP_041489595.1 | hypothetical protein | ||
| BCEN_RS13100 | 2892019..2892774 | - | 756 | WP_166488154.1 | hypothetical protein | ||
| BCEN_RS13105 | 2892842..2893882 | - | 1041 | WP_011546575.1 | DUF4123 domain-containing protein | ||
| BCEN_RS13110 | 2893882..2896447 | - | 2566 | pseudogene | type VI secretion system tip protein VgrG | ||
| T6CP001218 | TssA | BCEN_RS13115 (tssA) | 2896514..2897635 | - | 1122 | WP_011546577.1 | type VI secretion system protein TssA |
| T6CP011511 | TssH | BCEN_RS13120 (tssH) | 2897679..2900346 | - | 2668 | pseudogene | type VI secretion system ATPase TssH |
| T6CP001220 | TssG | BCEN_RS13125 (tssG) | 2900388..2901488 | - | 1101 | WP_011694123.1 | type VI secretion system baseplate subunit TssG |
| T6CP001221 | TssF | BCEN_RS13130 (tssF) | 2901452..2903287 | - | 1836 | WP_006477091.1 | type VI secretion system baseplate subunit TssF |
| T6CP001222 | TssE | BCEN_RS13135 (tssE) | 2903364..2903849 | - | 486 | WP_006477092.1 | type VI secretion system baseplate subunit TssE |
| T6CP001223 | TssD | BCEN_RS13140 | 2903912..2904415 | - | 504 | WP_006477093.1 | type VI secretion system tube protein Hcp |
| T6CP001224 | TssC | BCEN_RS13145 (tssC) | 2904486..2905976 | - | 1491 | WP_006477094.1 | type VI secretion system contractile sheath large subunit |
| T6CP001225 | TssB | BCEN_RS13150 (tssB) | 2905992..2906507 | - | 516 | WP_006484395.1 | type VI secretion system contractile sheath small subunit |
| BCEN_RS13155 | 2906554..2907189 | - | 636 | WP_011546581.1 | tetratricopeptide repeat protein | ||
| T6CP001226 | TssJ | BCEN_RS13160 (tssJ) | 2907562..2908173 | + | 612 | WP_006477097.1 | type VI secretion system lipoprotein TssJ |
| T6CP001227 | TssK | BCEN_RS13165 (tssK) | 2908276..2909622 | + | 1347 | WP_006477098.1 | type VI secretion system baseplate subunit TssK |
| T6CP001228 | TssL | BCEN_RS13170 (icmH) | 2909619..2910401 | + | 783 | WP_011546582.1 | type IVB secretion system protein IcmH/DotU |
| BCEN_RS13175 | 2910488..2910805 | - | 318 | WP_011546583.1 | hypothetical protein | ||
| BCEN_RS33190 | 2911341..2911916 | - | 576 | WP_011546584.1 | hypothetical protein | ||
| BCEN_RS13180 | 2911927..2916584 | - | 4658 | pseudogene | RHS domain-containing protein | ||
| T6CP001229 | TssI | BCEN_RS13185 | 2916636..2919827 | - | 3192 | WP_011546587.1 | type VI secretion system tip protein VgrG |
| BCEN_RS13190 | 2920184..2920984 | + | 801 | WP_011546588.1 | transporter substrate-binding domain-containing protein | ||
| BCEN_RS13195 | 2921094..2921297 | - | 204 | WP_011546589.1 | hypothetical protein | ||
| BCEN_RS13200 | 2922015..2922966 | - | 952 | pseudogene | sce7725 family protein | ||
| BCEN_RS33195 | 2923030..2923914 | - | 885 | WP_011546591.1 | sce7726 family protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF76636 |
BCEN_RS18170 (tecA) | - | - | Burkholderia cenocepacia AU 1054 | chromosome (NC_008061) | 779302..779781 (+) | WP_011547418.1 |
| EFF76637 |
BCEN_RS13090 (tle1) | Tle | IMU38210 |
Burkholderia cenocepacia AU 1054 | chromosome (NC_008060) | 2888616..2890877 (-) | WP_011546572.1 |
| EFF76638 |
BCEN_RS13180 (tne1) | Tde | IMU38211 |
Burkholderia cenocepacia AU 1054 | chromosome (NC_008060) | 2911927..2916584 (-) | WP_011694122.1 |
| EFF76639 |
BCEN_RS16675 (tne2) | Tde | IMU38212 |
Burkholderia cenocepacia AU 1054 | chromosome (NC_008061) | 433352..437836 (+) | WP_011547150.1 |
| EFF76640 |
BCEN_RS22905 (tpe1) | Tme | IMU38213 |
Burkholderia cenocepacia AU 1054 | chromosome (NC_008061) | 1873432..1876008 (-) | WP_011548259.1 |
| EFF76641 |
BCEN_RS06980 (tle2) | Tle | IMU38214 |
Burkholderia cenocepacia AU 1054 | chromosome (NC_008060) | 1552877..1554958 (-) | WP_011545611.1 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|
| IMU38210 |
BCEN_RS13095 (tli1) | EFF76637 |
Burkholderia cenocepacia AU 1054 | chromosome (NC_008060) | 2890864..2891784 (-) | WP_175810064.1 |
| IMU38211 |
BCEN_RS33190 (tni1) | EFF76638 |
Burkholderia cenocepacia AU 1054 | chromosome (NC_008060) | 2911341..2911916 (-) | WP_011546584.1 |
| IMU38212 |
BCEN_RS33450 (tni2) | EFF76639 |
Burkholderia cenocepacia AU 1054 | chromosome (NC_008061) | 437845..438333 (+) | WP_011547151.1 |
| IMU38213 |
BCEN_RS33835 (tpi1) | EFF76640 |
Burkholderia cenocepacia AU 1054 | chromosome (NC_008061) | 1872451..1872882 (-) | WP_077190848.1 |
| IMU38214 |
BCEN_RS06975 (tli2) | EFF76641 |
Burkholderia cenocepacia AU 1054 | chromosome (NC_008060) | 1551205..1552767 (-) | WP_011545610.1 |
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|