Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU43_RS21235 Genome accession   NZ_AP025284
Coordinates   4585340..4586506 (-) Length   388 a.a.
NCBI ID   WP_091360372.1    Uniprot ID   -
Organism   Amphritea atlantica strain JCM 14776     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4580340..4591506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU43_RS21210 - 4581338..4581919 (+) 582 WP_091360381.1 YqgE/AlgH family protein -
  OCU43_RS21215 ruvX 4581957..4582433 (+) 477 WP_091360379.1 Holliday junction resolvase RuvX -
  OCU43_RS21220 pyrR 4582414..4582929 (+) 516 WP_091360378.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  OCU43_RS21225 - 4582949..4583950 (+) 1002 WP_091360376.1 aspartate carbamoyltransferase catalytic subunit -
  OCU43_RS21230 - 4583961..4585235 (+) 1275 WP_091360374.1 dihydroorotase -
  OCU43_RS21235 pilU 4585340..4586506 (-) 1167 WP_091360372.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU43_RS21240 pilT 4586521..4587555 (-) 1035 WP_091360370.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU43_RS21245 - 4587892..4588602 (+) 711 WP_091360369.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU43_RS21250 proC 4588645..4589469 (+) 825 WP_091360367.1 pyrroline-5-carboxylate reductase -
  OCU43_RS21255 - 4589615..4590172 (+) 558 WP_091360365.1 YggT family protein -
  OCU43_RS21260 - 4590181..4590474 (+) 294 WP_091360362.1 DUF167 family protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43091.16 Da        Isoelectric Point: 5.2838

>NTDB_id=91050 OCU43_RS21235 WP_091360372.1 4585340..4586506(-) (pilU) [Amphritea atlantica strain JCM 14776]
MDITQLLKVMTDRQASDLFITAGARPSIKVDGTIKPLTKEALKPAQARSLTYSTMNDKQLAEFEGTRECNFAISAPGIGR
FRVSAFFQRNSPGMVLRRINTYIPTMEELNLPPVLKDLAMTKRGLILFVGGTSTGKSTSLASMIDYRNANTAGHIITIED
PIEYIHEHKQSVITQREVGLDTDSFEVALKNTLRQAPDVILMGEIRSADTMGYGLTFAETGHLCMATLHANNANQALDRI
ISFFPPEHHAQIWMDLSLNLKAIVAQQLLPTKDGKGRRAVVEVLINTPLMQDLIRKGEVHELKEVIKKSTNLGMQTFDQA
LYNEYKEGTITYDVAIAHADSPNDLRLMIKLNSETNPDINDASDDSGFFLQDDDDFLQNEGSLDVKGM

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=91050 OCU43_RS21235 WP_091360372.1 4585340..4586506(-) (pilU) [Amphritea atlantica strain JCM 14776]
TTGGATATTACCCAGCTACTTAAGGTTATGACTGACCGACAAGCATCCGATCTGTTTATCACCGCCGGAGCCCGTCCCAG
CATCAAGGTTGATGGTACGATTAAGCCATTAACCAAAGAAGCTCTGAAACCCGCTCAGGCACGTTCGCTGACCTATAGCA
CCATGAACGATAAGCAACTGGCCGAGTTTGAGGGCACCCGCGAGTGTAACTTTGCTATCAGTGCTCCGGGGATCGGTCGT
TTCCGGGTGAGTGCATTTTTTCAGCGTAACTCACCGGGTATGGTACTGCGGCGCATCAACACCTATATCCCAACGATGGA
GGAGCTGAACCTGCCGCCGGTGCTCAAGGATCTGGCGATGACCAAGCGGGGGCTTATCCTGTTTGTCGGCGGTACCAGTA
CCGGTAAATCAACGTCACTGGCGTCGATGATCGATTACCGCAATGCCAATACTGCCGGGCATATCATTACCATTGAGGAT
CCGATCGAATATATCCATGAGCATAAGCAGAGTGTGATCACCCAGCGGGAAGTGGGTCTGGATACCGATTCCTTTGAGGT
GGCTCTGAAAAATACCCTGCGTCAGGCCCCGGATGTGATTCTGATGGGGGAGATTCGCAGTGCCGATACGATGGGCTATG
GTCTGACCTTTGCTGAGACTGGCCACCTCTGTATGGCAACACTGCACGCCAACAACGCCAACCAGGCTCTGGACCGGATC
ATCAGTTTCTTCCCCCCTGAACACCATGCCCAGATCTGGATGGATCTGTCGCTTAACCTGAAAGCAATTGTGGCGCAGCA
ACTGCTGCCGACAAAGGATGGTAAAGGTCGCCGGGCAGTGGTGGAAGTGCTGATTAATACCCCTCTGATGCAGGACCTGA
TCCGTAAAGGCGAAGTGCATGAGCTGAAAGAGGTAATCAAAAAGTCCACTAACCTGGGGATGCAGACCTTCGATCAGGCG
CTTTACAATGAGTATAAAGAGGGGACTATCACCTATGATGTGGCGATCGCCCATGCGGATTCACCGAACGACCTGCGTCT
GATGATTAAACTTAATTCGGAAACCAATCCGGATATCAATGATGCATCGGACGACTCCGGCTTCTTCCTGCAGGATGACG
ATGATTTTCTGCAAAATGAAGGGTCGCTGGACGTTAAAGGGATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.2

96.649

0.649
  pilU Acinetobacter baylyi ADP1

61.602

93.299

0.575
  pilU Vibrio cholerae strain A1552

58.857

90.206

0.531
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.348

88.918

0.394
  pilT Pseudomonas aeruginosa PAK

42.319

88.918

0.376
  pilT Acinetobacter baylyi ADP1

42.398

88.144

0.374
  pilT Pseudomonas stutzeri DSM 10701

42.029

88.918

0.374
  pilT Acinetobacter nosocomialis M2

42.105

88.144

0.371
  pilT Acinetobacter baumannii D1279779

42.105

88.144

0.371
  pilT Acinetobacter baumannii strain A118

42.105

88.144

0.371

Multiple sequence alignment