Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU43_RS21240 Genome accession   NZ_AP025284
Coordinates   4586521..4587555 (-) Length   344 a.a.
NCBI ID   WP_091360370.1    Uniprot ID   -
Organism   Amphritea atlantica strain JCM 14776     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4581521..4592555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU43_RS21215 ruvX 4581957..4582433 (+) 477 WP_091360379.1 Holliday junction resolvase RuvX -
  OCU43_RS21220 pyrR 4582414..4582929 (+) 516 WP_091360378.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  OCU43_RS21225 - 4582949..4583950 (+) 1002 WP_091360376.1 aspartate carbamoyltransferase catalytic subunit -
  OCU43_RS21230 - 4583961..4585235 (+) 1275 WP_091360374.1 dihydroorotase -
  OCU43_RS21235 pilU 4585340..4586506 (-) 1167 WP_091360372.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU43_RS21240 pilT 4586521..4587555 (-) 1035 WP_091360370.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU43_RS21245 - 4587892..4588602 (+) 711 WP_091360369.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU43_RS21250 proC 4588645..4589469 (+) 825 WP_091360367.1 pyrroline-5-carboxylate reductase -
  OCU43_RS21255 - 4589615..4590172 (+) 558 WP_091360365.1 YggT family protein -
  OCU43_RS21260 - 4590181..4590474 (+) 294 WP_091360362.1 DUF167 family protein -
  OCU43_RS21265 - 4590616..4591782 (+) 1167 WP_091360361.1 homoserine O-acetyltransferase -
  OCU43_RS21270 metW 4591782..4592372 (+) 591 WP_091360359.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38216.91 Da        Isoelectric Point: 7.2380

>NTDB_id=91051 OCU43_RS21240 WP_091360370.1 4586521..4587555(-) (pilT) [Amphritea atlantica strain JCM 14776]
MDITELLSFTVQQGASDLHITAGMPPVIRVDGEVRRIKLPSLEHKQVHTLIYDIMNDKQRKDYEERFETDFSFEVPGLAR
FRVNAYNQNRGAAAVFRTIPSKVLTMDDLGMGQVFRNLSNNARGLVLVTGPTGSGKSTTLAAMVDYINETRNEHILTIED
PIEFVHQSKKCLVNQREVHRDTLGFEEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKSMVRSMLSESLQGVISQTLLKKPKGGRVAAHEIMVGTPAIRNLIREDKVAQMYSAIQTGAAFGMVTLDQSL
TDLMQKGLITRESARAKALNPKNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=91051 OCU43_RS21240 WP_091360370.1 4586521..4587555(-) (pilT) [Amphritea atlantica strain JCM 14776]
ATGGATATTACAGAACTCTTATCGTTTACCGTTCAGCAGGGAGCGTCCGACCTCCATATAACCGCCGGTATGCCACCGGT
TATCCGGGTTGATGGCGAGGTCCGCAGAATTAAGCTGCCCTCTCTGGAGCATAAGCAGGTTCACACGCTGATTTACGATA
TCATGAATGATAAGCAGCGTAAGGACTACGAGGAGCGGTTTGAAACTGACTTCTCCTTCGAGGTGCCCGGTCTGGCCCGT
TTCCGTGTTAACGCCTATAACCAGAACCGTGGTGCGGCAGCGGTATTCCGGACCATCCCCAGCAAAGTTCTGACGATGGA
TGATCTGGGCATGGGACAGGTGTTCCGCAATCTTTCCAATAATGCCCGTGGCCTGGTGCTGGTAACCGGACCGACCGGCT
CGGGTAAGAGTACCACCCTGGCGGCGATGGTGGATTACATCAATGAGACCCGCAACGAGCACATCCTCACGATTGAGGAC
CCGATAGAATTTGTCCATCAGAGCAAGAAGTGTCTGGTTAACCAGCGGGAGGTGCACCGGGATACGCTCGGGTTTGAGGA
GGCGTTGCGTTCCGCCCTGCGTGAGGACCCGGATATTATTCTGGTGGGTGAGATGCGGGATCTGGAAACTATCCGTCTGG
CGCTGACCGCTGCAGAAACCGGTCACCTGGTGTTTGGTACACTGCACACCACCTCAGCGGCAAAAACGATTGACCGTATC
ATCGACGTGTTCCCGGCAGCAGAAAAATCGATGGTGCGTTCGATGCTGTCAGAATCTCTCCAGGGGGTTATCTCCCAGAC
TCTGCTGAAGAAGCCGAAAGGCGGACGGGTGGCGGCCCATGAGATTATGGTGGGTACGCCAGCAATTCGTAACCTGATAC
GTGAAGATAAGGTAGCGCAGATGTACTCTGCCATTCAGACCGGTGCGGCATTTGGGATGGTGACTCTGGATCAGTCGCTC
ACCGACCTGATGCAGAAAGGTTTGATTACCCGTGAATCAGCCCGTGCTAAGGCACTGAATCCTAAGAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

82.558

100

0.826
  pilT Pseudomonas stutzeri DSM 10701

81.977

100

0.82
  pilT Acinetobacter baumannii D1279779

80.523

100

0.805
  pilT Acinetobacter baumannii strain A118

80.523

100

0.805
  pilT Acinetobacter nosocomialis M2

80.523

100

0.805
  pilT Acinetobacter baylyi ADP1

79.07

100

0.791
  pilT Legionella pneumophila strain Lp02

74.709

100

0.747
  pilT Legionella pneumophila strain ERS1305867

74.709

100

0.747
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.766

97.965

0.654
  pilT Vibrio cholerae strain A1552

66.766

97.965

0.654
  pilT Neisseria meningitidis 8013

64.348

100

0.645
  pilT Neisseria gonorrhoeae MS11

64.058

100

0.642
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.509
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39
  pilB Acinetobacter baylyi ADP1

31.566

100

0.363

Multiple sequence alignment