Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FGKAn22_RS11405 Genome accession   NZ_AP019536
Coordinates   2346858..2347994 (-) Length   378 a.a.
NCBI ID   WP_212785753.1    Uniprot ID   A0AAN1T140
Organism   Ferrigenium kumadai strain An22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2341858..2352994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGKAn22_RS11375 (FGKAn22_22090) - 2342430..2342741 (+) 312 WP_212785747.1 NAD(P)H-dependent oxidoreductase subunit E -
  FGKAn22_RS11380 (FGKAn22_22100) - 2342741..2343361 (+) 621 WP_212785748.1 alpha/beta fold hydrolase -
  FGKAn22_RS11385 (FGKAn22_22110) - 2343358..2344272 (+) 915 WP_212785749.1 ATP-binding cassette domain-containing protein -
  FGKAn22_RS11390 (FGKAn22_22120) - 2344286..2345071 (+) 786 WP_212785750.1 ABC transporter permease -
  FGKAn22_RS11395 (FGKAn22_22130) hemJ 2345262..2345672 (+) 411 WP_212785751.1 protoporphyrinogen oxidase HemJ -
  FGKAn22_RS11400 (FGKAn22_22140) - 2345676..2346815 (+) 1140 WP_212785752.1 THUMP domain-containing protein -
  FGKAn22_RS11405 (FGKAn22_22150) pilU 2346858..2347994 (-) 1137 WP_212785753.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FGKAn22_RS11410 (FGKAn22_22160) pilT 2348151..2349194 (-) 1044 WP_212785754.1 type IV pilus twitching motility protein PilT Machinery gene
  FGKAn22_RS11415 (FGKAn22_22170) - 2349254..2349952 (+) 699 WP_212785755.1 YggS family pyridoxal phosphate-dependent enzyme -
  FGKAn22_RS11420 (FGKAn22_22180) proC 2349949..2350761 (+) 813 WP_212785756.1 pyrroline-5-carboxylate reductase -
  FGKAn22_RS11425 (FGKAn22_22190) - 2350758..2351327 (+) 570 WP_212785757.1 YggT family protein -
  FGKAn22_RS11430 (FGKAn22_22200) - 2351329..2351616 (+) 288 WP_212785758.1 DUF167 domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42167.61 Da        Isoelectric Point: 6.6392

>NTDB_id=72683 FGKAn22_RS11405 WP_212785753.1 2346858..2347994(-) (pilU) [Ferrigenium kumadai strain An22]
MERDQATELIHNLLRGMISKKASDLFITAGFPPAFKVDGKMTPVSNQALTAQHTQELVRSIMNDRQAAEFEATHECNFAI
SPPGIGRFRVNVFMQQQRVGMVLRTITTKIPDLDAMGLPPVLKDIVMTKRGLVILVGATGSGKSTTLAAMLGHRNRNSYG
HIITIEDPVEYVHEHANCIITHREVGVDTENWHNALKNTLRQAPDVILIGEIRDRETMEYAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERREQLLMDLSLNVKSLISQRLIPKKDGVGRAAAMEILLNSPLIADLIFKGEVHAIKGVMAKSRELG
MQTFDQALFDLFEEDKISYEEALKNADSVNDLRLRIKLESKNAMDRDVMGGTENLKLT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=72683 FGKAn22_RS11405 WP_212785753.1 2346858..2347994(-) (pilU) [Ferrigenium kumadai strain An22]
ATGGAAAGAGACCAAGCCACCGAGCTTATCCATAACCTGCTGCGCGGTATGATCAGCAAGAAGGCATCCGACTTGTTCAT
CACCGCCGGCTTTCCGCCTGCGTTCAAGGTGGACGGCAAGATGACGCCGGTCTCCAATCAGGCGCTGACGGCACAGCACA
CTCAGGAACTGGTGCGAAGCATCATGAACGACCGCCAGGCGGCCGAGTTCGAGGCGACGCACGAGTGCAACTTCGCCATC
AGTCCGCCCGGCATCGGGCGCTTCCGCGTCAACGTGTTCATGCAGCAGCAGCGCGTCGGCATGGTGCTGCGTACCATCAC
CACCAAGATCCCAGATCTCGACGCGATGGGGCTGCCGCCGGTGCTGAAGGACATCGTGATGACCAAGCGTGGCTTGGTGA
TCCTGGTCGGCGCCACCGGCTCGGGCAAATCCACCACGCTCGCGGCGATGCTGGGCCACCGCAACAGGAACAGTTACGGC
CACATCATCACCATCGAAGACCCGGTGGAATACGTGCACGAGCACGCCAACTGCATCATCACCCACCGCGAGGTCGGCGT
GGATACGGAGAACTGGCACAATGCGCTGAAGAACACCCTGCGCCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGCG
ACCGCGAGACCATGGAATACGCCGTCGCCTTTGCCGAGACCGGCCACCTGTGTATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCGGAAGAGCGGCGTGAGCAACTGCTGATGGACCTCTCGCTCAACGT
CAAATCGCTGATCTCGCAACGTCTGATCCCGAAGAAGGACGGCGTCGGACGCGCCGCCGCGATGGAGATCTTGCTCAACT
CGCCGCTGATCGCAGACCTGATCTTCAAGGGCGAGGTGCATGCCATCAAGGGCGTGATGGCCAAGTCGCGCGAGCTGGGC
ATGCAGACCTTCGATCAGGCATTGTTCGACCTGTTCGAGGAGGACAAGATCTCCTACGAAGAGGCGTTGAAGAACGCCGA
CTCGGTCAACGACCTGCGCCTGCGCATCAAGCTGGAGAGCAAGAACGCGATGGACCGCGATGTGATGGGCGGTACAGAGA
ATCTGAAGCTCACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.037

92.328

0.582
  pilU Acinetobacter baylyi ADP1

60.286

92.593

0.558
  pilU Vibrio cholerae strain A1552

57.184

92.063

0.526
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

42.773

89.683

0.384
  pilT Acinetobacter nosocomialis M2

42.943

88.095

0.378
  pilT Acinetobacter baumannii D1279779

42.943

88.095

0.378
  pilT Acinetobacter baumannii strain A118

42.943

88.095

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.136

89.153

0.376

Multiple sequence alignment