Detailed information of immunity protein


Summary

Immunity protein ID IMU38211 experimental
Cognate effector EFF76638 experimental
Related T6SS (Type) T6SS00126 experimental (Type i4b)
Strain Burkholderia cenocepacia AU 1054
Replicon chromosome
Sequence Protein sequence (192 a.a.); Nucleotide sequence (576 bp)
Description The E-I deletion mutant was outcompeted by its parental strain in a T6SS-dependent manner. Ectopic expression of the cognate immunity gene in each mutant rescued it from T6SS-mediated killing by the parental strain, providing evidence that Tle1-Tli1, Tne1-Tni1, Tne2-Tni2, and Tpe1-Tpi1 are true antibacterial E-I pairs associated with the BcAU1054 T6SS.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) BCEN_RS33190 (tni1)
Coordinates (Strand) 2911341..2911916 (-)
NCBI ID WP_011546584.1
RefSeq NC_008060
Uniprot ID UPI00005356AC
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-191 MTKIWTTEADYPESRMALYDEAKNQVDRFDLQAATRLTKSNPLVFDLPKKGVAKIQDYGVIWSNILIPLVSEAVRVAIEGLANDGDVQFIPAVIESDGKAIDGSYFLLNPVRKVDVIDHDASEPIVVKIPGFPDAKVGFKKMVLRSDFSANGIGRQYDSLTYIVVGDRLAEAVLCATKKGVRFTTGIPPAD



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9929 - -



  Protein sequence: 192 a.a.    .

>IMU38211 NC_008060:c2911916-2911341 [Burkholderia cenocepacia AU 1054]
MTKIWTTEADYPESRMALYDEAKNQVDRFDLQAATRLTKSNPLVFDLPKKGVAKIQDYGVIWSNILIPLVSEAVRVAIEG
LANDGDVQFIPAVIESDGKAIDGSYFLLNPVRKVDVIDHDASEPIVVKIPGFPDAKVGFKKMVLRSDFSANGIGRQYDSL
TYIVVGDRLAEAVLCATKKGVRFTTGIPPAD*

  Nucleotide sequence: 576 bp    .

>IMU38211 NC_008060:c2911916-2911341 [Burkholderia cenocepacia AU 1054]
ATGACGAAAATCTGGACCACGGAGGCGGACTATCCGGAAAGTCGGATGGCACTCTACGATGAGGCAAAAAATCAAGTGGA
TCGCTTTGATTTACAGGCGGCCACACGCCTCACAAAATCGAATCCTTTGGTATTCGATCTTCCCAAAAAGGGGGTGGCAA
AGATTCAAGACTACGGTGTTATTTGGTCAAACATCTTGATTCCGCTTGTAAGTGAAGCGGTGCGTGTTGCGATTGAAGGA
CTGGCGAATGACGGTGACGTACAGTTCATTCCGGCAGTTATTGAATCGGACGGAAAGGCTATTGACGGATCATACTTTCT
TCTTAATCCGGTTAGAAAAGTCGATGTGATCGATCACGACGCTTCTGAGCCGATAGTGGTGAAGATTCCGGGGTTTCCTG
ATGCCAAGGTTGGTTTCAAAAAGATGGTCTTGCGCTCCGATTTCTCTGCCAATGGCATTGGCAGACAATACGACTCCTTG
ACATATATTGTCGTGGGAGATCGATTGGCCGAGGCTGTATTGTGTGCCACAAAGAAGGGGGTTAGATTCACCACTGGTAT
TCCGCCTGCCGATTGA