Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FGKAn22_RS11410 Genome accession   NZ_AP019536
Coordinates   2348151..2349194 (-) Length   347 a.a.
NCBI ID   WP_212785754.1    Uniprot ID   A0AAN1T0S7
Organism   Ferrigenium kumadai strain An22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2343151..2354194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGKAn22_RS11385 (FGKAn22_22110) - 2343358..2344272 (+) 915 WP_212785749.1 ATP-binding cassette domain-containing protein -
  FGKAn22_RS11390 (FGKAn22_22120) - 2344286..2345071 (+) 786 WP_212785750.1 ABC transporter permease -
  FGKAn22_RS11395 (FGKAn22_22130) hemJ 2345262..2345672 (+) 411 WP_212785751.1 protoporphyrinogen oxidase HemJ -
  FGKAn22_RS11400 (FGKAn22_22140) - 2345676..2346815 (+) 1140 WP_212785752.1 THUMP domain-containing protein -
  FGKAn22_RS11405 (FGKAn22_22150) pilU 2346858..2347994 (-) 1137 WP_212785753.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FGKAn22_RS11410 (FGKAn22_22160) pilT 2348151..2349194 (-) 1044 WP_212785754.1 type IV pilus twitching motility protein PilT Machinery gene
  FGKAn22_RS11415 (FGKAn22_22170) - 2349254..2349952 (+) 699 WP_212785755.1 YggS family pyridoxal phosphate-dependent enzyme -
  FGKAn22_RS11420 (FGKAn22_22180) proC 2349949..2350761 (+) 813 WP_212785756.1 pyrroline-5-carboxylate reductase -
  FGKAn22_RS11425 (FGKAn22_22190) - 2350758..2351327 (+) 570 WP_212785757.1 YggT family protein -
  FGKAn22_RS11430 (FGKAn22_22200) - 2351329..2351616 (+) 288 WP_212785758.1 DUF167 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38162.17 Da        Isoelectric Point: 7.0223

>NTDB_id=72684 FGKAn22_RS11410 WP_212785754.1 2348151..2349194(-) (pilT) [Ferrigenium kumadai strain An22]
MDITELLAFGVKNKASDLHLSAGLPPMIRVHGDMRRINLPAMEHKEVHAMVYDIMNDGQRKFYEENKEVDFSFEVPNLAR
FRVNAFVQQRGAAAVMRTIPSKVLTLEDLKAPKIFTDISQFPRGLVLVTGPTGSGKSTTLAAMINFINETELGHILTVED
PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPADEKEMVRAMLSESLRAVISQALLKTKDGSGRVAAHEIMLCTPAIRNLIREAKVPQMYSAIQTGQSMGMQTLDQC
LTNLVKGGVVSAAEARGKAANKDLFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=72684 FGKAn22_RS11410 WP_212785754.1 2348151..2349194(-) (pilT) [Ferrigenium kumadai strain An22]
ATGGATATCACCGAACTGCTCGCCTTCGGCGTCAAGAACAAGGCTTCCGACCTGCATCTTTCCGCCGGTCTGCCGCCGAT
GATCCGCGTGCACGGCGATATGCGCCGCATCAATTTGCCCGCGATGGAGCACAAGGAAGTCCACGCCATGGTGTACGACA
TCATGAACGACGGGCAGCGCAAGTTCTACGAAGAGAACAAGGAAGTCGACTTCTCCTTCGAAGTGCCTAACCTAGCGCGT
TTCCGCGTGAACGCCTTCGTGCAGCAGCGCGGCGCGGCAGCGGTGATGCGTACCATCCCATCCAAGGTGCTGACGCTCGA
AGACCTCAAGGCGCCGAAGATCTTCACCGACATCTCCCAATTCCCCCGCGGGCTGGTGCTGGTGACCGGCCCGACCGGTT
CCGGTAAGTCCACCACGCTGGCGGCGATGATCAACTTCATCAATGAAACCGAACTGGGCCATATCCTGACCGTGGAAGAC
CCGATCGAATTCGTGCACGAGTCGAAGAAGTGTCTGATCAACCAGCGCGAAGTCGGCCCGCACACGCTGTCGTTCAACAA
CGCGCTGCGTTCCGCGCTGCGCGAAGACCCGGACGTGATCCTCGTCGGCGAAATGCGCGATCTGGAAACCATCCGCCTCG
CGATGACCGCGGCGGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCGGCGGACGAAAAGGAAATGGTGCGCGCGATGCTGTCCGAATCGCTGCGTGCAGTGATCTCGCAGGC
GTTGCTCAAGACCAAGGACGGTTCCGGACGAGTCGCCGCGCACGAGATCATGCTCTGTACCCCCGCGATCCGCAACCTGA
TCCGCGAAGCCAAGGTGCCGCAGATGTATTCCGCGATCCAGACCGGCCAGTCTATGGGCATGCAGACGCTCGATCAATGC
CTGACCAATCTGGTGAAGGGCGGTGTGGTGTCTGCGGCGGAAGCGCGTGGCAAGGCGGCGAACAAGGATCTGTTCCCCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.424

0.741
  pilT Pseudomonas aeruginosa PAK

75.516

97.695

0.738
  pilT Legionella pneumophila strain Lp02

73.333

99.424

0.729
  pilT Legionella pneumophila strain ERS1305867

73.333

99.424

0.729
  pilT Neisseria meningitidis 8013

72.886

98.847

0.72
  pilT Neisseria gonorrhoeae MS11

72.595

98.847

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.935

97.406

0.671
  pilT Vibrio cholerae strain A1552

68.935

97.406

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.681

93.948

0.504
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409
  pilU Vibrio cholerae strain A1552

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

40.179

96.83

0.389

Multiple sequence alignment