Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU87_RS02380 Genome accession   NZ_AP024850
Coordinates   481387..482424 (+) Length   345 a.a.
NCBI ID   WP_062689549.1    Uniprot ID   A0AAW7Y766
Organism   Photobacterium sanguinicancri strain CECT 7579     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 476387..487424
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU87_RS02350 - 477504..478097 (-) 594 WP_261857763.1 XTP/dITP diphosphatase -
  OCU87_RS02355 - 478255..478683 (-) 429 WP_094957444.1 DUF4426 domain-containing protein -
  OCU87_RS02360 yggU 478870..479166 (-) 297 WP_261857764.1 DUF167 family protein YggU -
  OCU87_RS02365 - 479163..479720 (-) 558 WP_094957446.1 YggT family protein -
  OCU87_RS02370 proC 479730..480548 (-) 819 WP_261857765.1 pyrroline-5-carboxylate reductase -
  OCU87_RS02375 - 480653..481363 (-) 711 WP_062689547.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU87_RS02380 pilT 481387..482424 (+) 1038 WP_062689549.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU87_RS02385 pilU 482434..483540 (+) 1107 WP_094957449.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU87_RS02390 ruvX 483914..484345 (-) 432 WP_062689551.1 Holliday junction resolvase RuvX -
  OCU87_RS02395 - 484342..484905 (-) 564 WP_094957453.1 YqgE/AlgH family protein -
  OCU87_RS02400 gshB 485072..486025 (-) 954 WP_261857766.1 glutathione synthase -
  OCU87_RS02405 rsmE 486042..486773 (-) 732 WP_062689553.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38204.60 Da        Isoelectric Point: 6.2698

>NTDB_id=88762 OCU87_RS02380 WP_062689549.1 481387..482424(+) (pilT) [Photobacterium sanguinicancri strain CECT 7579]
MDITELLDFSVKHNASDLHLSAGVPPIIRVDGDVRKLSLPALAHSEVHRLVFDIMNDAQRREYEEKLEVDFSFELPDVGR
FRVNAFHQSRGSAAVFRTIPMHVPTLESLSVPDIFYDIAQLPRGLVLVTGATGSGKSTTIAALVDYINENYNRHILTIED
PVEFVHHSKRSLINQREVHRDTHSFQAALRSSLREDPDVILVGELRDQETISLALTAAETGHLVLGTLHTSSAAKTIDRI
IDVFPGNDKSMVRSMLSESLRAVISQSLLKCTSGGRVASHEIMMATPAIRNLIREDKVAQMYSMIQTGSAVGMQTMEQSI
KMLVAQGTVEAEEGRRILDSAANRM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=88762 OCU87_RS02380 WP_062689549.1 481387..482424(+) (pilT) [Photobacterium sanguinicancri strain CECT 7579]
ATGGATATCACCGAACTACTGGACTTTAGTGTAAAGCATAATGCCTCTGATCTGCATCTTTCTGCAGGGGTTCCACCAAT
TATTCGTGTTGATGGTGATGTACGTAAACTGAGTTTACCTGCTTTAGCGCATAGCGAAGTGCATCGCTTAGTTTTTGATA
TTATGAATGATGCTCAGCGTCGAGAGTATGAAGAAAAGCTCGAAGTGGACTTTTCGTTCGAATTACCTGATGTGGGTCGC
TTTAGGGTGAATGCGTTTCACCAGTCTCGTGGTAGTGCTGCTGTTTTTCGTACCATACCTATGCATGTGCCAACGTTGGA
ATCACTCAGTGTTCCTGACATTTTCTATGATATTGCCCAGCTACCCCGTGGATTAGTGTTAGTGACGGGGGCGACAGGAT
CGGGTAAATCAACCACGATTGCAGCTTTAGTGGATTACATTAACGAAAACTACAACCGTCATATTCTAACGATTGAAGAC
CCGGTGGAATTTGTTCATCACAGCAAACGTAGTTTGATTAATCAGCGTGAAGTGCACCGTGATACCCACAGTTTTCAAGC
AGCACTACGTTCATCGCTGCGTGAAGATCCCGATGTTATCTTAGTGGGGGAGTTACGCGATCAAGAAACAATCAGCTTGG
CACTAACAGCGGCAGAAACGGGTCACCTTGTATTAGGAACCTTGCATACCAGTTCGGCGGCTAAAACTATCGACAGGATC
ATCGATGTTTTCCCTGGTAATGATAAATCCATGGTGCGCTCGATGTTATCGGAATCATTGCGGGCGGTAATTTCACAAAG
CCTGCTGAAATGTACCAGTGGTGGGCGTGTTGCATCACATGAAATTATGATGGCGACCCCAGCCATTCGAAATTTAATTC
GTGAAGATAAAGTGGCGCAAATGTATTCGATGATTCAAACCGGCTCGGCAGTCGGTATGCAGACCATGGAGCAGAGTATC
AAAATGCTAGTGGCGCAAGGAACGGTCGAAGCGGAAGAAGGCCGACGTATCCTCGACAGTGCCGCAAATCGAATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

78.979

96.522

0.762
  pilT Vibrio cholerae strain A1552

78.979

96.522

0.762
  pilT Acinetobacter baumannii D1279779

72.537

97.101

0.704
  pilT Acinetobacter baumannii strain A118

72.537

97.101

0.704
  pilT Acinetobacter nosocomialis M2

72.239

97.101

0.701
  pilT Acinetobacter baylyi ADP1

70

98.551

0.69
  pilT Pseudomonas aeruginosa PAK

68.955

97.101

0.67
  pilT Pseudomonas stutzeri DSM 10701

68.955

97.101

0.67
  pilT Legionella pneumophila strain Lp02

68.693

95.362

0.655
  pilT Legionella pneumophila strain ERS1305867

68.693

95.362

0.655
  pilT Neisseria meningitidis 8013

66.469

97.681

0.649
  pilT Neisseria gonorrhoeae MS11

66.172

97.681

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.746

97.101

0.493
  pilU Vibrio cholerae strain A1552

41.228

99.13

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.812

0.394
  pilU Acinetobacter baylyi ADP1

37.101

100

0.371

Multiple sequence alignment