Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WKI13_RS20015 Genome accession   NZ_CP149817
Coordinates   4999678..5000820 (-) Length   380 a.a.
NCBI ID   WP_018275011.1    Uniprot ID   -
Organism   Teredinibacter turnerae strain T7902     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4994678..5005820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI13_RS19980 (WKI13_19980) - 4995072..4995611 (-) 540 WP_019603724.1 chemotaxis protein CheW -
  WKI13_RS19985 (WKI13_19985) pilH 4995656..4996018 (-) 363 WP_015818447.1 twitching motility response regulator PilH -
  WKI13_RS19990 (WKI13_19990) pilG 4996064..4996459 (-) 396 WP_015818072.1 twitching motility response regulator PilG Regulator
  WKI13_RS19995 (WKI13_19995) gshB 4996706..4997665 (+) 960 WP_018275007.1 glutathione synthase -
  WKI13_RS20000 (WKI13_20000) - 4997665..4998546 (+) 882 WP_018275008.1 energy transducer TonB -
  WKI13_RS20005 (WKI13_20005) - 4998630..4999208 (+) 579 WP_018275009.1 YqgE/AlgH family protein -
  WKI13_RS20010 (WKI13_20010) ruvX 4999201..4999641 (+) 441 WP_018275010.1 Holliday junction resolvase RuvX -
  WKI13_RS20015 (WKI13_20015) pilU 4999678..5000820 (-) 1143 WP_018275011.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WKI13_RS20020 (WKI13_20020) pilT 5000869..5001903 (-) 1035 WP_015819744.1 type IV pilus twitching motility protein PilT Machinery gene
  WKI13_RS20025 (WKI13_20025) - 5001949..5002635 (+) 687 WP_018275012.1 YggS family pyridoxal phosphate-dependent enzyme -
  WKI13_RS20030 (WKI13_20030) proC 5002686..5003519 (+) 834 WP_018275013.1 pyrroline-5-carboxylate reductase -
  WKI13_RS20035 (WKI13_20035) - 5003592..5004179 (+) 588 WP_018275014.1 YggT family protein -
  WKI13_RS20040 (WKI13_20040) - 5004242..5004541 (+) 300 WP_018275015.1 DUF167 family protein -
  WKI13_RS20045 (WKI13_20045) - 5004550..5005701 (+) 1152 WP_018275016.1 homoserine O-acetyltransferase -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42472.60 Da        Isoelectric Point: 6.3915

>NTDB_id=961674 WKI13_RS20015 WP_018275011.1 4999678..5000820(-) (pilU) [Teredinibacter turnerae strain T7902]
MDFDRLLALMVEKGASDLFITAGVPPSMKLHGKVVPVSSTPLSPEKSRETVLSVMNEKQRKEFLESKELNFAISARGVGR
FRASAFYQRNLAGMVLRRIETQIPKIDDLGLPETIKELAMVKRGLVLFVGATGTGKSTSLAAMIGHRNRNSKGHIISIED
PIEFIHQHQGCVITQREVGIDTESFEVALKNTLRQAPDVILIGEVRARETMDHAIAFAETGHLCLATLHANNANQALDRI
IHFFPADRHSQLWMDLSLNLRAIVAQQLIPTPDGQGRRACLEIFLNTPLAQDLIRKGEVHELKELMKKSNEQGMQTFDQA
LYELYDAGEITYEDALSHADSPNDLRLMIKLGSESDAEYLKHAADELSIQEDDSHRTRKF

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=961674 WKI13_RS20015 WP_018275011.1 4999678..5000820(-) (pilU) [Teredinibacter turnerae strain T7902]
ATGGATTTTGATCGCCTGCTGGCGTTGATGGTTGAAAAAGGTGCGTCGGATTTGTTTATTACGGCCGGTGTTCCGCCGTC
CATGAAATTACACGGTAAAGTGGTTCCCGTTTCGTCGACGCCCCTGTCGCCGGAAAAGAGCCGCGAAACTGTGCTCAGTG
TGATGAACGAAAAACAGCGCAAAGAGTTTTTGGAAAGCAAAGAGCTCAACTTTGCCATTAGCGCGCGAGGTGTTGGGCGG
TTTCGTGCCAGCGCGTTCTATCAGCGCAACCTGGCGGGCATGGTGTTGCGTAGAATCGAAACGCAAATTCCAAAAATCGA
TGACTTGGGCCTGCCGGAAACCATTAAAGAGCTGGCGATGGTAAAGCGCGGTTTGGTGCTGTTTGTGGGGGCGACCGGTA
CTGGTAAGTCCACCTCTCTCGCCGCGATGATCGGTCACCGCAATCGCAACTCCAAAGGGCATATTATTTCCATTGAAGAC
CCTATCGAATTTATTCATCAGCACCAGGGTTGTGTTATCACTCAGCGCGAAGTCGGTATTGATACGGAATCCTTTGAAGT
CGCGCTGAAAAATACCTTGCGACAAGCGCCAGACGTCATTCTGATTGGTGAGGTGCGTGCTCGTGAAACCATGGATCACG
CGATTGCGTTTGCGGAAACCGGACATCTCTGTCTCGCGACCTTGCACGCGAATAATGCGAACCAGGCGTTGGACCGTATT
ATTCACTTCTTTCCGGCCGATCGTCACAGCCAGCTGTGGATGGATTTGTCGTTAAACCTGCGAGCAATCGTTGCCCAGCA
GTTGATCCCTACGCCAGACGGGCAGGGGCGTCGCGCGTGCCTGGAAATATTCCTGAATACGCCGCTCGCTCAGGATTTAA
TTCGCAAGGGCGAAGTGCATGAGCTGAAAGAGCTGATGAAGAAATCCAACGAGCAGGGCATGCAAACGTTTGATCAGGCG
TTATACGAACTTTATGACGCGGGCGAAATTACGTATGAAGATGCGCTGTCGCATGCGGATTCGCCGAACGATTTGCGTCT
GATGATTAAGCTGGGTTCTGAGAGCGATGCCGAGTACCTGAAACACGCGGCCGATGAACTGAGTATTCAGGAAGACGATT
CTCATCGCACACGCAAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(50-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

77.632

100

0.776
  pilU Acinetobacter baylyi ADP1

66.667

93.947

0.626
  pilU Vibrio cholerae strain A1552

55.143

92.105

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.638

90.789

0.405
  pilT Legionella pneumophila strain Lp02

42.687

88.158

0.376
  pilT Legionella pneumophila strain ERS1305867

42.687

88.158

0.376
  pilT Pseudomonas aeruginosa PAK

40.87

90.789

0.371
  pilT Acinetobacter nosocomialis M2

40.643

90

0.366
  pilT Acinetobacter baumannii D1279779

40.643

90

0.366
  pilT Acinetobacter baumannii strain A118

40.643

90

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.246

88.684

0.366
  pilT Vibrio cholerae strain A1552

41.246

88.684

0.366
  pilT Pseudomonas stutzeri DSM 10701

40

90.789

0.363