Detailed information of immunity protein


Summary

Immunity protein ID IMU38210 experimental
Cognate effector EFF76637 experimental
Related T6SS (Type) T6SS00126 experimental (Type i4b)
Strain Burkholderia cenocepacia AU 1054
Replicon chromosome
Sequence Protein sequence (307 a.a.); Nucleotide sequence (921 bp)
Description The E-I deletion mutant was outcompeted by its parental strain in a T6SS-dependent manner. Ectopic expression of the cognate immunity gene in each mutant rescued it from T6SS-mediated killing by the parental strain, providing evidence that Tle1-Tli1, Tne1-Tni1, Tne2-Tni2, and Tpe1-Tpi1 are true antibacterial E-I pairs associated with the BcAU1054 T6SS.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) BCEN_RS13095 (tli1)
Coordinates (Strand) 2890864..2891784 (-)
NCBI ID WP_175810064.1
RefSeq NC_008060
Uniprot ID UPI0002D67D90
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-306 MPDFMQYAAPKFHAIVPDRHGARAARGRFLAALRVAAAGLLFVSMAACSKSDPTYTSISTEALNYLPYNLVRFTITDQFGNKARGGGDLEPGAGEGSIACCYLLKGNDFKVQWTYYDADDWRPGEQVKKQQAEANVSLPPTKEPDSIGSRILEVHFYPDHHVELVFPGEMLGSTRLPIVDVSRDLTKQYGKRLDKKYEDNDAQSHRRISRTVAEAWIKYRFTDRSDLEQYAYYALLVNPRFDAHPAVQKLIQSSKGKPGAFAKAVAALSPTVVKELGQNRFQAVAVPSIPAGLVPPPREENSHGRG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.6385 pos: 47-48. MAA-CS. MPDFMQYAAPKFHAIVPDRHGARAARGRFLAALRVAAAGLLFVSMAA


  Protein sequence: 307 a.a.    .

>IMU38210 NC_008060:c2891784-2890864 [Burkholderia cenocepacia AU 1054]
MPDFMQYAAPKFHAIVPDRHGARAARGRFLAALRVAAAGLLFVSMAACSKSDPTYTSISTEALNYLPYNLVRFTITDQFG
NKARGGGDLEPGAGEGSIACCYLLKGNDFKVQWTYYDADDWRPGEQVKKQQAEANVSLPPTKEPDSIGSRILEVHFYPDH
HVELVFPGEMLGSTRLPIVDVSRDLTKQYGKRLDKKYEDNDAQSHRRISRTVAEAWIKYRFTDRSDLEQYAYYALLVNPR
FDAHPAVQKLIQSSKGKPGAFAKAVAALSPTVVKELGQNRFQAVAVPSIPAGLVPPPREENSHGRG*

  Nucleotide sequence: 921 bp    .

>IMU38210 NC_008060:c2891784-2890864 [Burkholderia cenocepacia AU 1054]
ATGCCTGATTTCATGCAATACGCTGCACCGAAATTCCACGCGATCGTTCCAGACCGTCACGGCGCACGCGCAGCTCGCGG
ACGGTTCCTCGCCGCGTTGCGCGTCGCCGCCGCAGGCTTGCTGTTCGTGTCGATGGCGGCGTGCAGCAAGTCCGATCCGA
CCTACACCAGCATTTCAACGGAGGCGCTGAACTACCTGCCATACAACCTCGTTCGGTTCACGATTACCGACCAGTTCGGC
AACAAGGCACGCGGTGGTGGCGACCTGGAACCCGGGGCCGGGGAAGGCAGCATCGCCTGCTGTTACTTGCTGAAGGGCAA
CGATTTCAAGGTTCAGTGGACGTATTACGATGCGGACGACTGGCGGCCGGGTGAGCAGGTGAAGAAACAGCAAGCGGAGG
CGAACGTTTCGTTGCCGCCAACCAAGGAGCCGGATTCGATCGGCTCGCGCATTCTCGAGGTCCACTTCTACCCCGATCAT
CATGTCGAACTGGTATTTCCTGGCGAGATGCTGGGCAGTACCAGATTGCCGATCGTGGACGTGTCGCGTGACTTGACGAA
ACAGTACGGCAAGCGGCTCGACAAGAAGTACGAGGACAATGACGCTCAGTCGCACCGCAGGATTTCGAGAACCGTTGCGG
AAGCCTGGATCAAGTACCGCTTCACAGATCGCAGCGACCTCGAGCAATACGCCTATTACGCGCTTCTGGTGAATCCTCGG
TTCGACGCGCACCCGGCGGTGCAGAAGCTCATCCAGTCGAGCAAGGGGAAACCGGGCGCATTCGCGAAGGCGGTCGCCGC
CCTGTCACCGACCGTTGTGAAGGAGCTTGGGCAGAACCGATTCCAGGCGGTTGCGGTCCCGTCGATTCCGGCGGGCCTGG
TGCCGCCGCCACGTGAGGAGAATAGCCATGGCCGGGGCTAA