Detailed information of component protein


Summary

Component ID T6CP001216
Component protein type TssM
T6SS ID (Type) T6SS00126 experimental (Type i4b)
Strain Burkholderia cenocepacia AU 1054
Replicon chromosome I
Sequence Protein sequence (1315 a.a.); Nucleotide sequence (3945 bp)
Reference
[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[2] Perault AI, Chandler CE, Rasko DA, et al. Host Adaptation Predisposes Pseudomonas aeruginosa to Type VI Secretion System-Mediated Predation by the Burkholderia cepacia Complex. Cell Host Microbe. 2020 Oct 7;28(4):534-547.e3. doi: 10.1016/j.chom.2020.06.019. Epub 2020 Aug 4. PMID: 32755549
[3] Loeven NA, Perault AI, Cotter PA, et al. The Burkholderia cenocepacia Type VI Secretion System Effector TecA Is a Virulence Factor in Mouse Models of Lung Infection. mBio. 2021 Oct 26;12(5):e0209821. doi: 10.1128/mBio.02098-21. Epub 2021 Sep 28. PMID: 34579569
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BCEN_RS13060
Coordinate (Strand) 2880426..2884370 (+)
NCBI ID WP_011546567.1
RefSeq NC_008060
Uniprot ID A0A0H2XS99_BURCA
KEGG ID bcn:Bcen_2620
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 5.2e-95 498..877
IcmF-related_N PF14331.8 9.8e-83 194..447
IcmF_C PF06744.14 7.7e-22 1147..1250
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MQRILNVLTHPR
Transmembrane helix 13-30 TLSIVGIVALAAILFIVA
Outside 31-34 DMLQ
Transmembrane helix 35-57 LPLLWAALAFAAVLALWLVVALW
Inside 58-443 RRWRVKRANQQLGQVLEEQAETGKIAAPAAAALAPDAKTADLDVLRTRLSDAVKTIKTSKIGQVSGGSALYELPWYIVIGNPAAGKSSAVLNSGLQFPFADKNSAVIHGIGGTRNCDWFFTTEGILLDTAGRYSVHEEDRSEWLGFLGLLKRYRPKAPINGIIVTASIAELTGNRPEFAINLAKNLRQRVQELTEKLEVFAPVYVMFTKADLITGFTEFFSSSDKHEYDRVWGATLPYEPDDKRDVVAQFDTHFEELYEGLKEISVAQLSLSRGNQLSPGQLSFPLEFSTIKPSLRAFLATLFENNPFQYKPIFRGFYFTSALQEGETNSAAAQRIAHRFGLDGSALPKPHSAFSKNGFFLRDLFSKVIFADRQTVRQFASPTKTR
Transmembrane helix 444-466 LRYATFFGFVAALALALGGWTWS
Outside 467-1314 TIGNQQLVANVQADLDNVTRLQQGRNDLQSRLQAMDIIEDRIEQLEQFRRDKPLSVSLGLYQGDRLEQHLLTEYYNGVRQILLAPVSQNLASFMKDVNAHPEQLVPMTRPPESGAVQGGTLPVSTNAAGAAPQAGAMLAASGTAPAAAPQQAAAPQGGLYSDASPTNVQDAYNALKTYLMLSDKRHVEQAHLTDQLARFWRGWLETNRGNMPRDEMIRSAERMISFYLSRVNDDDWPMIDANLALVDQTRENLRRVVRGMPARQRVYEEIKARASTRFAPMTIARIVGDGNQGLVAGSYAIPGTFTREAWFDYVQPAIRDAATKELQAKDWVLNTSTQDDLTLEGSPEQIQKTLVGMYKTEYAQHWQKFMQGIAVQGFTSFGQAVDGMNRLGDPQDSPIRKILETAYDQTSWDNPSLANVTIKKAQTGVVNWVKQLFTRSQAGQMAAANIDINGNPAEVPMGPIGQEFVGLARIVATHDGTSMLKGYMESLSKVRTRFNVIKNQGDPGPGARQLMQQTLDGNGSELADSLKLVDEQMLTGLTDSQRKSLRPLLVRPLMQAFAVVIQPASAEVNKVWNAQVYQPFQSSLATKYPFAANAKVEAGAGEIAQVFGPDGSIAKFVGTTLGPLAVRRGDTLAARTWGDMGIGLTPDFTNGFARWVAPLAGGAAGSAAASAEPQTVFQILPQPSTGTTEYTIAIDGQQLRYRNTPPQWTNFVWPNPQGAPGATLSATTFDGRTVQLVNEPGRYGLEKLINSAQRKRRPDGTFDLTWAQGSVNVSVTMRIISTSQPSAGGGDQPQQQSLRGLQLPSSVADASAGAAQNATQAGTGTPGAAPAVAAANATNAQRAQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9974 - -


  Protein sequence: 1315 a.a.    

>T6CP001216 NC_008060:2880426-2884370 [Burkholderia cenocepacia AU 1054] [TssM]
MQRILNVLTHPRTLSIVGIVALAAILFIVADMLQLPLLWAALAFAAVLALWLVVALWRRWRVKRANQQLGQVLEEQAETG
KIAAPAAAALAPDAKTADLDVLRTRLSDAVKTIKTSKIGQVSGGSALYELPWYIVIGNPAAGKSSAVLNSGLQFPFADKN
SAVIHGIGGTRNCDWFFTTEGILLDTAGRYSVHEEDRSEWLGFLGLLKRYRPKAPINGIIVTASIAELTGNRPEFAINLA
KNLRQRVQELTEKLEVFAPVYVMFTKADLITGFTEFFSSSDKHEYDRVWGATLPYEPDDKRDVVAQFDTHFEELYEGLKE
ISVAQLSLSRGNQLSPGQLSFPLEFSTIKPSLRAFLATLFENNPFQYKPIFRGFYFTSALQEGETNSAAAQRIAHRFGLD
GSALPKPHSAFSKNGFFLRDLFSKVIFADRQTVRQFASPTKTRLRYATFFGFVAALALALGGWTWSTIGNQQLVANVQAD
LDNVTRLQQGRNDLQSRLQAMDIIEDRIEQLEQFRRDKPLSVSLGLYQGDRLEQHLLTEYYNGVRQILLAPVSQNLASFM
KDVNAHPEQLVPMTRPPESGAVQGGTLPVSTNAAGAAPQAGAMLAASGTAPAAAPQQAAAPQGGLYSDASPTNVQDAYNA
LKTYLMLSDKRHVEQAHLTDQLARFWRGWLETNRGNMPRDEMIRSAERMISFYLSRVNDDDWPMIDANLALVDQTRENLR
RVVRGMPARQRVYEEIKARASTRFAPMTIARIVGDGNQGLVAGSYAIPGTFTREAWFDYVQPAIRDAATKELQAKDWVLN
TSTQDDLTLEGSPEQIQKTLVGMYKTEYAQHWQKFMQGIAVQGFTSFGQAVDGMNRLGDPQDSPIRKILETAYDQTSWDN
PSLANVTIKKAQTGVVNWVKQLFTRSQAGQMAAANIDINGNPAEVPMGPIGQEFVGLARIVATHDGTSMLKGYMESLSKV
RTRFNVIKNQGDPGPGARQLMQQTLDGNGSELADSLKLVDEQMLTGLTDSQRKSLRPLLVRPLMQAFAVVIQPASAEVNK
VWNAQVYQPFQSSLATKYPFAANAKVEAGAGEIAQVFGPDGSIAKFVGTTLGPLAVRRGDTLAARTWGDMGIGLTPDFTN
GFARWVAPLAGGAAGSAAASAEPQTVFQILPQPSTGTTEYTIAIDGQQLRYRNTPPQWTNFVWPNPQGAPGATLSATTFD
GRTVQLVNEPGRYGLEKLINSAQRKRRPDGTFDLTWAQGSVNVSVTMRIISTSQPSAGGGDQPQQQSLRGLQLPSSVADA
SAGAAQNATQAGTGTPGAAPAVAAANATNAQRAQ*

  Nucleotide sequence: 3945 bp    

>T6CP001216 NC_008060:2880426-2884370 [Burkholderia cenocepacia AU 1054] [TssM]
ATGCAACGCATCCTCAACGTGCTGACTCATCCGCGTACGCTCTCGATCGTCGGGATCGTCGCGCTAGCGGCCATTCTGTT
CATCGTCGCCGACATGCTGCAGCTGCCGCTCCTGTGGGCGGCGCTCGCGTTCGCGGCGGTCCTCGCGCTGTGGCTCGTCG
TCGCGCTATGGCGCCGCTGGCGCGTGAAGCGCGCGAACCAGCAGCTCGGCCAGGTGCTGGAAGAGCAGGCGGAAACCGGC
AAGATCGCCGCACCGGCCGCCGCCGCGCTCGCGCCGGACGCGAAGACGGCCGACCTCGACGTGCTGCGCACGCGCCTGTC
GGATGCGGTGAAGACGATCAAGACGTCGAAGATCGGCCAGGTGTCGGGCGGCTCCGCGCTGTACGAACTGCCGTGGTACA
TCGTGATCGGCAACCCAGCCGCGGGCAAGAGCAGCGCCGTGCTCAATTCCGGCCTGCAGTTCCCATTCGCGGACAAGAAC
AGCGCCGTGATCCACGGCATCGGCGGCACGCGTAACTGCGACTGGTTCTTCACGACCGAAGGGATCCTGCTCGATACGGC
CGGCCGCTATTCGGTGCACGAGGAAGACCGTAGCGAGTGGCTCGGCTTCCTCGGGCTGCTCAAGCGCTACCGTCCGAAGG
CGCCGATCAACGGCATCATCGTCACGGCCAGCATCGCCGAACTCACCGGCAATCGCCCCGAATTCGCGATCAACCTCGCG
AAGAACCTCCGTCAGCGCGTTCAGGAGCTGACCGAAAAGCTGGAAGTGTTCGCGCCGGTCTACGTGATGTTCACGAAGGC
CGACCTGATCACCGGCTTCACCGAATTCTTCAGCAGCAGCGACAAGCACGAGTACGACCGCGTGTGGGGCGCCACCCTGC
CCTACGAACCCGACGACAAGCGTGACGTCGTCGCGCAGTTCGACACGCACTTCGAGGAACTCTACGAAGGGCTGAAGGAA
ATCAGCGTCGCGCAGCTGTCGCTGTCGCGCGGCAACCAGCTGTCGCCGGGCCAGTTGAGCTTTCCGCTCGAATTCTCGAC
GATCAAGCCGTCGCTGCGCGCGTTCCTCGCGACGCTGTTCGAGAACAACCCGTTCCAGTACAAGCCGATCTTCCGCGGCT
TCTACTTCACCAGCGCACTGCAGGAAGGCGAAACCAACAGCGCGGCCGCGCAGCGCATCGCGCACCGCTTCGGCCTCGAC
GGCAGCGCGCTGCCGAAACCGCACAGCGCGTTCTCGAAGAACGGCTTCTTCCTGCGCGACCTGTTCTCGAAGGTGATCTT
CGCGGACCGCCAGACGGTGCGCCAGTTCGCGAGCCCGACCAAGACGCGGCTGCGCTACGCGACCTTCTTCGGCTTCGTCG
CGGCGCTCGCGCTCGCGCTGGGCGGCTGGACCTGGTCGACCATCGGCAACCAGCAGCTCGTCGCGAACGTGCAGGCCGAC
CTCGACAACGTCACGCGCCTGCAGCAGGGCCGCAACGACCTGCAGTCGCGCCTGCAGGCGATGGACATCATCGAGGACCG
GATCGAGCAGCTCGAACAGTTCCGCCGCGACAAGCCGCTGTCGGTGTCGCTCGGCCTGTACCAGGGCGACCGTCTCGAGC
AGCACCTGCTGACCGAGTACTACAACGGCGTGCGCCAGATCCTGCTGGCCCCGGTGTCGCAGAACCTCGCGTCGTTCATG
AAGGACGTGAACGCGCACCCCGAGCAGCTCGTGCCGATGACGCGCCCGCCTGAATCGGGCGCCGTGCAGGGCGGCACGCT
GCCGGTCTCGACGAACGCGGCAGGAGCCGCGCCGCAAGCGGGCGCCATGCTGGCCGCGTCCGGCACCGCGCCGGCCGCCG
CCCCGCAACAGGCGGCCGCGCCGCAGGGCGGCCTCTACAGCGACGCGTCGCCGACCAACGTGCAGGACGCGTACAACGCG
CTGAAGACCTACCTGATGCTGTCCGACAAGCGTCACGTCGAACAGGCGCACCTGACCGACCAGCTCGCCCGCTTCTGGCG
CGGCTGGCTCGAGACGAATCGCGGCAACATGCCGCGCGACGAGATGATCCGGAGCGCCGAGCGCATGATCTCGTTCTACC
TGTCGCGCGTGAACGACGACGACTGGCCGATGATCGACGCGAACCTCGCGCTCGTCGACCAGACCCGCGAGAACCTGCGC
CGCGTCGTGCGCGGCATGCCGGCCCGCCAGCGCGTCTACGAGGAAATCAAGGCCCGCGCGTCGACCCGCTTCGCGCCGAT
GACCATCGCGCGCATCGTCGGCGACGGCAACCAGGGGCTCGTGGCAGGCAGCTATGCGATTCCGGGCACGTTCACGCGCG
AAGCGTGGTTCGACTACGTGCAGCCGGCGATCCGCGATGCGGCGACCAAGGAGCTGCAGGCGAAGGACTGGGTGCTCAAC
ACGTCGACGCAGGACGACCTGACGCTCGAGGGCAGCCCCGAGCAGATCCAGAAGACGCTCGTCGGCATGTACAAGACCGA
GTACGCGCAGCACTGGCAGAAGTTCATGCAGGGCATCGCGGTACAGGGCTTCACCAGCTTCGGCCAGGCCGTCGACGGGA
TGAACCGCCTCGGCGACCCGCAGGATTCGCCGATCCGCAAGATCCTCGAGACCGCGTACGACCAGACGTCGTGGGACAAC
CCGTCGCTCGCGAACGTGACGATCAAGAAGGCGCAGACGGGCGTCGTGAACTGGGTCAAGCAACTGTTCACGCGCTCGCA
GGCCGGCCAGATGGCGGCAGCCAACATCGACATCAACGGCAATCCGGCCGAGGTGCCGATGGGTCCGATCGGCCAGGAAT
TCGTCGGACTCGCGCGGATCGTCGCGACGCATGACGGCACGTCGATGCTCAAGGGCTACATGGAGTCGCTGTCGAAGGTC
CGCACCCGCTTCAACGTGATCAAGAACCAGGGCGACCCGGGGCCGGGCGCGCGCCAGCTGATGCAGCAGACGCTCGACGG
CAACGGTTCGGAACTCGCCGATTCGCTGAAGCTCGTCGACGAGCAGATGTTGACGGGCCTGACCGATTCGCAACGCAAGT
CGCTGCGCCCGCTGCTGGTGCGGCCGCTGATGCAGGCGTTCGCGGTCGTGATCCAGCCGGCCAGCGCCGAAGTCAACAAG
GTGTGGAACGCGCAGGTCTACCAGCCGTTCCAGAGCTCGCTCGCGACCAAGTACCCGTTCGCGGCGAACGCGAAGGTCGA
GGCCGGCGCCGGCGAGATCGCGCAGGTGTTCGGTCCGGACGGCTCGATCGCGAAGTTCGTCGGCACGACGCTCGGGCCGC
TCGCGGTGCGCCGCGGCGACACGCTCGCCGCCCGCACGTGGGGCGACATGGGCATCGGGCTCACGCCGGACTTCACGAAC
GGCTTCGCACGCTGGGTCGCGCCGCTCGCGGGCGGCGCCGCCGGCAGCGCGGCCGCGTCGGCCGAACCGCAGACCGTGTT
CCAGATCCTGCCGCAACCGAGCACGGGCACGACGGAATACACGATCGCGATCGACGGCCAGCAGCTGCGCTACCGCAACA
CGCCGCCGCAGTGGACCAACTTCGTGTGGCCAAACCCGCAGGGCGCGCCGGGCGCGACGCTGTCCGCGACGACCTTCGAC
GGCCGCACGGTGCAGCTCGTCAACGAGCCGGGCCGCTACGGTCTCGAGAAGCTGATCAACTCGGCGCAGCGCAAGCGCCG
TCCGGACGGCACCTTCGATCTGACGTGGGCACAAGGCAGCGTGAACGTGTCGGTGACGATGCGCATCATCAGCACGTCGC
AACCGTCGGCCGGTGGCGGCGACCAGCCGCAGCAGCAGAGCCTGCGCGGCCTGCAGCTGCCGTCGTCGGTCGCCGACGCG
AGCGCGGGCGCCGCGCAGAACGCGACGCAAGCCGGCACCGGCACGCCGGGCGCCGCGCCGGCCGTCGCGGCCGCCAACGC
AACGAATGCACAGAGGGCGCAATGA