Detailed information of effector


Summary

Effector ID EFF76641 experimental
Effector type Tle
Cognate immunity protein IMU38214 experimental
Related T6SS (Type) T6SS00126 experimental (Type i4b)
Strain Burkholderia cenocepacia AU 1054
Replicon chromosome
Sequence Protein sequence (694 a.a.); Nucleotide sequence (2082 bp)
Description The E-I deletion mutant was outcompeted by its parental strain in a T6SS-dependent manner. Ectopic expression of the cognate immunity gene in each mutant rescued it from T6SS-mediated killing by the parental strain, providing evidence that Tle1-Tli1, Tne1-Tni1, Tne2-Tni2, and Tpe1-Tpi1 are true antibacterial E-I pairs associated with the BcAU1054 T6SS.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) BCEN_RS06980 (tle2)
Coordinates (Strand) 1552877..1554958 (-)
NCBI ID WP_011545611.1
RefSeq NC_008060
Uniprot ID A0A0H2XPF8_BURCA, A0A427NSJ9_9BURK
KEGG ID bcn:Bcen_1410
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
PLDc_2 PF13091.8 4e-06 581..628
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-693 MSDTNTQNKSKAPIDVDKRQAVASAQWFLEKQADYYAPPYEKNNIDVFICGEDAFTQIAADLKAAKHSVEIICWGFDPGMELIRKGTVWPRGDTWGGLLRDVAAGKFNNGKPVQVRLLSWYGVIGSTLSNNMPGYSGGRLTYEQKMAREVGKAGGIYVQLGNAPQPLTPQDRREDFNARWYDDAFAGRLPNLSLRTRDGSSKDVHESLAHEPGKRDNLEDFGLEKLATDHQKTILIDYDHEGGTQAVGYVMGLNSVTDYWDTQQHLFNDPRRGEGWEGANDSQPGLKPYQDYASRIRGEALVAVSKNFTDAWNRAKGQGANLSRTHDFKKVPPGLTKNIGTPCQRAQIARTQPEEKDKSIKALYIQATSFARNYIYIENQYFQYTEWPTKLKELRTEFLSCSQAAGRSPADVPNLHVMAVIPTPERPQMVPRTHDTVKVLGYGTSMPNQDKAVEDELARNRQQQATWDAYVKRQKASGARIDPDYYPAPLSPAAQSAKDLGDKASLTKSLDQMGIRALVGSLWSFDYNWRSSKLNTETEKKQARWDDYVKKQQAKGELPDPDLAPMLPLPAQSFAARYREIYIHSKLLMIDDSFFTLGSANLNLRSMAVDAEINVGTDDRAKSEDLRKRVWKLHTGNAADCNPSSLTPLAMSKAFRNWVQLLRANELARKKGDAPTGFLHPFHDDRVTGIRLS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9907 - -


  Protein sequence: 694 a.a.    .

>EFF76641 NC_008060:c1554958-1552877 Type VI Lipase Effector (Tle) [Burkholderia cenocepacia AU 1054]
MSDTNTQNKSKAPIDVDKRQAVASAQWFLEKQADYYAPPYEKNNIDVFICGEDAFTQIAADLKAAKHSVEIICWGFDPGM
ELIRKGTVWPRGDTWGGLLRDVAAGKFNNGKPVQVRLLSWYGVIGSTLSNNMPGYSGGRLTYEQKMAREVGKAGGIYVQL
GNAPQPLTPQDRREDFNARWYDDAFAGRLPNLSLRTRDGSSKDVHESLAHEPGKRDNLEDFGLEKLATDHQKTILIDYDH
EGGTQAVGYVMGLNSVTDYWDTQQHLFNDPRRGEGWEGANDSQPGLKPYQDYASRIRGEALVAVSKNFTDAWNRAKGQGA
NLSRTHDFKKVPPGLTKNIGTPCQRAQIARTQPEEKDKSIKALYIQATSFARNYIYIENQYFQYTEWPTKLKELRTEFLS
CSQAAGRSPADVPNLHVMAVIPTPERPQMVPRTHDTVKVLGYGTSMPNQDKAVEDELARNRQQQATWDAYVKRQKASGAR
IDPDYYPAPLSPAAQSAKDLGDKASLTKSLDQMGIRALVGSLWSFDYNWRSSKLNTETEKKQARWDDYVKKQQAKGELPD
PDLAPMLPLPAQSFAARYREIYIHSKLLMIDDSFFTLGSANLNLRSMAVDAEINVGTDDRAKSEDLRKRVWKLHTGNAAD
CNPSSLTPLAMSKAFRNWVQLLRANELARKKGDAPTGFLHPFHDDRVTGIRLS*

  Nucleotide sequence: 2082 bp    .

>EFF76641 NC_008060:c1554958-1552877 Type VI Lipase Effector (Tle) [Burkholderia cenocepacia AU 1054]
ATGTCCGATACCAATACTCAAAACAAATCGAAGGCGCCGATCGATGTCGACAAGCGTCAGGCGGTCGCCAGTGCGCAGTG
GTTTCTGGAAAAGCAGGCGGACTACTACGCGCCGCCTTATGAAAAGAACAATATCGACGTATTCATTTGCGGAGAGGACG
CGTTTACGCAGATTGCGGCCGACTTGAAGGCGGCCAAGCATAGTGTCGAAATCATTTGCTGGGGCTTTGACCCCGGGATG
GAACTGATCCGCAAGGGCACGGTCTGGCCGCGAGGAGATACCTGGGGCGGGCTGCTGCGCGATGTCGCGGCCGGGAAATT
CAATAACGGCAAGCCGGTGCAGGTTCGCTTGCTCTCGTGGTATGGCGTGATCGGAAGTACCCTGTCGAACAACATGCCGG
GCTACAGCGGCGGCCGCCTTACGTACGAGCAGAAGATGGCCAGGGAGGTCGGCAAGGCGGGCGGCATCTATGTGCAGTTG
GGCAATGCGCCGCAGCCGCTGACCCCACAGGACCGGCGCGAGGACTTCAATGCGCGATGGTACGACGACGCGTTTGCCGG
CCGCCTGCCCAACCTGTCTCTTCGTACGCGGGACGGCAGCAGCAAGGACGTTCACGAAAGCCTGGCGCACGAGCCGGGCA
AGCGCGACAACCTGGAAGATTTCGGCCTGGAGAAATTGGCCACCGACCATCAGAAGACTATCCTGATCGACTACGACCAC
GAGGGCGGTACACAGGCGGTCGGCTACGTGATGGGGCTGAACAGCGTGACGGACTATTGGGATACGCAGCAGCATCTCTT
CAATGATCCGCGTCGTGGTGAAGGATGGGAGGGTGCCAACGACTCGCAACCGGGGTTGAAGCCTTATCAGGACTACGCGA
GTCGGATTCGGGGCGAAGCACTCGTGGCGGTAAGCAAAAACTTTACCGATGCGTGGAACCGGGCCAAGGGTCAGGGGGCG
AACCTGTCGCGAACACACGATTTCAAGAAGGTGCCTCCGGGTTTGACGAAAAATATCGGCACGCCGTGCCAGCGTGCGCA
AATCGCTCGTACGCAGCCCGAAGAAAAGGACAAATCCATCAAGGCCTTGTATATCCAGGCGACGTCGTTTGCGCGCAACT
ATATCTACATCGAGAACCAGTATTTCCAATATACCGAGTGGCCGACGAAGCTGAAGGAATTGCGAACCGAGTTTCTGTCG
TGCAGCCAGGCAGCGGGAAGGAGTCCTGCCGACGTACCGAATTTGCACGTGATGGCCGTTATCCCCACGCCCGAGCGGCC
ACAGATGGTGCCGCGCACGCACGATACCGTTAAGGTTCTGGGCTATGGCACCTCGATGCCCAATCAGGACAAGGCGGTCG
AAGACGAACTCGCGCGCAATCGCCAGCAACAGGCGACCTGGGACGCCTACGTCAAGCGGCAGAAAGCGAGCGGCGCAAGG
ATCGACCCCGACTACTATCCTGCTCCGCTGTCGCCTGCGGCGCAAAGCGCCAAGGACCTTGGTGACAAGGCGTCGCTCAC
GAAATCGCTCGATCAGATGGGCATCCGTGCGCTGGTCGGCAGCCTGTGGTCCTTTGACTACAATTGGCGCAGCTCCAAGC
TGAATACCGAGACGGAGAAGAAACAGGCCCGCTGGGATGACTACGTAAAAAAGCAGCAAGCCAAAGGCGAACTGCCCGAC
CCGGACTTGGCGCCGATGCTGCCGTTGCCGGCGCAATCGTTCGCGGCCCGGTACAGGGAAATCTACATCCACAGTAAATT
GCTGATGATCGACGACAGTTTCTTCACGTTGGGCAGTGCCAATCTGAACTTGCGGAGCATGGCAGTGGATGCCGAGATCA
ACGTGGGAACCGACGACCGCGCGAAAAGCGAAGATCTGCGCAAACGGGTGTGGAAGCTTCACACAGGAAATGCAGCGGAT
TGCAACCCGTCTTCGCTGACTCCGCTGGCCATGTCGAAGGCGTTCCGCAACTGGGTGCAATTGCTGCGGGCCAACGAGCT
TGCCCGAAAGAAGGGGGACGCGCCAACCGGCTTCCTGCATCCGTTCCACGATGACCGTGTGACAGGTATTCGCTTATCCT
GA