Detailed information of immunity protein


Summary

Immunity protein ID IMU38213 experimental
Cognate effector EFF76640 experimental
Related T6SS (Type) T6SS00126 experimental (Type i4b)
Strain Burkholderia cenocepacia AU 1054
Replicon chromosome
Sequence Protein sequence (144 a.a.); Nucleotide sequence (432 bp)
Description The E-I deletion mutant was outcompeted by its parental strain in a T6SS-dependent manner. Ectopic expression of the cognate immunity gene in each mutant rescued it from T6SS-mediated killing by the parental strain, providing evidence that Tle1-Tli1, Tne1-Tni1, Tne2-Tni2, and Tpe1-Tpi1 are true antibacterial E-I pairs associated with the BcAU1054 T6SS.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) BCEN_RS33835 (tpi1)
Coordinates (Strand) 1872451..1872882 (-)
NCBI ID WP_077190848.1
RefSeq NC_008061
Uniprot ID A0A427NPJ9_9BURK
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-143 VVRCLVLDDKGMVKDTFSMGTRVVLSSDEGSVGGQEVMKVLYQDFEFYRRFMEEGPASLPPVTEFLPKGASLRNSLRLNFDGTSGLLKSGNPIVWLVVAVGALPAFSQSLLHWLAQLTCREPVWPDDIERACNAATPSNGLTA



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9826 - -



  Protein sequence: 144 a.a.    .

>IMU38213 NC_008061:c1872882-1872451 [Burkholderia cenocepacia AU 1054]
VVRCLVLDDKGMVKDTFSMGTRVVLSSDEGSVGGQEVMKVLYQDFEFYRRFMEEGPASLPPVTEFLPKGASLRNSLRLNF
DGTSGLLKSGNPIVWLVVAVGALPAFSQSLLHWLAQLTCREPVWPDDIERACNAATPSNGLTA*

  Nucleotide sequence: 432 bp    .

>IMU38213 NC_008061:c1872882-1872451 [Burkholderia cenocepacia AU 1054]
GTGGTTCGTTGCCTGGTTCTTGACGACAAGGGGATGGTCAAGGATACATTCTCGATGGGAACGCGAGTTGTCCTGTCGTC
TGATGAGGGAAGCGTCGGGGGACAGGAGGTCATGAAAGTCCTCTACCAGGACTTTGAGTTCTACCGGCGATTTATGGAAG
AAGGCCCCGCTTCGCTGCCCCCGGTGACCGAGTTCCTGCCGAAGGGAGCCTCCCTGCGCAATTCGCTGAGGTTGAATTTC
GACGGAACCTCGGGCCTACTTAAGTCGGGCAATCCGATAGTGTGGCTGGTGGTGGCGGTCGGGGCACTTCCCGCATTCTC
TCAATCCCTATTGCATTGGCTCGCCCAACTGACTTGCCGCGAACCGGTATGGCCGGACGATATCGAGCGGGCGTGCAATG
CTGCGACGCCGTCCAACGGCCTTACAGCATGA