Detailed information of immunity protein


Summary

Immunity protein ID IMU38212 experimental
Cognate effector EFF76639 experimental
Related T6SS (Type) T6SS00126 experimental (Type i4b)
Strain Burkholderia cenocepacia AU 1054
Replicon chromosome
Sequence Protein sequence (163 a.a.); Nucleotide sequence (489 bp)
Description The E-I deletion mutant was outcompeted by its parental strain in a T6SS-dependent manner. Ectopic expression of the cognate immunity gene in each mutant rescued it from T6SS-mediated killing by the parental strain, providing evidence that Tle1-Tli1, Tne1-Tni1, Tne2-Tni2, and Tpe1-Tpi1 are true antibacterial E-I pairs associated with the BcAU1054 T6SS.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) BCEN_RS33450 (tni2)
Coordinates (Strand) 437845..438333 (+)
NCBI ID WP_011547151.1
RefSeq NC_008061
Uniprot ID UPI000052D5A9
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-162 MRSIKEIVEIIRSDGRCQVLPASGKLPQLPHADMAYPNDMVEFYNLCGGVILFRPGKDNISFKILPAGDVLQTNMIIVGDPCEDDISFSWYAICKTDNGDYISIDTSKERNGRCYDSNFEVHGVVGSCPIIALSFSELLDELYKSSGADIFWKAKDYGDAYA



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9622 - -



  Protein sequence: 163 a.a.    .

>IMU38212 NC_008061:437845-438333 [Burkholderia cenocepacia AU 1054]
MRSIKEIVEIIRSDGRCQVLPASGKLPQLPHADMAYPNDMVEFYNLCGGVILFRPGKDNISFKILPAGDVLQTNMIIVGD
PCEDDISFSWYAICKTDNGDYISIDTSKERNGRCYDSNFEVHGVVGSCPIIALSFSELLDELYKSSGADIFWKAKDYGDA
YA*

  Nucleotide sequence: 489 bp    .

>IMU38212 NC_008061:437845-438333 [Burkholderia cenocepacia AU 1054]
ATGAGGTCGATTAAGGAAATTGTCGAGATCATAAGATCCGATGGTAGATGCCAAGTGTTGCCGGCGAGCGGAAAGCTACC
GCAGCTGCCACATGCCGACATGGCATATCCGAATGATATGGTCGAATTCTACAATCTTTGCGGTGGTGTGATTTTGTTCA
GACCCGGGAAAGATAATATTTCTTTCAAGATATTACCTGCCGGTGATGTGTTGCAAACCAATATGATCATTGTGGGTGAT
CCGTGTGAGGACGATATTTCTTTTTCGTGGTATGCAATCTGTAAAACAGACAACGGTGACTACATATCCATTGATACCTC
CAAGGAAAGGAATGGAAGATGTTACGATAGTAATTTCGAAGTTCATGGTGTAGTTGGGAGTTGTCCAATAATAGCGTTAA
GTTTTTCTGAGTTGCTTGATGAGCTATATAAAAGTTCGGGGGCTGATATTTTTTGGAAAGCGAAGGATTACGGCGACGCT
TACGCGTGA