Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q9312_RS11740 Genome accession   NZ_CP133548
Coordinates   2613563..2614705 (-) Length   380 a.a.
NCBI ID   WP_309201042.1    Uniprot ID   A0AA51RQS9
Organism   Pleionea litopenaei strain HL-JVS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2608563..2619705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9312_RS11705 (Q9312_11705) - 2608778..2609320 (-) 543 WP_309201036.1 chemotaxis protein CheW -
  Q9312_RS11710 (Q9312_11710) pilH 2609367..2609732 (-) 366 WP_144392326.1 twitching motility response regulator PilH -
  Q9312_RS11715 (Q9312_11715) pilG 2609782..2610180 (-) 399 WP_309201037.1 twitching motility response regulator PilG Regulator
  Q9312_RS11720 (Q9312_11720) gshB 2610497..2611450 (+) 954 WP_309201038.1 glutathione synthase -
  Q9312_RS11725 (Q9312_11725) - 2611562..2612455 (+) 894 WP_309201039.1 TonB family protein -
  Q9312_RS11730 (Q9312_11730) - 2612509..2613069 (+) 561 WP_309201040.1 YqgE/AlgH family protein -
  Q9312_RS11735 (Q9312_11735) ruvX 2613062..2613493 (+) 432 WP_309201041.1 Holliday junction resolvase RuvX -
  Q9312_RS11740 (Q9312_11740) pilU 2613563..2614705 (-) 1143 WP_309201042.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q9312_RS11745 (Q9312_11745) pilT 2614731..2615765 (-) 1035 WP_309201043.1 type IV pilus twitching motility protein PilT Machinery gene
  Q9312_RS11750 (Q9312_11750) - 2615965..2616675 (+) 711 WP_309201044.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q9312_RS11755 (Q9312_11755) proC 2616676..2617497 (+) 822 WP_309201045.1 pyrroline-5-carboxylate reductase -
  Q9312_RS11760 (Q9312_11760) - 2617653..2618234 (+) 582 WP_309201046.1 YggT family protein -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42258.64 Da        Isoelectric Point: 6.5044

>NTDB_id=874808 Q9312_RS11740 WP_309201042.1 2613563..2614705(-) (pilU) [Pleionea litopenaei strain HL-JVS1]
MDFEALLKLMVQKKASDLFITAGIPPSIKINGKIMPVTQRALNGEKAREVVLSVMNEEQRREFARGHECNFAISASGIGR
FRVSAFQQRNEVGMVLRRIETTIPTLDELRLPTVLKKLSLTKRGLIIFVGATGTGKSTSLAAMIGYRNMTTTGHIITIED
PIEFVHQHNKCIITQREVGIDTDSFETALKNTLRQAPDVILIGEVRTSETMDYAVAFAETGHLCMCTLHANNANQALDRI
IHFFPEEKRNQLLLDLSLNLRAVIAQQLIPTPDGKGRRAAIEVLINTPLVADHIRKGEMHLIKDLMAKSTEHGMQTFDQA
LFRLFKSGEVTYEDALHHADSPNDLRLMIKLDSSESDPELQIGSGAAASMSLEETDENPF

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=874808 Q9312_RS11740 WP_309201042.1 2613563..2614705(-) (pilU) [Pleionea litopenaei strain HL-JVS1]
ATGGATTTTGAAGCTCTTTTAAAATTGATGGTGCAAAAGAAAGCCTCGGATTTATTTATTACCGCTGGCATTCCACCTTC
AATCAAAATAAATGGAAAAATCATGCCGGTTACACAACGGGCATTGAATGGCGAGAAAGCTCGAGAAGTTGTTCTTTCGG
TTATGAATGAGGAACAGCGACGAGAATTTGCTCGCGGTCACGAGTGTAACTTTGCGATTAGCGCATCCGGTATTGGTCGA
TTCCGGGTTAGCGCGTTCCAGCAACGAAATGAAGTGGGTATGGTCTTGCGTCGAATTGAAACGACTATCCCGACGCTAGA
TGAATTGCGTTTGCCAACCGTGTTAAAGAAACTCTCACTCACCAAGCGTGGACTGATTATTTTTGTCGGTGCGACCGGTA
CGGGTAAATCAACCTCTTTGGCGGCCATGATTGGATATCGCAATATGACCACCACTGGTCACATCATTACCATTGAAGAT
CCGATTGAATTTGTTCATCAACATAACAAATGCATTATTACCCAGCGTGAAGTGGGTATTGATACTGACTCCTTTGAAAC
CGCGTTGAAAAATACGTTACGACAAGCGCCTGACGTTATTCTGATTGGTGAGGTACGAACGTCTGAAACCATGGATTACG
CAGTGGCTTTTGCCGAAACTGGGCATCTGTGTATGTGTACTTTGCATGCGAACAACGCAAACCAGGCACTCGATCGAATC
ATTCACTTCTTTCCTGAAGAAAAGCGAAATCAATTGCTGTTGGATTTGTCGTTAAATTTACGTGCGGTTATTGCTCAGCA
GTTAATTCCGACACCCGACGGTAAAGGCCGTCGCGCCGCGATTGAAGTATTGATTAATACACCTTTGGTGGCCGATCATA
TTCGTAAAGGTGAAATGCATTTGATCAAAGACTTAATGGCTAAGTCCACTGAACACGGTATGCAAACCTTTGACCAAGCA
CTGTTTCGACTATTTAAAAGCGGTGAAGTTACTTACGAAGATGCGCTTCATCATGCCGACTCGCCAAATGATTTACGCTT
GATGATCAAACTAGACAGTTCAGAGAGCGATCCTGAATTGCAGATTGGTTCTGGTGCTGCGGCAAGCATGAGTTTAGAAG
AAACCGATGAAAACCCCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

72.559

99.737

0.724
  pilU Acinetobacter baylyi ADP1

65.738

94.474

0.621
  pilU Vibrio cholerae strain A1552

54.96

98.158

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.638

90.789

0.405
  pilT Acinetobacter baylyi ADP1

40.936

90

0.368
  pilT Neisseria meningitidis 8013

41.056

89.737

0.368
  pilT Neisseria gonorrhoeae MS11

41.056

89.737

0.368
  pilT Vibrio cholerae strain A1552

41.916

87.895

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.916

87.895

0.368
  pilT Pseudomonas aeruginosa PAK

40

90.789

0.363
  pilT Acinetobacter baumannii strain A118

40.058

90

0.361
  pilT Acinetobacter baumannii D1279779

40.058

90

0.361
  pilT Acinetobacter nosocomialis M2

40.058

90

0.361