Detailed information NTG0070503

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: EP379_RS00115
• Genome accession: NZ_AP018724
• Coordinates: 26027..27064 (-)
• Length: 345 a.a.
• NCBI ID: WP_127474531.1
• Uniprot ID: -
• Organism: Sulfurivermis fontis strain JG42
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 21027..32064

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EP379_RS00095	-	21296..22294 (+)	999	WP_172600321.1	hypothetical protein	-
EP379_RS00100	-	22308..24431 (+)	2124	WP_127474525.1	putative bifunctional diguanylate cyclase/phosphodiesterase	-
EP379_RS00110	pilU	24849..26006 (-)	1158	WP_127474529.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EP379_RS00115	pilT	26027..27064 (-)	1038	WP_127474531.1	type IV pilus twitching motility protein PilT	Machinery gene
EP379_RS00120	-	27156..27860 (+)	705	WP_127474533.1	YggS family pyridoxal phosphate-dependent enzyme	-
EP379_RS00125	proC	27905..28735 (+)	831	WP_127474535.1	pyrroline-5-carboxylate reductase	-
EP379_RS00130	-	28732..29313 (+)	582	WP_127474536.1	YggT family protein	-
EP379_RS00135	-	29310..29603 (+)	294	WP_127474538.1	DUF167 family protein	-
EP379_RS00140	-	29724..31694 (+)	1971	WP_127474540.1	dynamin family protein	-

Sequence

Protein

• Length: 345 a.a.
• Molecular weight: 38256.94 Da
• Isoelectric Point: 6.8399

>NTDB_id=70503 EP379_RS00115 WP_127474531.1 26027..27064(-) (pilT) [Sulfurivermis fontis strain JG42]
MDITELLAFGVKNGASDLHLSAGLPPMIRVDGDVRRINVPPLEHKQVHGMVYDIMNDKQRKDFEEFLETDFSFEIKGLAR
FRVNAFNQNRGAGAVFRTIPSKILSLEELNAPPIFKEISDNPRGIVLVTGPTGSGKSTTLAAMIDYKNNTEYGHILTVED
PIEFVHESKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRSMLSESLRAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIRESKIAQMYSAIQTGQQYGMQTLDQNL
IELVQRGIVSKQEAAHKAANKDAFK

Nucleotide

• Length: 1038 bp

>NTDB_id=70503 EP379_RS00115 WP_127474531.1 26027..27064(-) (pilT) [Sulfurivermis fontis strain JG42]
ATGGATATTACCGAACTTCTCGCCTTCGGGGTCAAGAACGGCGCGTCAGACCTGCATCTCTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGCATCAACGTACCGCCGCTGGAGCACAAACAGGTGCACGGCATGGTGTACGACA
TCATGAACGACAAGCAACGCAAGGACTTCGAGGAATTCCTCGAGACCGACTTCTCGTTCGAGATCAAGGGACTGGCCCGT
TTCCGCGTCAACGCCTTCAACCAGAACCGTGGTGCCGGTGCGGTGTTCCGTACCATTCCCTCCAAGATCCTCAGCCTGGA
AGAACTGAACGCGCCGCCGATCTTCAAGGAAATTTCCGACAACCCGCGCGGCATCGTGCTGGTCACCGGCCCGACCGGCT
CCGGCAAGTCCACCACCCTGGCGGCAATGATCGACTACAAGAACAACACCGAATACGGTCACATTCTCACCGTCGAGGAC
CCCATCGAATTCGTGCACGAATCCAAGAAATGTCTCATCAACCAGCGCGAGGTGCACCGCGATACCCTCGGCTTCAACGA
AGCCCTGCGTTCGGCGCTGCGCGAAGATCCCGACATCATCCTCGTCGGCGAAATGCGTGACCTGGAAACCATCCGTCTGG
CCCTGACCGCAGCCGAAACCGGCCATCTGGTATTCGGCACCCTGCACACCAGCTCGGCTGCCAAGACCATCGACCGTATC
ATCGACGTTTTCCCGGCGGCGGAAAAGGACATGGTACGTTCGATGCTGTCCGAATCCCTGCGCGCGGTTATCTCACAGAC
GCTGCTGAAGAAGATCGGCGGTGGCCGTGTGGCGGCCCACGAAATCATGATCGGCACACCGGCCATTCGCAACCTCATCC
GCGAATCCAAGATTGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAATACGGTATGCAGACGCTGGATCAGAACCTG
ATTGAACTGGTGCAGCGCGGCATCGTCAGCAAGCAGGAAGCCGCCCACAAGGCTGCCAACAAAGACGCCTTCAAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (11-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Pseudomonas aeruginosa PAK	82.558	99.71	0.823
pilT	Acinetobacter nosocomialis M2	81.686	99.71	0.814
pilT	Acinetobacter baumannii D1279779	81.686	99.71	0.814
pilT	Acinetobacter baumannii strain A118	81.686	99.71	0.814
pilT	Pseudomonas stutzeri DSM 10701	80.523	99.71	0.803
pilT	Acinetobacter baylyi ADP1	79.36	99.71	0.791
pilT	Legionella pneumophila strain Lp02	77.035	99.71	0.768
pilT	Legionella pneumophila strain ERS1305867	77.035	99.71	0.768
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	70.03	97.681	0.684
pilT	Vibrio cholerae strain A1552	70.03	97.681	0.684
pilT	Neisseria meningitidis 8013	68.406	100	0.684
pilT	Neisseria gonorrhoeae MS11	68.116	100	0.681
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	50.435	100	0.504
pilU	Acinetobacter baylyi ADP1	41.471	98.551	0.409
pilU	Vibrio cholerae strain A1552	41.298	98.261	0.406
pilU	Pseudomonas stutzeri DSM 10701	41.194	97.101	0.4
pilB	Acinetobacter baumannii D1279779	32.552	100	0.362