Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   NSQ25_RS11370 Genome accession   NZ_CP151982
Coordinates   2220744..2221037 (-) Length   97 a.a.
NCBI ID   WP_024422977.1    Uniprot ID   A0A0M2EIN6
Organism   Bacillus sp. FSL R5-0512     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2215744..2226037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ25_RS11320 (NSQ25_11320) sinR 2215907..2216242 (+) 336 WP_024422969.1 transcriptional regulator SinR Regulator
  NSQ25_RS11325 (NSQ25_11325) - 2216310..2217110 (-) 801 WP_034280222.1 TasA family protein -
  NSQ25_RS11330 (NSQ25_11330) - 2217168..2217740 (-) 573 WP_034280218.1 signal peptidase I -
  NSQ25_RS11335 (NSQ25_11335) tapA 2217793..2218320 (-) 528 WP_144496218.1 amyloid fiber anchoring/assembly protein TapA -
  NSQ25_RS11340 (NSQ25_11340) - 2218621..2218899 (+) 279 WP_061109527.1 DUF3889 domain-containing protein -
  NSQ25_RS11345 (NSQ25_11345) - 2218934..2219128 (-) 195 WP_024422974.1 YqzE family protein -
  NSQ25_RS11350 (NSQ25_11350) comGG 2219188..2219571 (-) 384 WP_326136240.1 competence type IV pilus minor pilin ComGG -
  NSQ25_RS11355 (NSQ25_11355) comGF 2219568..2220095 (-) 528 WP_260983449.1 competence type IV pilus minor pilin ComGF -
  NSQ25_RS11360 (NSQ25_11360) comGE 2220007..2220321 (-) 315 WP_034621606.1 competence type IV pilus minor pilin ComGE -
  NSQ25_RS11365 (NSQ25_11365) comGD 2220305..2220751 (-) 447 WP_070326501.1 competence type IV pilus minor pilin ComGD -
  NSQ25_RS11370 (NSQ25_11370) comGC 2220744..2221037 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  NSQ25_RS11375 (NSQ25_11375) comGB 2221054..2222094 (-) 1041 WP_034280202.1 competence type IV pilus assembly protein ComGB -
  NSQ25_RS11380 (NSQ25_11380) comGA 2222075..2223145 (-) 1071 WP_041117979.1 competence type IV pilus ATPase ComGA Machinery gene
  NSQ25_RS11385 (NSQ25_11385) - 2223323..2224198 (-) 876 WP_024422979.1 STAS domain-containing protein -
  NSQ25_RS11395 (NSQ25_11395) - 2224422..2224802 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NSQ25_RS11400 (NSQ25_11400) - 2225015..2225257 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  NSQ25_RS11405 (NSQ25_11405) - 2225302..2225940 (-) 639 WP_034280195.1 MBL fold metallo-hydrolase -

Sequence


Protein


Download         Length: 97 a.a.        Molecular weight: 10686.62 Da        Isoelectric Point: 7.3218

>NTDB_id=983314 NSQ25_RS11370 WP_024422977.1 2220744..2221037(-) (comGC) [Bacillus sp. FSL R5-0512]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQAIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGNVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=983314 NSQ25_RS11370 WP_024422977.1 2220744..2221037(-) (comGC) [Bacillus sp. FSL R5-0512]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATAGTCATGCTCGTAATATCCATTCTTTTACTCATTACCATACC
GAATATCACACGTCATCACCAAGCTATTCAGGCAAAGGGCTGTGAAGGGCTTAAATCAATGGTCAGCACACAAGTGATGG
CATATGAATTGGAGCATGATGGGAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGTTATGTCAAAAAGGGGTTAACT
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCAACGTGAAGCATGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0M2EIN6

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67

  comGC Staphylococcus aureus MW2

42.222

92.784

0.392

  comGC Staphylococcus aureus N315

42.222

92.784

0.392


Multiple sequence alignment