Detailed information of immunity protein
Summary
| Immunity protein ID | IMU38212  |
| Cognate effector | EFF76639 |
| Related T6SS (Type) | T6SS00126 (Type i4b) |
| Strain | Burkholderia cenocepacia AU 1054 |
| Replicon | chromosome |
| Sequence | Protein sequence (163 a.a.); Nucleotide sequence (489 bp) |
| Description | The E-I deletion mutant was outcompeted by its parental strain in a T6SS-dependent manner. Ectopic expression of the cognate immunity gene in each mutant rescued it from T6SS-mediated killing by the parental strain, providing evidence that Tle1-Tli1, Tne1-Tni1, Tne2-Tni2, and Tpe1-Tpi1 are true antibacterial E-I pairs associated with the BcAU1054 T6SS. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | BCEN_RS33450 (tni2) |
| Coordinates (Strand) | 437845..438333 (+) |
| NCBI ID | WP_011547151.1 |
| RefSeq | NC_008061 |
| Uniprot ID | UPI000052D5A9 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-162 | MRSIKEIVEIIRSDGRCQVLPASGKLPQLPHADMAYPNDMVEFYNLCGGVILFRPGKDNISFKILPAGDVLQTNMIIVGDPCEDDISFSWYAICKTDNGDYISIDTSKERNGRCYDSNFEVHGVVGSCPIIALSFSELLDELYKSSGADIFWKAKDYGDAYA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9622 | - | - |
Protein sequence: 163 a.a. .
>IMU38212 NC_008061:437845-438333 [Burkholderia cenocepacia AU 1054]
MRSIKEIVEIIRSDGRCQVLPASGKLPQLPHADMAYPNDMVEFYNLCGGVILFRPGKDNISFKILPAGDVLQTNMIIVGD
PCEDDISFSWYAICKTDNGDYISIDTSKERNGRCYDSNFEVHGVVGSCPIIALSFSELLDELYKSSGADIFWKAKDYGDA
YA*
MRSIKEIVEIIRSDGRCQVLPASGKLPQLPHADMAYPNDMVEFYNLCGGVILFRPGKDNISFKILPAGDVLQTNMIIVGD
PCEDDISFSWYAICKTDNGDYISIDTSKERNGRCYDSNFEVHGVVGSCPIIALSFSELLDELYKSSGADIFWKAKDYGDA
YA*
Nucleotide sequence: 489 bp .
>IMU38212 NC_008061:437845-438333 [Burkholderia cenocepacia AU 1054]
ATGAGGTCGATTAAGGAAATTGTCGAGATCATAAGATCCGATGGTAGATGCCAAGTGTTGCCGGCGAGCGGAAAGCTACC
GCAGCTGCCACATGCCGACATGGCATATCCGAATGATATGGTCGAATTCTACAATCTTTGCGGTGGTGTGATTTTGTTCA
GACCCGGGAAAGATAATATTTCTTTCAAGATATTACCTGCCGGTGATGTGTTGCAAACCAATATGATCATTGTGGGTGAT
CCGTGTGAGGACGATATTTCTTTTTCGTGGTATGCAATCTGTAAAACAGACAACGGTGACTACATATCCATTGATACCTC
CAAGGAAAGGAATGGAAGATGTTACGATAGTAATTTCGAAGTTCATGGTGTAGTTGGGAGTTGTCCAATAATAGCGTTAA
GTTTTTCTGAGTTGCTTGATGAGCTATATAAAAGTTCGGGGGCTGATATTTTTTGGAAAGCGAAGGATTACGGCGACGCT
TACGCGTGA
ATGAGGTCGATTAAGGAAATTGTCGAGATCATAAGATCCGATGGTAGATGCCAAGTGTTGCCGGCGAGCGGAAAGCTACC
GCAGCTGCCACATGCCGACATGGCATATCCGAATGATATGGTCGAATTCTACAATCTTTGCGGTGGTGTGATTTTGTTCA
GACCCGGGAAAGATAATATTTCTTTCAAGATATTACCTGCCGGTGATGTGTTGCAAACCAATATGATCATTGTGGGTGAT
CCGTGTGAGGACGATATTTCTTTTTCGTGGTATGCAATCTGTAAAACAGACAACGGTGACTACATATCCATTGATACCTC
CAAGGAAAGGAATGGAAGATGTTACGATAGTAATTTCGAAGTTCATGGTGTAGTTGGGAGTTGTCCAATAATAGCGTTAA
GTTTTTCTGAGTTGCTTGATGAGCTATATAAAAGTTCGGGGGCTGATATTTTTTGGAAAGCGAAGGATTACGGCGACGCT
TACGCGTGA