Detailed information of immunity protein
Summary
| Immunity protein ID | IMU38213  |
| Cognate effector | EFF76640 |
| Related T6SS (Type) | T6SS00126 (Type i4b) |
| Strain | Burkholderia cenocepacia AU 1054 |
| Replicon | chromosome |
| Sequence | Protein sequence (144 a.a.); Nucleotide sequence (432 bp) |
| Description | The E-I deletion mutant was outcompeted by its parental strain in a T6SS-dependent manner. Ectopic expression of the cognate immunity gene in each mutant rescued it from T6SS-mediated killing by the parental strain, providing evidence that Tle1-Tli1, Tne1-Tni1, Tne2-Tni2, and Tpe1-Tpi1 are true antibacterial E-I pairs associated with the BcAU1054 T6SS. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | BCEN_RS33835 (tpi1) |
| Coordinates (Strand) | 1872451..1872882 (-) |
| NCBI ID | WP_077190848.1 |
| RefSeq | NC_008061 |
| Uniprot ID | A0A427NPJ9_9BURK |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-143 | VVRCLVLDDKGMVKDTFSMGTRVVLSSDEGSVGGQEVMKVLYQDFEFYRRFMEEGPASLPPVTEFLPKGASLRNSLRLNFDGTSGLLKSGNPIVWLVVAVGALPAFSQSLLHWLAQLTCREPVWPDDIERACNAATPSNGLTA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9826 | - | - |
Protein sequence: 144 a.a. .
>IMU38213 NC_008061:c1872882-1872451 [Burkholderia cenocepacia AU 1054]
VVRCLVLDDKGMVKDTFSMGTRVVLSSDEGSVGGQEVMKVLYQDFEFYRRFMEEGPASLPPVTEFLPKGASLRNSLRLNF
DGTSGLLKSGNPIVWLVVAVGALPAFSQSLLHWLAQLTCREPVWPDDIERACNAATPSNGLTA*
VVRCLVLDDKGMVKDTFSMGTRVVLSSDEGSVGGQEVMKVLYQDFEFYRRFMEEGPASLPPVTEFLPKGASLRNSLRLNF
DGTSGLLKSGNPIVWLVVAVGALPAFSQSLLHWLAQLTCREPVWPDDIERACNAATPSNGLTA*
Nucleotide sequence: 432 bp .
>IMU38213 NC_008061:c1872882-1872451 [Burkholderia cenocepacia AU 1054]
GTGGTTCGTTGCCTGGTTCTTGACGACAAGGGGATGGTCAAGGATACATTCTCGATGGGAACGCGAGTTGTCCTGTCGTC
TGATGAGGGAAGCGTCGGGGGACAGGAGGTCATGAAAGTCCTCTACCAGGACTTTGAGTTCTACCGGCGATTTATGGAAG
AAGGCCCCGCTTCGCTGCCCCCGGTGACCGAGTTCCTGCCGAAGGGAGCCTCCCTGCGCAATTCGCTGAGGTTGAATTTC
GACGGAACCTCGGGCCTACTTAAGTCGGGCAATCCGATAGTGTGGCTGGTGGTGGCGGTCGGGGCACTTCCCGCATTCTC
TCAATCCCTATTGCATTGGCTCGCCCAACTGACTTGCCGCGAACCGGTATGGCCGGACGATATCGAGCGGGCGTGCAATG
CTGCGACGCCGTCCAACGGCCTTACAGCATGA
GTGGTTCGTTGCCTGGTTCTTGACGACAAGGGGATGGTCAAGGATACATTCTCGATGGGAACGCGAGTTGTCCTGTCGTC
TGATGAGGGAAGCGTCGGGGGACAGGAGGTCATGAAAGTCCTCTACCAGGACTTTGAGTTCTACCGGCGATTTATGGAAG
AAGGCCCCGCTTCGCTGCCCCCGGTGACCGAGTTCCTGCCGAAGGGAGCCTCCCTGCGCAATTCGCTGAGGTTGAATTTC
GACGGAACCTCGGGCCTACTTAAGTCGGGCAATCCGATAGTGTGGCTGGTGGTGGCGGTCGGGGCACTTCCCGCATTCTC
TCAATCCCTATTGCATTGGCTCGCCCAACTGACTTGCCGCGAACCGGTATGGCCGGACGATATCGAGCGGGCGTGCAATG
CTGCGACGCCGTCCAACGGCCTTACAGCATGA