Detailed information of effector


Summary

Effector ID EFF76640 experimental
Effector type Tme
Cognate immunity protein IMU38213 experimental
Related T6SS (Type) T6SS00126 experimental (Type i4b)
Strain Burkholderia cenocepacia AU 1054
Replicon chromosome
Sequence Protein sequence (859 a.a.); Nucleotide sequence (2577 bp)
Description The E-I deletion mutant was outcompeted by its parental strain in a T6SS-dependent manner. Ectopic expression of the cognate immunity gene in each mutant rescued it from T6SS-mediated killing by the parental strain, providing evidence that Tle1-Tli1, Tne1-Tni1, Tne2-Tni2, and Tpe1-Tpi1 are true antibacterial E-I pairs associated with the BcAU1054 T6SS.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) BCEN_RS22905 (tpe1)
Coordinates (Strand) 1873432..1876008 (-)
NCBI ID WP_011548259.1
RefSeq NC_008061
Uniprot ID A0A0H2XWX1_BURCA, A0A3R9C645_9BURK
KEGG ID bcn:Bcen_4615
PDB ID -
Pfam domain hit(s)

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Domain
Pfam ID
E-value
Aligned region
No matching records found
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Outside1-740MALDTNFASISQLTQSAMQTSSRAPNSCNMCKKKGLPILPVRYGVVANSKAHSSAGAMSLMGGKFGDGVVGIALKKAKYTLRTLRYGYVYIFYPKTQRWQIYAVTAEGYLYDYPVGIKLDRSFEKPFSCHQPGHAQLAQCITVEDAEHAGTVYLAFSDELWTQKVRDTYAKNFMGCRDKRMQKFDATAWYNGSMNQPHADSLDHLEGLLSEYKGGRPDALVTGCFPYHDRAGQQPALKKAMDSILPDKGLFFALWDPVGITQELNFEQRVAFGAALAPYEHGIWAASAIDALQKAVAVSAKEDVERAAGMLEANTYEGMGLGALFDGGKALEKRLKSIEAQKESELATAGPEAWKPYTTHYRQQAIVDFRQQMANAMKSEQASTLIPLSQDHQAWLSSAALLNVFESDYEPGSWRCGLNYTNTFTRCIEGSADRKNAVDVLVKWAKGSVSDTRNPLLRAFVLNSPVLAEKVKEASGYPYMELREFVAKLIESYSKIEGVEESKDKGIKVAVAKALAWLLHETGGPVAEAISASVDTAATKVLYATLCMRTKQLFEFKVVYGSVNQWVNYIARQMQEVMPGRQKISVQDLKKQLQDKANAGGGADPQIKAKQFVPVESPATEAAALAGVKAKPGFEKLASATLTPDVVETIYMPKFRLMTKGEPGFAGIGAIFSAINLRFAREELKKANRFNQSEAGIKFDNAIGGLVASVAQYGSAAIDTVGKAGAQLGTGWLRLERFLN
Transmembrane helix741-763VAGRYGGAVVGLVSAAIDIYHAV
Inside764-771QDFKEGNV
Transmembrane helix772-794TMFILDAASAVVGLALAYSVIFL
Outside795-797SAS
Transmembrane helix798-820AAAVVALPLLLIAALIGILINYF
Inside821-858KGKEVDEWLERCFFGLKEAKERFPTLAEDQKAFTAMLS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
Other0.8591--


  Protein sequence: 859 a.a.    .

>EFF76640 NC_008061:c1876008-1873432 Type VI Membrane-disrupting Effector (Tme) [Burkholderia cenocepacia AU 1054]
MALDTNFASISQLTQSAMQTSSRAPNSCNMCKKKGLPILPVRYGVVANSKAHSSAGAMSLMGGKFGDGVVGIALKKAKYT
LRTLRYGYVYIFYPKTQRWQIYAVTAEGYLYDYPVGIKLDRSFEKPFSCHQPGHAQLAQCITVEDAEHAGTVYLAFSDEL
WTQKVRDTYAKNFMGCRDKRMQKFDATAWYNGSMNQPHADSLDHLEGLLSEYKGGRPDALVTGCFPYHDRAGQQPALKKA
MDSILPDKGLFFALWDPVGITQELNFEQRVAFGAALAPYEHGIWAASAIDALQKAVAVSAKEDVERAAGMLEANTYEGMG
LGALFDGGKALEKRLKSIEAQKESELATAGPEAWKPYTTHYRQQAIVDFRQQMANAMKSEQASTLIPLSQDHQAWLSSAA
LLNVFESDYEPGSWRCGLNYTNTFTRCIEGSADRKNAVDVLVKWAKGSVSDTRNPLLRAFVLNSPVLAEKVKEASGYPYM
ELREFVAKLIESYSKIEGVEESKDKGIKVAVAKALAWLLHETGGPVAEAISASVDTAATKVLYATLCMRTKQLFEFKVVY
GSVNQWVNYIARQMQEVMPGRQKISVQDLKKQLQDKANAGGGADPQIKAKQFVPVESPATEAAALAGVKAKPGFEKLASA
TLTPDVVETIYMPKFRLMTKGEPGFAGIGAIFSAINLRFAREELKKANRFNQSEAGIKFDNAIGGLVASVAQYGSAAIDT
VGKAGAQLGTGWLRLERFLNVAGRYGGAVVGLVSAAIDIYHAVQDFKEGNVTMFILDAASAVVGLALAYSVIFLSASAAA
VVALPLLLIAALIGILINYFKGKEVDEWLERCFFGLKEAKERFPTLAEDQKAFTAMLS*

  Nucleotide sequence: 2577 bp    .

>EFF76640 NC_008061:c1876008-1873432 Type VI Membrane-disrupting Effector (Tme) [Burkholderia cenocepacia AU 1054]
ATGGCACTCGACACGAATTTTGCGAGTATTTCGCAGCTCACGCAGTCCGCGATGCAGACCAGCTCGCGTGCGCCGAATTC
ATGCAACATGTGCAAGAAGAAGGGATTGCCGATTCTGCCTGTCCGCTACGGGGTCGTGGCCAACTCCAAGGCTCACAGCT
CTGCTGGCGCGATGTCGCTCATGGGCGGAAAGTTTGGCGACGGCGTCGTCGGCATCGCGCTCAAAAAGGCAAAGTACACG
CTGCGCACCCTGCGCTACGGCTACGTGTATATCTTCTATCCAAAGACACAGCGATGGCAGATATACGCGGTTACGGCGGA
AGGATACTTGTACGACTATCCGGTCGGCATCAAGCTCGACCGTAGTTTCGAGAAACCGTTCAGCTGCCACCAACCTGGGC
ATGCGCAACTGGCTCAATGCATCACCGTTGAAGATGCAGAACATGCCGGAACCGTGTATCTGGCGTTCAGCGATGAGCTT
TGGACACAGAAAGTCCGCGACACCTACGCGAAGAACTTCATGGGTTGTCGTGACAAGCGGATGCAGAAGTTCGATGCAAC
CGCCTGGTATAACGGCAGCATGAACCAGCCGCACGCGGATAGCCTGGACCATCTCGAGGGGCTACTGAGCGAATACAAGG
GCGGTCGTCCCGATGCGCTCGTTACCGGTTGCTTTCCGTACCATGACCGTGCCGGGCAGCAGCCGGCCCTCAAAAAGGCG
ATGGATTCCATACTGCCGGACAAGGGGCTGTTCTTCGCGTTGTGGGACCCGGTTGGCATCACGCAGGAACTCAATTTTGA
GCAGCGCGTTGCTTTCGGCGCAGCGCTTGCGCCGTATGAACACGGAATATGGGCCGCCAGCGCAATCGACGCGCTGCAAA
AGGCGGTCGCGGTTTCGGCGAAAGAGGACGTGGAACGTGCCGCCGGCATGCTGGAAGCCAATACTTATGAGGGCATGGGC
CTCGGGGCTCTGTTCGATGGTGGCAAGGCGCTCGAGAAGCGGCTCAAAAGCATTGAGGCGCAGAAGGAGTCTGAACTTGC
AACAGCCGGACCGGAAGCATGGAAGCCCTACACGACCCACTATCGGCAGCAGGCTATCGTCGACTTTCGGCAACAGATGG
CGAACGCGATGAAGTCGGAGCAGGCGAGCACGCTTATACCGCTGTCGCAAGACCATCAGGCGTGGCTGTCCAGTGCCGCA
TTGCTGAACGTTTTCGAGTCGGATTACGAGCCGGGAAGCTGGAGATGTGGGCTGAACTATACCAACACATTTACACGATG
CATCGAAGGCTCGGCTGACCGCAAGAATGCCGTCGATGTGCTCGTCAAATGGGCTAAAGGCAGCGTATCTGATACTCGCA
ATCCATTGTTGCGGGCGTTCGTCCTAAACAGCCCGGTGCTGGCTGAGAAGGTCAAGGAGGCGTCCGGCTATCCGTACATG
GAACTGAGGGAGTTCGTCGCGAAGCTCATAGAGTCGTACAGCAAGATAGAGGGCGTGGAAGAGTCGAAGGATAAGGGCAT
CAAAGTTGCTGTTGCGAAGGCGTTAGCATGGCTTCTTCATGAGACAGGTGGGCCCGTGGCCGAAGCTATCAGCGCGAGTG
TAGATACGGCTGCAACCAAAGTGCTGTATGCGACTCTGTGCATGCGAACGAAACAGCTGTTCGAGTTTAAGGTCGTATAC
GGTTCGGTAAACCAATGGGTGAACTACATCGCCCGTCAGATGCAGGAAGTGATGCCGGGCCGGCAGAAAATCAGTGTCCA
GGACCTGAAAAAGCAGCTTCAGGACAAAGCCAATGCAGGCGGAGGCGCCGACCCGCAAATTAAGGCGAAGCAGTTCGTGC
CAGTCGAAAGCCCGGCGACAGAGGCAGCTGCCCTGGCTGGCGTCAAAGCCAAACCCGGCTTCGAGAAATTGGCCAGTGCG
ACGTTGACACCCGACGTGGTCGAGACGATTTACATGCCGAAATTTAGGCTCATGACGAAGGGGGAGCCTGGGTTTGCGGG
CATCGGGGCGATATTCAGTGCTATCAATCTTCGCTTTGCTCGGGAAGAATTGAAGAAGGCGAATCGGTTCAATCAATCGG
AGGCCGGCATCAAGTTCGACAACGCCATTGGTGGACTCGTGGCGAGTGTTGCGCAATATGGTTCGGCTGCAATTGACACC
GTCGGAAAGGCTGGGGCTCAGTTGGGTACAGGCTGGCTTCGACTAGAACGTTTCCTCAACGTCGCGGGGAGGTACGGCGG
CGCGGTGGTTGGGCTCGTGTCTGCTGCGATTGACATCTATCATGCCGTGCAGGATTTCAAGGAAGGTAATGTGACGATGT
TTATCCTGGATGCCGCATCAGCGGTTGTCGGTCTTGCTCTCGCTTACTCTGTCATATTCCTTTCGGCATCCGCTGCAGCG
GTCGTGGCCCTGCCGCTTCTGCTTATCGCTGCCCTTATTGGTATCCTCATCAACTACTTCAAGGGTAAGGAAGTGGATGA
GTGGCTTGAGCGATGTTTTTTTGGGCTGAAAGAGGCCAAAGAGCGGTTCCCCACTCTGGCCGAAGACCAAAAGGCATTTA
CGGCAATGCTTTCGTGA