Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   KAN5_RS11325 Genome accession   NZ_AP025589
Coordinates   2540783..2541214 (+) Length   143 a.a.
NCBI ID   WP_279612611.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. KAN5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2535783..2546214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAN5_RS11310 (KAN5_22100) ampE 2537271..2538122 (-) 852 WP_016708269.1 beta-lactamase regulator AmpE -
  KAN5_RS11315 (KAN5_22110) ampD 2538130..2538696 (-) 567 WP_138553799.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KAN5_RS11320 (KAN5_22120) nadC 2539390..2540235 (+) 846 WP_244389725.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KAN5_RS11325 (KAN5_22130) pilA/pilAI 2540783..2541214 (+) 432 WP_279612611.1 pilin Machinery gene
  KAN5_RS11330 - 2541252..2542523 (+) 1272 WP_244389726.1 O-antigen ligase family protein -
  KAN5_RS11335 (KAN5_22160) pilC 2542537..2543766 (+) 1230 WP_244389727.1 type II secretion system F family protein Machinery gene
  KAN5_RS11340 (KAN5_22170) pilD 2544268..2545167 (+) 900 WP_279612612.1 A24 family peptidase Machinery gene
  KAN5_RS11345 (KAN5_22180) coaE 2545169..2545798 (+) 630 WP_244389728.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14832.92 Da        Isoelectric Point: 7.5885

>NTDB_id=92880 KAN5_RS11325 WP_279612611.1 2540783..2541214(+) (pilA/pilAI) [Pseudoalteromonas sp. KAN5]
MEKMTQQSQKGFTLIELMIVVAIIGILAAIALPQYQDYTAKSQAGSAFAEVSALKTQFEVAVNEGKTPSLTNTEAGFIGQ
TANGGTYCDIALTGTTAIACTMKNGNAGKINGKTLTLNRSADGVWSCATGNGLEAKFKPGNCS

Nucleotide


Download         Length: 432 bp        

>NTDB_id=92880 KAN5_RS11325 WP_279612611.1 2540783..2541214(+) (pilA/pilAI) [Pseudoalteromonas sp. KAN5]
ATGGAAAAAATGACACAACAAAGTCAAAAGGGTTTTACCCTAATTGAATTAATGATTGTAGTAGCAATCATCGGTATTTT
GGCAGCAATTGCGTTGCCACAATATCAAGATTATACAGCAAAATCACAAGCTGGCTCAGCCTTCGCTGAAGTATCAGCAC
TCAAAACCCAATTTGAAGTTGCTGTTAACGAAGGAAAAACACCATCTCTTACAAATACAGAGGCTGGTTTTATTGGTCAA
ACAGCTAATGGTGGAACATACTGTGATATCGCACTAACTGGGACAACCGCTATTGCGTGTACAATGAAAAACGGCAACGC
TGGAAAAATAAATGGTAAAACACTGACTCTAAACCGCAGCGCAGATGGAGTTTGGTCTTGCGCAACTGGTAATGGCTTAG
AAGCCAAGTTCAAGCCAGGTAATTGCAGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

63.38

99.301

0.629
  pilA/pilAII Pseudomonas stutzeri DSM 10701

52.113

99.301

0.517
  pilA Acinetobacter baumannii strain A118

51.429

97.902

0.504
  pilA Pseudomonas aeruginosa PAK

47.333

100

0.497
  pilA/pilA1 Eikenella corrodens VA1

41.29

100

0.448
  pilA Vibrio cholerae C6706

42.282

100

0.441
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.282

100

0.441
  pilA Vibrio cholerae strain A1552

42.282

100

0.441
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

32.787

100

0.42
  pilE Neisseria gonorrhoeae strain FA1090

37.013

100

0.399
  pilA2 Legionella pneumophila strain ERS1305867

41.176

95.105

0.392
  pilA2 Legionella pneumophila str. Paris

41.176

95.105

0.392
  pilA Acinetobacter nosocomialis M2

40.146

95.804

0.385
  pilA Vibrio parahaemolyticus RIMD 2210633

42.188

89.51

0.378
  comP Acinetobacter baylyi ADP1

36.552

100

0.371
  pilE Neisseria gonorrhoeae MS11

42.4

87.413

0.371

Multiple sequence alignment