Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU87_RS02385 Genome accession   NZ_AP024850
Coordinates   482434..483540 (+) Length   368 a.a.
NCBI ID   WP_094957449.1    Uniprot ID   A0AAW7Y6E1
Organism   Photobacterium sanguinicancri strain CECT 7579     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 477434..488540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU87_RS02350 - 477504..478097 (-) 594 WP_261857763.1 XTP/dITP diphosphatase -
  OCU87_RS02355 - 478255..478683 (-) 429 WP_094957444.1 DUF4426 domain-containing protein -
  OCU87_RS02360 yggU 478870..479166 (-) 297 WP_261857764.1 DUF167 family protein YggU -
  OCU87_RS02365 - 479163..479720 (-) 558 WP_094957446.1 YggT family protein -
  OCU87_RS02370 proC 479730..480548 (-) 819 WP_261857765.1 pyrroline-5-carboxylate reductase -
  OCU87_RS02375 - 480653..481363 (-) 711 WP_062689547.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU87_RS02380 pilT 481387..482424 (+) 1038 WP_062689549.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU87_RS02385 pilU 482434..483540 (+) 1107 WP_094957449.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU87_RS02390 ruvX 483914..484345 (-) 432 WP_062689551.1 Holliday junction resolvase RuvX -
  OCU87_RS02395 - 484342..484905 (-) 564 WP_094957453.1 YqgE/AlgH family protein -
  OCU87_RS02400 gshB 485072..486025 (-) 954 WP_261857766.1 glutathione synthase -
  OCU87_RS02405 rsmE 486042..486773 (-) 732 WP_062689553.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU87_RS02410 - 486920..487633 (-) 714 WP_261857767.1 endonuclease -
  OCU87_RS02415 - 487782..488264 (-) 483 WP_261857768.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41196.19 Da        Isoelectric Point: 5.5977

>NTDB_id=88763 OCU87_RS02385 WP_094957449.1 482434..483540(+) (pilU) [Photobacterium sanguinicancri strain CECT 7579]
MILQDILNQMVASKASDLYITVDSPCLLKIDGQLQAVGERLDRAGVDAFFAEALDPKLTHEFETTREANFALVRDELRFR
VSAFWQRELPGMVVRRIETQIPKLDELSLPIDMERMALAKRGLVLVVGATGSGKSTSMAALTGFRNRQRSGHILTVEDPI
EFVHQHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTPETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERRDQFLFDLSLNLKCVLAQQLIPDAHGVGRHAAFELLINTPRVADLIRRGELHEIKDTMAKGTDSGMMTFDQSLF
ELFNGGKITEQDALHHADSANDLRLMIKKGDKFQQGNSSLEGVTVSFD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=88763 OCU87_RS02385 WP_094957449.1 482434..483540(+) (pilU) [Photobacterium sanguinicancri strain CECT 7579]
ATGATTTTACAAGACATTCTTAATCAGATGGTGGCGAGTAAAGCCTCGGATCTTTACATCACGGTTGATTCACCGTGTTT
GTTAAAGATTGATGGTCAGTTGCAAGCTGTGGGTGAAAGGCTGGATCGTGCGGGTGTTGATGCTTTTTTTGCTGAAGCGC
TGGATCCTAAATTAACCCACGAATTTGAGACCACCCGTGAAGCCAATTTTGCGTTAGTGCGTGATGAACTTCGGTTTCGT
GTTAGTGCTTTTTGGCAGCGTGAGTTGCCGGGCATGGTGGTGCGACGTATTGAAACCCAGATCCCTAAATTGGATGAGTT
ATCACTGCCGATTGATATGGAGCGGATGGCACTGGCAAAACGTGGTTTGGTCCTTGTTGTTGGTGCAACGGGATCGGGTA
AATCGACGTCGATGGCGGCTTTGACTGGATTTCGCAATCGACAGCGTAGTGGTCATATCTTAACGGTAGAAGATCCGATT
GAGTTTGTTCACCAACACGACAAGTGCATAGTGACCCAACGTGAAGTCGGACTGGATACCGAAAGCTACGAAGTTGCATT
GAAAAACTCACTGCGCCAAGCGCCCGATATGATTTTGATTGGTGAAATTCGTACGCCTGAAACTATGGAATACGCGATGA
ACTTTGCCGAAACGGGCCACTTATGTATGGCAACCCTACACGCCAATAATGCCAACCAAGCGCTTGAGCGTATTTTGCAT
TTGGTGCCGAAAGAGCGCCGTGATCAGTTTTTGTTTGATCTGTCACTGAACCTTAAATGTGTATTGGCACAGCAGTTGAT
CCCCGATGCGCATGGGGTAGGTCGACACGCGGCCTTTGAGTTGTTGATCAATACACCGCGAGTTGCCGATCTGATCCGAC
GCGGAGAATTACACGAGATCAAAGACACGATGGCGAAAGGGACTGACTCGGGAATGATGACGTTTGATCAGTCACTGTTC
GAGTTGTTTAATGGTGGAAAGATCACCGAGCAAGATGCTCTACACCATGCGGATTCTGCCAATGATTTACGCTTAATGAT
CAAAAAAGGCGATAAGTTTCAGCAAGGCAATAGCAGCTTAGAAGGGGTGACCGTTAGCTTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.649

100

0.69
  pilU Pseudomonas stutzeri DSM 10701

56.983

97.283

0.554
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.84

91.576

0.383
  pilT Legionella pneumophila strain Lp02

40.299

91.033

0.367
  pilT Legionella pneumophila strain ERS1305867

40.299

91.033

0.367

Multiple sequence alignment