Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q9312_RS11745 Genome accession   NZ_CP133548
Coordinates   2614731..2615765 (-) Length   344 a.a.
NCBI ID   WP_309201043.1    Uniprot ID   A0AA51RQR1
Organism   Pleionea litopenaei strain HL-JVS1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2609731..2620765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9312_RS11715 (Q9312_11715) pilG 2609782..2610180 (-) 399 WP_309201037.1 twitching motility response regulator PilG Regulator
  Q9312_RS11720 (Q9312_11720) gshB 2610497..2611450 (+) 954 WP_309201038.1 glutathione synthase -
  Q9312_RS11725 (Q9312_11725) - 2611562..2612455 (+) 894 WP_309201039.1 TonB family protein -
  Q9312_RS11730 (Q9312_11730) - 2612509..2613069 (+) 561 WP_309201040.1 YqgE/AlgH family protein -
  Q9312_RS11735 (Q9312_11735) ruvX 2613062..2613493 (+) 432 WP_309201041.1 Holliday junction resolvase RuvX -
  Q9312_RS11740 (Q9312_11740) pilU 2613563..2614705 (-) 1143 WP_309201042.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q9312_RS11745 (Q9312_11745) pilT 2614731..2615765 (-) 1035 WP_309201043.1 type IV pilus twitching motility protein PilT Machinery gene
  Q9312_RS11750 (Q9312_11750) - 2615965..2616675 (+) 711 WP_309201044.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q9312_RS11755 (Q9312_11755) proC 2616676..2617497 (+) 822 WP_309201045.1 pyrroline-5-carboxylate reductase -
  Q9312_RS11760 (Q9312_11760) - 2617653..2618234 (+) 582 WP_309201046.1 YggT family protein -
  Q9312_RS11765 (Q9312_11765) - 2618418..2620385 (+) 1968 WP_309201047.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38169.87 Da        Isoelectric Point: 6.3710

>NTDB_id=874809 Q9312_RS11745 WP_309201043.1 2614731..2615765(-) (pilT) [Pleionea litopenaei strain HL-JVS1]
MDITELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINVPPLDHKTVHSLVYDIMNDKQRKDFEEFLETDFSFELPGIAR
FRVNAFNHNRGAGAVFRTIPSQVLTLEELGAPAVFKEICMHPRGLVLVTGPTGSGKSTTLAGMIDYINDNDYGHILTVED
PIEFVHESKRCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLMAVISQTLLKKVGGGRVAAHEIMVGTPAIRNLIREDKIAQMYSSIQTGAQHGMQTLDQCL
KDLLAKGLITRQDAREKAKTKEDF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=874809 Q9312_RS11745 WP_309201043.1 2614731..2615765(-) (pilT) [Pleionea litopenaei strain HL-JVS1]
ATGGATATTACTGAGTTACTTGCTTTTAGTGTTAAGAACAAAGCATCGGATTTGCACTTATCGGCAGGCCTGCCGCCGAT
GATTCGTGTCGATGGCGATGTGCGCCGAATTAATGTGCCGCCTCTTGATCATAAAACGGTACATAGCCTTGTTTATGACA
TCATGAACGATAAGCAGCGTAAAGACTTTGAAGAGTTTTTAGAAACTGACTTCTCATTCGAATTACCAGGTATCGCGCGT
TTTCGTGTTAATGCATTTAACCACAATCGCGGAGCGGGTGCGGTATTTCGTACCATCCCATCGCAAGTTTTAACCCTCGA
AGAGTTAGGGGCGCCGGCTGTTTTTAAAGAAATCTGTATGCACCCTCGTGGCTTAGTACTCGTCACAGGACCTACTGGTT
CAGGTAAAAGTACCACGCTTGCAGGAATGATTGATTACATCAATGACAACGATTACGGACATATTTTGACGGTCGAGGAC
CCCATCGAATTTGTGCACGAAAGTAAGCGCTGTTTGATTAACCAGCGTGAAGTTCATCGTGATACGCTAGGCTTTAATGA
AGCATTGCGCTCAGCGCTACGGGAAGATCCTGACATCATCCTAGTTGGTGAGATGCGTGACCTTGAAACGATTCGTTTGG
CATTGACTGCCGCAGAAACGGGGCATTTGGTGTTTGGTACTCTGCATACGTCATCCGCCGCCAAAACCATTGACCGTATT
GTCGACGTTTTCCCTGCTGCTGAAAAAGACATGGTTCGTTCGATGTTATCTGAATCATTGATGGCGGTAATTTCTCAGAC
ATTATTGAAAAAAGTCGGCGGTGGCCGTGTCGCGGCGCACGAAATAATGGTCGGTACGCCAGCGATTCGTAACTTGATTC
GTGAAGATAAAATCGCGCAGATGTACAGCTCGATTCAAACGGGTGCGCAACACGGGATGCAAACACTTGATCAGTGTCTA
AAAGACCTCCTCGCGAAGGGCTTGATTACGCGACAAGATGCTCGTGAGAAAGCCAAGACCAAAGAAGATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

84.302

100

0.843
  pilT Pseudomonas aeruginosa PAK

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

82.849

100

0.828
  pilT Acinetobacter baumannii strain A118

82.267

100

0.823
  pilT Acinetobacter baumannii D1279779

82.267

100

0.823
  pilT Acinetobacter nosocomialis M2

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

74.709

100

0.747
  pilT Legionella pneumophila strain ERS1305867

74.709

100

0.747
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

97.965

0.689
  pilT Vibrio cholerae strain A1552

70.326

97.965

0.689
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Vibrio cholerae strain A1552

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401