Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WKI13_RS20020 Genome accession   NZ_CP149817
Coordinates   5000869..5001903 (-) Length   344 a.a.
NCBI ID   WP_015819744.1    Uniprot ID   C5BLK3
Organism   Teredinibacter turnerae strain T7902     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4995869..5006903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI13_RS19990 (WKI13_19990) pilG 4996064..4996459 (-) 396 WP_015818072.1 twitching motility response regulator PilG Regulator
  WKI13_RS19995 (WKI13_19995) gshB 4996706..4997665 (+) 960 WP_018275007.1 glutathione synthase -
  WKI13_RS20000 (WKI13_20000) - 4997665..4998546 (+) 882 WP_018275008.1 energy transducer TonB -
  WKI13_RS20005 (WKI13_20005) - 4998630..4999208 (+) 579 WP_018275009.1 YqgE/AlgH family protein -
  WKI13_RS20010 (WKI13_20010) ruvX 4999201..4999641 (+) 441 WP_018275010.1 Holliday junction resolvase RuvX -
  WKI13_RS20015 (WKI13_20015) pilU 4999678..5000820 (-) 1143 WP_018275011.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WKI13_RS20020 (WKI13_20020) pilT 5000869..5001903 (-) 1035 WP_015819744.1 type IV pilus twitching motility protein PilT Machinery gene
  WKI13_RS20025 (WKI13_20025) - 5001949..5002635 (+) 687 WP_018275012.1 YggS family pyridoxal phosphate-dependent enzyme -
  WKI13_RS20030 (WKI13_20030) proC 5002686..5003519 (+) 834 WP_018275013.1 pyrroline-5-carboxylate reductase -
  WKI13_RS20035 (WKI13_20035) - 5003592..5004179 (+) 588 WP_018275014.1 YggT family protein -
  WKI13_RS20040 (WKI13_20040) - 5004242..5004541 (+) 300 WP_018275015.1 DUF167 family protein -
  WKI13_RS20045 (WKI13_20045) - 5004550..5005701 (+) 1152 WP_018275016.1 homoserine O-acetyltransferase -
  WKI13_RS20050 (WKI13_20050) metW 5005701..5006300 (+) 600 WP_018275017.1 methionine biosynthesis protein MetW -
  WKI13_RS20055 (WKI13_20055) - 5006297..5006734 (+) 438 WP_018275018.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38215.93 Da        Isoelectric Point: 6.5790

>NTDB_id=961675 WKI13_RS20020 WP_015819744.1 5000869..5001903(-) (pilT) [Teredinibacter turnerae strain T7902]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGQVFRDVSSVPRGLVLVTGPTGSGKSTTLAAMMDYINENKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPANEKAMVRSMLSESLEAVVSQTLMKKNGGGRVAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGGAIGMQTMDQCL
ADLVERRIISREVAREKAKMPDAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=961675 WKI13_RS20020 WP_015819744.1 5000869..5001903(-) (pilT) [Teredinibacter turnerae strain T7902]
ATGGATATTACCGAATTATTGGCGTTCTCAGCGAAGCAGGGCGCATCGGATTTACACCTCTCTGCTGGCTTGCCGCCAAT
GATCCGCGTCGATGGCGATGTGCGTCGTATCAACCTGCCGCCGATGGAGCACAAGCAGGTACACGGCCTGATCTACGAAA
TTATGAACGACAAGCAACGCAAGGACTACGAGGAGTTCCTGGAAACGGATTTCTCCTTCGAAGTACCGGGCGTGGCGCGT
TTCCGGGTAAACGCATTTAACCAGAATCGTGGTGCAGGCGCGGTATTCCGAACGATTCCCTCCAAAGTGTTGACGATGGA
GGAGCTGGGCATGGGGCAGGTTTTCCGCGATGTGTCCTCGGTGCCACGCGGGCTGGTCCTGGTGACCGGGCCGACCGGTT
CCGGTAAATCTACCACGCTTGCCGCAATGATGGACTACATCAACGAAAACAAATATCACCATATTCTGACTATCGAAGAC
CCAATAGAATTCGTACACGAAAGTAAAAAGTGCCTGGTAAACCAGCGTGAAGTTCATCGCGACACACACGGCTTTGCCGA
GGCGTTGCGCTCCGCATTGCGGGAAGACCCGGACATTATTCTGGTGGGCGAGATGCGGGACCTCGAGACCATCCGGTTGG
CATTAACTGCCGCGGAAACCGGTCACCTGGTGTTCGGTACGCTGCACACCACCTCGGCGGCAAAAACCATCGACCGGGTT
GTCGACGTGTTCCCCGCCAATGAAAAAGCCATGGTCCGCTCAATGTTGTCGGAATCCCTGGAGGCGGTGGTATCGCAAAC
GCTGATGAAAAAAAATGGCGGCGGACGTGTCGCTGCACATGAAATCATGCGCGGTACACCGGCCATTCGCAACCTGATTC
GCGAAGATAAGGTTGCGCAAATGTACTCTGCTATTCAAACCGGCGGCGCCATCGGCATGCAAACGATGGATCAATGTCTG
GCGGATTTGGTGGAACGCCGCATTATTTCGCGTGAAGTCGCTCGCGAAAAAGCTAAGATGCCGGACGCTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C5BLK3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

89.826

100

0.898
  pilT Pseudomonas stutzeri DSM 10701

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

83.14

100

0.831
  pilT Acinetobacter baumannii D1279779

83.14

100

0.831
  pilT Acinetobacter baumannii strain A118

83.14

100

0.831
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Neisseria meningitidis 8013

68.529

98.837

0.677
  pilT Neisseria gonorrhoeae MS11

67.941

98.837

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.647

98.837

0.669
  pilT Vibrio cholerae strain A1552

67.647

98.837

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

40.118

98.547

0.395
  pilU Acinetobacter baylyi ADP1

38.081

100

0.381