Detailed information of immunity protein
Summary
| Immunity protein ID | IMU38211  |
| Cognate effector | EFF76638 |
| Related T6SS (Type) | T6SS00126 (Type i4b) |
| Strain | Burkholderia cenocepacia AU 1054 |
| Replicon | chromosome |
| Sequence | Protein sequence (192 a.a.); Nucleotide sequence (576 bp) |
| Description | The E-I deletion mutant was outcompeted by its parental strain in a T6SS-dependent manner. Ectopic expression of the cognate immunity gene in each mutant rescued it from T6SS-mediated killing by the parental strain, providing evidence that Tle1-Tli1, Tne1-Tni1, Tne2-Tni2, and Tpe1-Tpi1 are true antibacterial E-I pairs associated with the BcAU1054 T6SS. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | BCEN_RS33190 (tni1) |
| Coordinates (Strand) | 2911341..2911916 (-) |
| NCBI ID | WP_011546584.1 |
| RefSeq | NC_008060 |
| Uniprot ID | UPI00005356AC |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-191 | MTKIWTTEADYPESRMALYDEAKNQVDRFDLQAATRLTKSNPLVFDLPKKGVAKIQDYGVIWSNILIPLVSEAVRVAIEGLANDGDVQFIPAVIESDGKAIDGSYFLLNPVRKVDVIDHDASEPIVVKIPGFPDAKVGFKKMVLRSDFSANGIGRQYDSLTYIVVGDRLAEAVLCATKKGVRFTTGIPPAD |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9929 | - | - |
Protein sequence: 192 a.a. .
>IMU38211 NC_008060:c2911916-2911341 [Burkholderia cenocepacia AU 1054]
MTKIWTTEADYPESRMALYDEAKNQVDRFDLQAATRLTKSNPLVFDLPKKGVAKIQDYGVIWSNILIPLVSEAVRVAIEG
LANDGDVQFIPAVIESDGKAIDGSYFLLNPVRKVDVIDHDASEPIVVKIPGFPDAKVGFKKMVLRSDFSANGIGRQYDSL
TYIVVGDRLAEAVLCATKKGVRFTTGIPPAD*
MTKIWTTEADYPESRMALYDEAKNQVDRFDLQAATRLTKSNPLVFDLPKKGVAKIQDYGVIWSNILIPLVSEAVRVAIEG
LANDGDVQFIPAVIESDGKAIDGSYFLLNPVRKVDVIDHDASEPIVVKIPGFPDAKVGFKKMVLRSDFSANGIGRQYDSL
TYIVVGDRLAEAVLCATKKGVRFTTGIPPAD*
Nucleotide sequence: 576 bp .
>IMU38211 NC_008060:c2911916-2911341 [Burkholderia cenocepacia AU 1054]
ATGACGAAAATCTGGACCACGGAGGCGGACTATCCGGAAAGTCGGATGGCACTCTACGATGAGGCAAAAAATCAAGTGGA
TCGCTTTGATTTACAGGCGGCCACACGCCTCACAAAATCGAATCCTTTGGTATTCGATCTTCCCAAAAAGGGGGTGGCAA
AGATTCAAGACTACGGTGTTATTTGGTCAAACATCTTGATTCCGCTTGTAAGTGAAGCGGTGCGTGTTGCGATTGAAGGA
CTGGCGAATGACGGTGACGTACAGTTCATTCCGGCAGTTATTGAATCGGACGGAAAGGCTATTGACGGATCATACTTTCT
TCTTAATCCGGTTAGAAAAGTCGATGTGATCGATCACGACGCTTCTGAGCCGATAGTGGTGAAGATTCCGGGGTTTCCTG
ATGCCAAGGTTGGTTTCAAAAAGATGGTCTTGCGCTCCGATTTCTCTGCCAATGGCATTGGCAGACAATACGACTCCTTG
ACATATATTGTCGTGGGAGATCGATTGGCCGAGGCTGTATTGTGTGCCACAAAGAAGGGGGTTAGATTCACCACTGGTAT
TCCGCCTGCCGATTGA
ATGACGAAAATCTGGACCACGGAGGCGGACTATCCGGAAAGTCGGATGGCACTCTACGATGAGGCAAAAAATCAAGTGGA
TCGCTTTGATTTACAGGCGGCCACACGCCTCACAAAATCGAATCCTTTGGTATTCGATCTTCCCAAAAAGGGGGTGGCAA
AGATTCAAGACTACGGTGTTATTTGGTCAAACATCTTGATTCCGCTTGTAAGTGAAGCGGTGCGTGTTGCGATTGAAGGA
CTGGCGAATGACGGTGACGTACAGTTCATTCCGGCAGTTATTGAATCGGACGGAAAGGCTATTGACGGATCATACTTTCT
TCTTAATCCGGTTAGAAAAGTCGATGTGATCGATCACGACGCTTCTGAGCCGATAGTGGTGAAGATTCCGGGGTTTCCTG
ATGCCAAGGTTGGTTTCAAAAAGATGGTCTTGCGCTCCGATTTCTCTGCCAATGGCATTGGCAGACAATACGACTCCTTG
ACATATATTGTCGTGGGAGATCGATTGGCCGAGGCTGTATTGTGTGCCACAAAGAAGGGGGTTAGATTCACCACTGGTAT
TCCGCCTGCCGATTGA