Detailed information of immunity protein


Summary

Immunity protein ID IMU38214 experimental
Cognate effector EFF76641 experimental
Related T6SS (Type) T6SS00126 experimental (Type i4b)
Strain Burkholderia cenocepacia AU 1054
Replicon chromosome
Sequence Protein sequence (521 a.a.); Nucleotide sequence (1563 bp)
Description The E-I deletion mutant was outcompeted by its parental strain in a T6SS-dependent manner. Ectopic expression of the cognate immunity gene in each mutant rescued it from T6SS-mediated killing by the parental strain, providing evidence that Tle1-Tli1, Tne1-Tni1, Tne2-Tni2, and Tpe1-Tpi1 are true antibacterial E-I pairs associated with the BcAU1054 T6SS.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) BCEN_RS06975 (tli2)
Coordinates (Strand) 1551205..1552767 (-)
NCBI ID WP_011545610.1
RefSeq NC_008060
Uniprot ID A0A0H2XNQ4_BURCA, A0A427NSL2_9BURK
KEGG ID bcn:Bcen_1409
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DUF6396 PF19933.1 2.2e-11 242..291
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-520 MNDSLPRNMSLTAFDPHRTAFECKHEVDAVPPLDPEADRWNQQAMALTSSLLWPNQRDYKGAVDLWTKAAERKHWKAMLNLANAYAQGQGVERDSEHAVQITEQAMKLGIPAAYDLMGTYHMNGMGVKQDASRAYAFWQLAADMGSPSAMAYLGGKMDALYDDPKAGFWGNRKIALKMLECAVAQGNGLAAYELGTTIVGDRAELSENNERALKTLHAGVKFGSAESAGWLSASFGKGDALVGHVKDEARAERYSVLADRLERDPDLRLPNLDKVVPLPPAKLPKWDGNKETLIDAAKAVTSVPASPVKPAAHPVSLRTGRAHVPDGYMLPEKPQVAVPPQAETTAAPVGGYWLAQLKYPSAERHFAWNAAQVPMHYRNDELFDRSRPGLMPEDGRIFFHYVGDAIPMPVQSAESHPRVTQGIARAAEFPDPAIRCRGIRACPVTGIWQAVVADDHPRATTFNQWYRQAYVLQGDAFPDPRVMHLDVSPADVTWTWWNEANHLGFAKLPQVSLGNPSENA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9849 - -


  Protein sequence: 521 a.a.    .

>IMU38214 NC_008060:c1552767-1551205 [Burkholderia cenocepacia AU 1054]
MNDSLPRNMSLTAFDPHRTAFECKHEVDAVPPLDPEADRWNQQAMALTSSLLWPNQRDYKGAVDLWTKAAERKHWKAMLN
LANAYAQGQGVERDSEHAVQITEQAMKLGIPAAYDLMGTYHMNGMGVKQDASRAYAFWQLAADMGSPSAMAYLGGKMDAL
YDDPKAGFWGNRKIALKMLECAVAQGNGLAAYELGTTIVGDRAELSENNERALKTLHAGVKFGSAESAGWLSASFGKGDA
LVGHVKDEARAERYSVLADRLERDPDLRLPNLDKVVPLPPAKLPKWDGNKETLIDAAKAVTSVPASPVKPAAHPVSLRTG
RAHVPDGYMLPEKPQVAVPPQAETTAAPVGGYWLAQLKYPSAERHFAWNAAQVPMHYRNDELFDRSRPGLMPEDGRIFFH
YVGDAIPMPVQSAESHPRVTQGIARAAEFPDPAIRCRGIRACPVTGIWQAVVADDHPRATTFNQWYRQAYVLQGDAFPDP
RVMHLDVSPADVTWTWWNEANHLGFAKLPQVSLGNPSENA*

  Nucleotide sequence: 1563 bp    .

>IMU38214 NC_008060:c1552767-1551205 [Burkholderia cenocepacia AU 1054]
ATGAACGATTCTCTTCCTCGCAACATGTCGCTGACCGCCTTCGATCCGCATCGCACAGCATTCGAGTGCAAGCACGAGGT
TGACGCGGTTCCTCCGCTGGATCCGGAGGCCGATCGCTGGAATCAGCAGGCAATGGCGCTGACCAGTTCGCTGTTGTGGC
CGAACCAGCGCGATTACAAGGGGGCTGTCGATTTGTGGACGAAGGCGGCCGAGCGTAAGCACTGGAAAGCCATGCTGAAT
CTGGCGAATGCCTACGCTCAGGGGCAAGGTGTGGAACGTGATTCGGAACATGCCGTTCAGATCACCGAACAGGCGATGAA
ACTGGGTATTCCGGCAGCCTACGATTTGATGGGCACGTACCACATGAACGGGATGGGGGTTAAGCAGGATGCGAGCCGCG
CCTATGCGTTCTGGCAACTGGCCGCCGATATGGGTAGTCCAAGTGCGATGGCGTATCTGGGTGGCAAGATGGATGCGCTC
TATGACGACCCGAAGGCAGGCTTCTGGGGTAACCGAAAGATCGCGCTCAAGATGCTTGAATGTGCGGTCGCGCAGGGCAA
CGGACTTGCTGCATATGAGTTGGGAACGACGATTGTGGGAGATCGAGCCGAGTTGAGCGAGAACAACGAACGCGCACTCA
AGACCCTGCATGCGGGCGTGAAATTCGGCAGCGCCGAGAGCGCGGGGTGGTTGAGTGCGTCGTTTGGCAAGGGGGACGCC
CTGGTCGGCCATGTCAAGGATGAAGCCCGCGCCGAACGCTACAGCGTCCTTGCCGACCGCCTCGAGCGCGACCCCGACCT
GCGTCTGCCGAATCTGGACAAGGTCGTGCCGCTGCCGCCCGCCAAGTTGCCCAAGTGGGACGGCAACAAGGAGACGTTGA
TCGACGCGGCGAAAGCGGTGACGTCCGTGCCGGCGTCGCCGGTCAAACCTGCGGCGCACCCCGTGTCGCTGCGTACGGGG
CGCGCGCACGTGCCCGATGGCTACATGCTGCCGGAGAAGCCGCAGGTTGCCGTTCCTCCGCAGGCGGAAACGACGGCTGC
TCCTGTCGGTGGTTACTGGCTCGCCCAGCTGAAGTATCCGAGCGCCGAACGTCACTTCGCATGGAATGCGGCTCAGGTAC
CGATGCATTACCGGAATGATGAACTGTTCGATCGGAGTCGCCCCGGCCTGATGCCTGAGGACGGTCGCATCTTCTTCCAC
TATGTCGGCGATGCGATTCCGATGCCCGTGCAATCGGCGGAATCGCATCCGCGTGTGACACAGGGCATTGCGCGCGCCGC
CGAATTTCCCGACCCGGCTATCCGGTGTCGCGGCATCCGCGCGTGCCCGGTGACCGGTATTTGGCAGGCTGTGGTGGCGG
ACGATCATCCTCGGGCGACCACGTTCAATCAGTGGTATCGCCAGGCCTACGTGTTGCAGGGTGACGCGTTTCCCGATCCA
CGGGTCATGCATCTGGACGTATCGCCGGCGGACGTGACGTGGACGTGGTGGAACGAAGCTAATCACCTTGGATTTGCGAA
GCTGCCTCAGGTCAGCCTCGGCAACCCTTCCGAGAACGCTTGA