Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OOT55_RS11850 Genome accession   NZ_CP110629
Coordinates   2731515..2732549 (-) Length   344 a.a.
NCBI ID   WP_024461433.1    Uniprot ID   A0A3N1NZB4
Organism   Marinimicrobium sp. C6131     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2726515..2737549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOT55_RS11820 (OOT55_11820) pilG 2726577..2726972 (-) 396 WP_024461427.1 twitching motility response regulator PilG Regulator
  OOT55_RS11825 (OOT55_11825) gshB 2727378..2728325 (+) 948 WP_265366074.1 glutathione synthase -
  OOT55_RS11830 (OOT55_11830) - 2728361..2729266 (+) 906 WP_265366075.1 energy transducer TonB -
  OOT55_RS11835 (OOT55_11835) - 2729324..2729917 (+) 594 WP_265366076.1 YqgE/AlgH family protein -
  OOT55_RS11840 (OOT55_11840) ruvX 2729919..2730323 (+) 405 WP_265366077.1 Holliday junction resolvase RuvX -
  OOT55_RS11845 (OOT55_11845) pilU 2730350..2731495 (-) 1146 WP_265366078.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OOT55_RS11850 (OOT55_11850) pilT 2731515..2732549 (-) 1035 WP_024461433.1 type IV pilus twitching motility protein PilT Machinery gene
  OOT55_RS11855 (OOT55_11855) - 2732645..2733337 (+) 693 WP_265366079.1 YggS family pyridoxal phosphate-dependent enzyme -
  OOT55_RS11860 (OOT55_11860) proC 2733354..2734181 (+) 828 WP_265366080.1 pyrroline-5-carboxylate reductase -
  OOT55_RS11865 (OOT55_11865) - 2734223..2734813 (+) 591 WP_265366081.1 YggT family protein -
  OOT55_RS11870 (OOT55_11870) - 2734957..2736939 (+) 1983 WP_265366082.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38191.89 Da        Isoelectric Point: 6.6638

>NTDB_id=755457 OOT55_RS11850 WP_024461433.1 2731515..2732549(-) (pilT) [Marinimicrobium sp. C6131]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHSLIYEIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGSVFRDICAVPRGLVLVTGPTGSGKSTTLAAMMDYINDNKYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPANEKAMVRSMLSESLQAVISQTLLKKNGGGRVAAHEIMRGTSAIRNLIREDKVAQMYSAIQTGASLGMQTMDQCL
ADLVARRLVNRDVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=755457 OOT55_RS11850 WP_024461433.1 2731515..2732549(-) (pilT) [Marinimicrobium sp. C6131]
ATGGATATTACGGAACTTCTGGCCTTCAGCGCTAAACAGGGCGCTTCAGACTTGCACCTCTCCGCAGGCCTGCCCCCGAT
GATCCGGGTGGACGGCGATGTGCGGCGGATCAACCTGCCTCCCATGGAGCACAAGCAGGTGCATAGTTTGATCTACGAAA
TAATGAATGACAAGCAGCGCAAGGACTTTGAAGAGTTCCTGGAAACCGACTTCTCCTTTGAGGTGCCCGGCGTGGCCCGC
TTCCGGGTGAACGCCTTCAATCAGAACCGCGGTGCCGGTGCGGTGTTCCGGACCATTCCGTCCAAGGTGTTGACCATGGA
AGAGCTGGGTATGGGCAGCGTGTTTCGGGATATCTGCGCGGTGCCCCGGGGGCTGGTGTTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACCACTCTGGCGGCGATGATGGATTACATCAATGACAACAAGTATGAACACATTCTGACCATTGAGGAC
CCGATCGAATTTGTGCACGAAAGCAAAAAGTGCCTGGTCAACCAGCGCGAAGTGCACCGGGACACTCACGGCTTTTCGGA
AGCGCTTCGTTCAGCGCTGCGGGAAGACCCGGATATCATTCTGGTGGGCGAACTGCGAGACCTGGAAACCATCCGTCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATTGACCGGGTG
GTGGACGTGTTCCCGGCCAATGAGAAAGCCATGGTCCGCTCGATGTTGTCCGAGTCGTTGCAGGCGGTCATCTCCCAGAC
CCTGCTCAAGAAAAACGGTGGCGGCCGGGTGGCGGCACATGAAATCATGCGAGGCACCTCCGCCATTCGCAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCTGCCATCCAGACCGGGGCTTCCCTGGGGATGCAGACGATGGATCAGTGCCTG
GCGGATCTGGTAGCGCGTCGGTTGGTCAATCGCGACGTGGCCCGGGAAAAAGCCAAGATGCCGGAAAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3N1NZB4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

90.116

100

0.901
  pilT Pseudomonas stutzeri DSM 10701

89.535

100

0.895
  pilT Acinetobacter baumannii D1279779

83.43

100

0.834
  pilT Acinetobacter baumannii strain A118

83.43

100

0.834
  pilT Acinetobacter nosocomialis M2

83.14

100

0.831
  pilT Acinetobacter baylyi ADP1

80.814

100

0.808
  pilT Legionella pneumophila strain Lp02

73.964

98.256

0.727
  pilT Legionella pneumophila strain ERS1305867

73.964

98.256

0.727
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.824

98.837

0.68
  pilT Vibrio cholerae strain A1552

68.824

98.837

0.68
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Pseudomonas stutzeri DSM 10701

40.979

95.058

0.39
  pilU Vibrio cholerae strain A1552

41.231

94.477

0.39
  pilU Acinetobacter baylyi ADP1

37.861

100

0.381