Detailed information of effector


Summary

Effector ID EFF76637 experimental
Effector type Tle
Cognate immunity protein IMU38210 experimental
Related T6SS (Type) T6SS00126 experimental (Type i4b)
Strain Burkholderia cenocepacia AU 1054
Replicon chromosome
Sequence Protein sequence (754 a.a.); Nucleotide sequence (2262 bp)
Description The E-I deletion mutant was outcompeted by its parental strain in a T6SS-dependent manner. Ectopic expression of the cognate immunity gene in each mutant rescued it from T6SS-mediated killing by the parental strain, providing evidence that Tle1-Tli1, Tne1-Tni1, Tne2-Tni2, and Tpe1-Tpi1 are true antibacterial E-I pairs associated with the BcAU1054 T6SS.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) BCEN_RS13090 (tle1)
Coordinates (Strand) 2888616..2890877 (-)
NCBI ID WP_011546572.1
RefSeq NC_008060
Uniprot ID A0A0H2XTR0_BURCA
KEGG ID bcn:Bcen_2626
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DUF2235 PF09994.11 6e-08 329..441
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-753 MAGANDRNAIRLDPDDATPQSVRMAVDKMATANYPKGDCLPCGATIRIGVFFDAFGRSRDEDDPDSGLYSNISRLFDAHRTNLDVDRPVNQFWFRLYYSGLGTDLNADAKSNDWLNAGLAAGKMVGKKVLAATKGVAESVARVNQPIKQLDVKGRVTTATKQSASEFSFRPMLNAFNDFKKDIQNIPRTARRIANVLDASPERIWERTKAGGIAVFRNAKNDAKRNPLKIVQSTVRQVITGIAIENVPIVRDAQVMSAYFGTGVDSRLVAAKDQLEKAVEAVKGQASKIRKIEVSVFGADRGCVLARAFVNDLATKYKRRDDQDLALNGISLEIKFVGLLDAVSSIMSEEAGEIIGILPFLGTIKPNYKDRSLAVPASVQRCVHFAAAHEMRMYQRLDSLEKTRAEQRLYPGTSSDIAGGSLHGSLGFNAELMRIPLRDMLFEALKAGSALLDMETLAKRRPDVFSRFSIATPITYDGKQYRIKALVDAYRKVVPDTQGVNFLPHAEVFLRWIAVRYQDPEFRHSVTDPATEWRKDVERADYDRRDAKAKLDWAMMGRQRMDSPEIQKLKLAKQAADKRYNDLWKDVPPSNYTTVWQRLDKEAKEMTGKWEKMRPEYEAEQQRRFTQSFNNKPTYTPLFLAPAFARHVELNEDEVALVRAWEQGYAGKNPLPSEVMAMFDMLVHDTLLTSWQDHVLAPSLYFRTRDKDVFGKTDYAKEDKQRKSDDLTAERVDQASKQSQQWARGYQGTPPTH


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9817 - -


  Protein sequence: 754 a.a.    .

>EFF76637 NC_008060:c2890877-2888616 Type VI Lipase Effector (Tle) [Burkholderia cenocepacia AU 1054]
MAGANDRNAIRLDPDDATPQSVRMAVDKMATANYPKGDCLPCGATIRIGVFFDAFGRSRDEDDPDSGLYSNISRLFDAHR
TNLDVDRPVNQFWFRLYYSGLGTDLNADAKSNDWLNAGLAAGKMVGKKVLAATKGVAESVARVNQPIKQLDVKGRVTTAT
KQSASEFSFRPMLNAFNDFKKDIQNIPRTARRIANVLDASPERIWERTKAGGIAVFRNAKNDAKRNPLKIVQSTVRQVIT
GIAIENVPIVRDAQVMSAYFGTGVDSRLVAAKDQLEKAVEAVKGQASKIRKIEVSVFGADRGCVLARAFVNDLATKYKRR
DDQDLALNGISLEIKFVGLLDAVSSIMSEEAGEIIGILPFLGTIKPNYKDRSLAVPASVQRCVHFAAAHEMRMYQRLDSL
EKTRAEQRLYPGTSSDIAGGSLHGSLGFNAELMRIPLRDMLFEALKAGSALLDMETLAKRRPDVFSRFSIATPITYDGKQ
YRIKALVDAYRKVVPDTQGVNFLPHAEVFLRWIAVRYQDPEFRHSVTDPATEWRKDVERADYDRRDAKAKLDWAMMGRQR
MDSPEIQKLKLAKQAADKRYNDLWKDVPPSNYTTVWQRLDKEAKEMTGKWEKMRPEYEAEQQRRFTQSFNNKPTYTPLFL
APAFARHVELNEDEVALVRAWEQGYAGKNPLPSEVMAMFDMLVHDTLLTSWQDHVLAPSLYFRTRDKDVFGKTDYAKEDK
QRKSDDLTAERVDQASKQSQQWARGYQGTPPTH*

  Nucleotide sequence: 2262 bp    .

>EFF76637 NC_008060:c2890877-2888616 Type VI Lipase Effector (Tle) [Burkholderia cenocepacia AU 1054]
ATGGCCGGGGCTAACGATCGAAACGCCATTCGACTCGATCCGGACGACGCTACGCCGCAGAGCGTGCGAATGGCAGTCGA
CAAGATGGCGACCGCCAACTATCCAAAGGGCGACTGTCTGCCGTGCGGGGCAACGATCCGTATCGGCGTATTCTTCGATG
CGTTCGGTCGCAGCAGGGACGAAGACGATCCGGACAGCGGACTGTACTCGAACATCTCGCGTCTTTTCGATGCGCACCGC
ACCAACCTGGACGTCGATCGTCCCGTGAATCAGTTCTGGTTCCGTCTCTACTATTCGGGCCTCGGGACGGATCTCAACGC
GGATGCCAAGTCGAACGACTGGCTCAATGCAGGCCTAGCCGCGGGGAAAATGGTCGGCAAGAAGGTGCTCGCCGCAACGA
AAGGCGTTGCGGAGTCAGTCGCCCGTGTGAACCAGCCGATCAAGCAACTGGACGTGAAAGGTCGCGTGACGACGGCAACC
AAGCAGTCCGCCTCGGAGTTCTCGTTTCGGCCGATGCTGAATGCTTTCAACGACTTCAAGAAGGACATCCAGAACATCCC
CCGTACCGCGCGCCGCATCGCGAACGTACTGGACGCGTCGCCGGAGCGAATCTGGGAGCGCACGAAGGCAGGCGGCATCG
CGGTGTTCCGGAATGCAAAGAACGACGCGAAGCGCAATCCGCTCAAGATCGTGCAATCGACAGTCCGTCAGGTCATTACG
GGAATCGCGATCGAAAACGTACCGATCGTGCGCGATGCGCAGGTGATGAGCGCGTACTTCGGCACCGGCGTCGATTCGCG
GCTCGTTGCCGCGAAGGACCAGTTGGAAAAAGCGGTCGAGGCGGTCAAGGGGCAGGCGTCGAAGATTCGAAAGATCGAAG
TATCGGTGTTTGGTGCCGATCGTGGTTGCGTGCTGGCACGCGCGTTCGTCAACGATCTGGCGACCAAGTACAAGCGTCGC
GATGATCAGGATCTTGCGCTCAACGGCATTTCGCTCGAAATAAAGTTCGTCGGTCTACTGGACGCGGTGTCGTCGATCAT
GTCGGAAGAGGCCGGAGAGATTATCGGTATCCTGCCGTTTCTCGGAACCATCAAGCCGAATTACAAGGACCGGTCGCTCG
CCGTGCCTGCAAGTGTGCAGCGATGCGTGCATTTCGCCGCCGCTCACGAGATGCGCATGTACCAGCGCCTCGACAGCCTG
GAAAAGACCCGTGCCGAGCAACGGCTCTATCCGGGAACGAGCAGCGACATCGCGGGCGGTTCGTTGCATGGCTCGCTGGG
CTTCAATGCGGAGCTGATGCGTATTCCGCTCAGGGACATGCTGTTCGAAGCGCTCAAGGCCGGTAGTGCGCTTCTCGACA
TGGAAACGCTGGCCAAGCGGCGGCCTGACGTATTCAGCCGTTTCTCGATTGCGACGCCGATCACCTACGACGGCAAGCAG
TACAGGATCAAGGCGCTTGTTGATGCGTACCGCAAGGTCGTTCCGGACACGCAAGGCGTGAACTTTCTGCCGCACGCGGA
GGTATTCCTGCGCTGGATCGCCGTGCGATACCAGGATCCGGAATTCCGTCATTCCGTGACTGACCCGGCGACCGAATGGC
GCAAGGACGTCGAAAGAGCCGACTACGATCGGCGCGACGCAAAGGCGAAGCTCGACTGGGCGATGATGGGCCGCCAGCGA
ATGGACTCGCCCGAGATCCAGAAGCTTAAGCTCGCGAAGCAGGCGGCGGACAAGCGTTACAACGATTTATGGAAGGATGT
CCCACCGAGCAACTACACGACGGTCTGGCAGCGTCTCGACAAGGAGGCGAAGGAGATGACCGGCAAGTGGGAGAAGATGC
GGCCGGAATACGAGGCCGAGCAACAGCGCCGCTTCACGCAGTCGTTCAACAACAAGCCGACTTATACGCCACTGTTCCTT
GCGCCCGCGTTTGCACGCCATGTCGAGCTGAACGAGGACGAGGTTGCGCTGGTCAGGGCCTGGGAGCAGGGGTATGCGGG
GAAGAACCCGCTTCCGTCCGAGGTGATGGCGATGTTCGACATGCTCGTCCACGATACGTTGCTGACGAGTTGGCAGGATC
ACGTGCTCGCGCCGTCACTGTACTTCAGGACACGGGACAAGGACGTGTTCGGAAAGACGGATTATGCGAAGGAAGACAAG
CAGCGCAAGAGTGACGATCTCACTGCCGAACGGGTCGACCAAGCGAGCAAGCAGAGCCAGCAATGGGCGAGAGGCTATCA
GGGGACGCCACCGACACATTGA