Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Atep_RS06940 Genome accession   NZ_AP024563
Coordinates   1437496..1438731 (+) Length   411 a.a.
NCBI ID   WP_213381082.1    Uniprot ID   -
Organism   Allochromatium tepidum strain NZ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1432496..1443731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Atep_RS06915 (Atep_13470) - 1432574..1433923 (-) 1350 WP_272876312.1 ammonium transporter -
  Atep_RS06920 (Atep_13480) glnK 1433948..1434286 (-) 339 WP_213381076.1 P-II family nitrogen regulator -
  Atep_RS06925 (Atep_13490) - 1434421..1435143 (-) 723 WP_213381077.1 TorF family putative porin -
  Atep_RS06930 (Atep_13500) ubiK 1435568..1435834 (+) 267 WP_213381078.1 ubiquinone biosynthesis accessory factor UbiK -
  Atep_RS06935 (Atep_13510) comM 1435850..1437367 (+) 1518 WP_213381079.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  Atep_RS06940 (Atep_13520) pilU 1437496..1438731 (+) 1236 WP_213381082.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Atep_RS06945 (Atep_13530) pilU 1438758..1439813 (+) 1056 WP_213381083.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Atep_RS06950 (Atep_13540) - 1439838..1440407 (-) 570 WP_213381084.1 Uma2 family endonuclease -
  Atep_RS06955 (Atep_13550) - 1440471..1440782 (-) 312 WP_213381464.1 Wadjet anti-phage system protein JetD domain-containing protein -
  Atep_RS06960 (Atep_13560) - 1440710..1441222 (-) 513 WP_213381466.1 AAA family ATPase -
  Atep_RS06965 (Atep_13570) - 1441282..1442046 (-) 765 WP_213381468.1 arginyltransferase -
  Atep_RS06970 (Atep_13580) aat 1442043..1442786 (-) 744 WP_213381088.1 leucyl/phenylalanyl-tRNA--protein transferase -

Sequence

Protein


Download         Length: 411 a.a.        Molecular weight: 45100.51 Da        Isoelectric Point: 6.0121

>NTDB_id=86017 Atep_RS06940 WP_213381082.1 1437496..1438731(+) (pilU) [Allochromatium tepidum strain NZ]
MDRQQAANFMLDCLRKMTQKNGSDLFISAGFPPACKIHGRMTPMSTQALSGEQTNILVRSIMNDRQVRDFDAHKECNFAI
SPKGIGRFRVNAFVQQGLSGAVLRTINSGIPTIDELKLPPVLKEIVMNKRGLVLVVGGTGSGKSTSLAAMLGHRNANSYG
HIITIEDPVEYVHPHINCLITQREVGVDTESWDAALVNTLRQAPDVILIGEIRTRETMEHAINFAETGHLCLSTLHANSA
NQALDRIINLFPEERRGQLLMDLSLNLNAIISQRLLPCTDGGRVAAVEIMLNSPLIQDLIFKGDVGGIKAVMKKSRELGM
QTFDMALFDLYEAGKISYEDALRNADSMNGLRLAIKLEGRATREKDHFGSTDGLSILSDEDEEGGQDSSLFGESSPVSTI
EDPPTRFTPNR

Nucleotide


Download         Length: 1236 bp        

>NTDB_id=86017 Atep_RS06940 WP_213381082.1 1437496..1438731(+) (pilU) [Allochromatium tepidum strain NZ]
ATGGATCGTCAACAAGCCGCGAATTTCATGCTCGACTGTCTGCGCAAGATGACGCAGAAAAACGGGTCCGATCTCTTCAT
CTCGGCCGGATTCCCGCCCGCCTGCAAGATCCATGGGCGCATGACGCCGATGAGCACTCAGGCCCTGTCCGGCGAGCAGA
CCAATATTCTGGTGCGCTCGATCATGAACGATCGCCAGGTGCGCGACTTCGACGCGCACAAGGAGTGCAACTTCGCCATC
AGTCCCAAGGGCATCGGGCGTTTTCGCGTCAATGCCTTCGTGCAGCAGGGGCTGTCGGGCGCGGTGCTGCGCACCATCAA
CTCCGGCATCCCGACCATCGACGAGCTGAAGCTGCCGCCGGTGCTCAAAGAGATCGTGATGAACAAGCGCGGCCTGGTGC
TCGTGGTCGGCGGCACCGGCTCGGGCAAGTCGACCTCGCTCGCGGCCATGCTCGGGCATCGCAACGCCAACAGTTACGGC
CACATCATCACCATCGAAGACCCGGTCGAATACGTGCACCCGCACATCAACTGTCTGATCACGCAGCGCGAGGTCGGCGT
CGATACCGAGAGCTGGGATGCCGCGCTCGTCAACACCCTGCGTCAGGCTCCGGACGTGATCCTGATCGGCGAGATCCGCA
CCCGCGAGACCATGGAGCATGCGATCAACTTCGCCGAGACCGGACACCTCTGTCTCTCGACCCTGCACGCCAACAGTGCC
AACCAGGCGCTCGACCGCATCATCAACCTCTTCCCCGAGGAGCGCCGCGGTCAGCTCCTGATGGACCTCTCGCTCAATCT
CAACGCCATCATCTCGCAGCGTCTGCTGCCCTGCACCGACGGCGGCCGTGTCGCCGCCGTCGAGATCATGCTCAACTCGC
CGCTGATCCAGGATCTGATCTTCAAAGGCGATGTCGGCGGCATCAAGGCAGTCATGAAGAAGTCGCGCGAACTCGGGATG
CAGACCTTCGACATGGCGCTGTTCGATCTCTACGAAGCCGGCAAGATCAGCTATGAGGATGCCCTGCGCAACGCCGATTC
CATGAACGGTCTGCGTCTGGCGATCAAGCTGGAAGGACGCGCGACGCGCGAGAAGGATCACTTCGGCAGCACCGATGGCC
TGTCGATCCTGAGCGACGAGGACGAAGAGGGCGGCCAGGATTCGAGTCTGTTCGGCGAGTCCTCGCCGGTCTCGACCATC
GAAGACCCGCCGACACGCTTCACGCCGAATCGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.322

90.754

0.547
  pilU Acinetobacter baylyi ADP1

55.742

86.861

0.484
  pilU Vibrio cholerae strain A1552

53.521

86.375

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.797

83.942

0.384
  pilT Acinetobacter nosocomialis M2

45.82

78.589

0.36
  pilT Acinetobacter baumannii D1279779

45.82

78.589

0.36
  pilT Acinetobacter baumannii strain A118

45.82

78.589

0.36

Multiple sequence alignment