Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Atep_RS06945 Genome accession   NZ_AP024563
Coordinates   1438758..1439813 (+) Length   351 a.a.
NCBI ID   WP_213381083.1    Uniprot ID   -
Organism   Allochromatium tepidum strain NZ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1433758..1444813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Atep_RS06920 (Atep_13480) glnK 1433948..1434286 (-) 339 WP_213381076.1 P-II family nitrogen regulator -
  Atep_RS06925 (Atep_13490) - 1434421..1435143 (-) 723 WP_213381077.1 TorF family putative porin -
  Atep_RS06930 (Atep_13500) ubiK 1435568..1435834 (+) 267 WP_213381078.1 ubiquinone biosynthesis accessory factor UbiK -
  Atep_RS06935 (Atep_13510) comM 1435850..1437367 (+) 1518 WP_213381079.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  Atep_RS06940 (Atep_13520) pilU 1437496..1438731 (+) 1236 WP_213381082.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Atep_RS06945 (Atep_13530) pilU 1438758..1439813 (+) 1056 WP_213381083.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Atep_RS06950 (Atep_13540) - 1439838..1440407 (-) 570 WP_213381084.1 Uma2 family endonuclease -
  Atep_RS06955 (Atep_13550) - 1440471..1440782 (-) 312 WP_213381464.1 Wadjet anti-phage system protein JetD domain-containing protein -
  Atep_RS06960 (Atep_13560) - 1440710..1441222 (-) 513 WP_213381466.1 AAA family ATPase -
  Atep_RS06965 (Atep_13570) - 1441282..1442046 (-) 765 WP_213381468.1 arginyltransferase -
  Atep_RS06970 (Atep_13580) aat 1442043..1442786 (-) 744 WP_213381088.1 leucyl/phenylalanyl-tRNA--protein transferase -
  Atep_RS06975 (Atep_13590) trxB 1442796..1443758 (-) 963 WP_213381090.1 thioredoxin-disulfide reductase -
  Atep_RS06980 (Atep_13600) - 1444064..1444543 (+) 480 WP_213381091.1 C40 family peptidase -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38713.70 Da        Isoelectric Point: 6.4307

>NTDB_id=86018 Atep_RS06945 WP_213381083.1 1438758..1439813(+) (pilU) [Allochromatium tepidum strain NZ]
MDIKPYLELMVKHKASDLFFAAGTQVKIKIEGVIRSVGETVLTAEACRAAVEGIMTPEQIKELERERELDFAIALEGKAR
FRVNAYFQRGQPAMVLRYIRANVPTIEELNVPPILSQLIMHKRGIILMVGATGSGKSTTLAAMINHRNRNSTGHIVTIED
PIEFTHTHQQCIVSQREIGIDTKSYANALKSVLREAPDVVLIGEIRTRETMEAAIELAGTGHLAISTLHANNAYQAMERI
INMFPQEMHKTLFMDLSAYMRAILSQRLVRTKDGSRCAAIEILVNTPHIADLIRDGRVDAINEAMQESSAGGMCTFDDAL
LALYNAGTITMEEALANADSAANLEAKIHFG

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=86018 Atep_RS06945 WP_213381083.1 1438758..1439813(+) (pilU) [Allochromatium tepidum strain NZ]
ATGGACATCAAGCCCTATCTCGAACTCATGGTCAAGCACAAGGCTTCGGACTTGTTCTTCGCCGCCGGCACCCAGGTCAA
GATCAAGATCGAGGGCGTGATCCGCTCGGTCGGCGAGACGGTGCTGACGGCGGAGGCCTGTCGGGCGGCGGTCGAGGGCA
TCATGACCCCCGAGCAGATCAAGGAACTGGAACGCGAGCGGGAGCTGGATTTCGCCATCGCCCTGGAAGGCAAGGCACGC
TTCCGCGTCAACGCCTATTTCCAGCGCGGTCAGCCGGCGATGGTGTTGCGCTACATCCGCGCCAATGTGCCCACGATCGA
GGAACTCAACGTCCCGCCGATCCTCTCGCAGTTGATCATGCACAAGCGCGGGATCATCCTCATGGTCGGCGCCACCGGCT
CGGGCAAGTCGACCACGCTCGCGGCCATGATCAATCATCGCAATCGCAATTCGACCGGCCACATCGTCACCATCGAGGAT
CCGATCGAGTTCACCCACACCCACCAGCAGTGCATCGTCAGCCAGCGTGAGATCGGCATCGACACCAAGAGCTATGCCAA
CGCGCTCAAGAGCGTGCTGCGCGAGGCGCCGGACGTGGTGCTGATCGGTGAGATTCGTACCCGCGAGACCATGGAGGCGG
CGATCGAGCTGGCCGGAACCGGACATCTGGCGATCTCGACCCTGCATGCCAACAATGCCTATCAAGCCATGGAACGCATC
ATCAACATGTTTCCGCAGGAAATGCACAAGACGCTGTTCATGGATCTCTCGGCCTACATGCGCGCCATCCTGTCGCAGCG
TCTGGTGCGTACCAAGGACGGCTCGCGCTGTGCGGCGATCGAGATCCTCGTCAACACGCCGCACATCGCCGACCTGATCC
GCGACGGACGGGTCGACGCCATCAACGAGGCGATGCAGGAAAGCAGTGCCGGGGGCATGTGCACCTTCGACGACGCGCTG
CTGGCGCTCTACAACGCCGGCACCATCACCATGGAAGAGGCCCTGGCGAATGCCGACTCGGCGGCCAATCTGGAGGCGAA
GATCCATTTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.557

100

0.527
  pilU Acinetobacter baylyi ADP1

51.429

99.715

0.513
  pilU Vibrio cholerae strain A1552

47.536

98.291

0.467
  pilT Neisseria meningitidis 8013

41.261

99.43

0.41
  pilT Neisseria gonorrhoeae MS11

40.805

99.145

0.405
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

96.581

0.405
  pilT Vibrio cholerae strain A1552

41.141

94.872

0.39
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

94.872

0.39
  pilT Pseudomonas aeruginosa PAK

39.535

98.006

0.387
  pilT Legionella pneumophila strain ERS1305867

40.419

95.157

0.385
  pilT Legionella pneumophila strain Lp02

40.419

95.157

0.385
  pilT Acinetobacter baumannii D1279779

39.82

95.157

0.379
  pilT Acinetobacter baumannii strain A118

39.82

95.157

0.379
  pilT Acinetobacter nosocomialis M2

39.82

95.157

0.379
  pilT Acinetobacter baylyi ADP1

38.824

96.866

0.376
  pilT Pseudomonas stutzeri DSM 10701

38.372

98.006

0.376

Multiple sequence alignment