Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VCASEI_RS03015 Genome accession   NZ_AP018680
Coordinates   612783..613820 (+) Length   345 a.a.
NCBI ID   WP_086961676.1    Uniprot ID   -
Organism   Vibrio casei strain DSM 22364     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 607783..618820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCASEI_RS02980 hemW 607796..608968 (-) 1173 WP_086961691.1 radical SAM family heme chaperone HemW -
  VCASEI_RS02985 - 608978..609586 (-) 609 WP_086961689.1 XTP/dITP diphosphatase -
  VCASEI_RS02990 - 609613..610044 (-) 432 WP_086961687.1 DUF4426 domain-containing protein -
  VCASEI_RS02995 yggU 610081..610377 (-) 297 WP_086961685.1 DUF167 family protein YggU -
  VCASEI_RS03000 - 610415..610969 (-) 555 WP_086961683.1 YggT family protein -
  VCASEI_RS03005 proC 611020..611838 (-) 819 WP_086961681.1 pyrroline-5-carboxylate reductase -
  VCASEI_RS03010 - 612020..612757 (-) 738 WP_086961679.1 YggS family pyridoxal phosphate-dependent enzyme -
  VCASEI_RS03015 pilT 612783..613820 (+) 1038 WP_086961676.1 type IV pilus twitching motility protein PilT Machinery gene
  VCASEI_RS03020 pilU 613849..614952 (+) 1104 WP_086961674.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VCASEI_RS03025 - 615050..615997 (-) 948 WP_086961673.1 phytanoyl-CoA dioxygenase family protein -
  VCASEI_RS03030 - 616174..617082 (+) 909 WP_086961671.1 AraC family transcriptional regulator -
  VCASEI_RS03035 ruvX 617079..617501 (-) 423 WP_086961669.1 Holliday junction resolvase RuvX -
  VCASEI_RS03040 - 617548..618111 (-) 564 WP_086961667.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38447.03 Da        Isoelectric Point: 5.7623

>NTDB_id=70313 VCASEI_RS03015 WP_086961676.1 612783..613820(+) (pilT) [Vibrio casei strain DSM 22364]
MDIAELLDFSVKHNASDLHLSAGVPPMLRIDGDMRKLEMDAFNHAEVHALIFDIMNDAQRSEYEEKLEIDFSFELPNVGR
FRVNAFNQSRGCAAVFRTIPMTIPSFEDISAPEIFEKISNYEKGLVLVTGPTGSGKSTTLAAMVDYINRNHKKHILTIED
PIEFTHPNKKCLVNQREVHRDTHSFEASLRSALREDPDVILVGELRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGADKDMVRSMMSESLRAVIAQKLLKRNGGGRVACHEVMIATPAIRNLIREDKVAQMLSMIQTGSATGMQTMEQAA
RQLLMQGLVDQEEVQQKIETLASPY

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=70313 VCASEI_RS03015 WP_086961676.1 612783..613820(+) (pilT) [Vibrio casei strain DSM 22364]
ATGGATATCGCTGAATTATTAGATTTTAGTGTAAAGCATAATGCCTCAGATCTACATCTTTCTGCTGGCGTTCCACCAAT
GTTACGCATTGATGGAGATATGAGAAAGTTAGAAATGGATGCATTTAATCATGCTGAGGTGCATGCATTGATTTTTGATA
TTATGAACGATGCTCAGCGCAGTGAATATGAAGAAAAGCTAGAAATCGATTTTTCCTTTGAATTACCGAATGTTGGGCGA
TTTCGTGTTAATGCATTTAACCAGTCTCGCGGGTGCGCAGCGGTGTTTCGAACCATCCCGATGACCATCCCGTCATTTGA
GGACATTTCAGCGCCTGAAATATTTGAAAAAATTTCTAACTATGAAAAAGGGTTAGTATTAGTAACAGGGCCGACCGGTT
CAGGGAAATCGACGACTTTGGCTGCTATGGTTGACTACATAAATCGTAACCATAAAAAGCACATTCTCACCATTGAAGAT
CCAATAGAGTTTACCCACCCAAATAAAAAGTGTTTGGTCAACCAACGTGAAGTACATCGTGATACGCATAGTTTTGAAGC
ATCATTACGTTCGGCTTTACGTGAAGATCCTGATGTAATTCTGGTTGGAGAACTGCGTGATAAAGAAACCATTAGTCTTG
CATTGACTGCAGCCGAGACTGGGCACTTGGTATTTGGCACTTTGCACACCAGTTCAGCAGCAAAAACCATCGATCGTATT
ATTGATGTTTTTCCTGGCGCAGATAAAGATATGGTGCGTTCAATGATGTCTGAGTCTCTACGGGCTGTTATTGCACAAAA
ATTGTTGAAGCGTAATGGTGGCGGTCGAGTCGCTTGTCATGAAGTGATGATTGCGACACCAGCTATCCGTAACTTGATTC
GTGAAGATAAAGTCGCGCAAATGTTATCTATGATCCAAACCGGCTCAGCGACCGGAATGCAAACGATGGAGCAAGCGGCT
CGCCAGTTATTGATGCAAGGTTTGGTTGATCAAGAAGAAGTGCAGCAAAAAATTGAAACGTTGGCATCGCCATATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

83.481

98.261

0.82
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

83.481

98.261

0.82
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Acinetobacter nosocomialis M2

69.909

95.362

0.667
  pilT Acinetobacter baumannii D1279779

69.909

95.362

0.667
  pilT Acinetobacter baumannii strain A118

69.909

95.362

0.667
  pilT Acinetobacter baylyi ADP1

69.394

95.652

0.664
  pilT Pseudomonas aeruginosa PAK

67.359

97.681

0.658
  pilT Legionella pneumophila strain Lp02

68.278

95.942

0.655
  pilT Legionella pneumophila strain ERS1305867

68.278

95.942

0.655
  pilT Neisseria meningitidis 8013

62.941

98.551

0.62
  pilT Neisseria gonorrhoeae MS11

62.647

98.551

0.617
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.258

92.174

0.472
  pilU Vibrio cholerae strain A1552

41.317

96.812

0.4
  pilU Pseudomonas stutzeri DSM 10701

41.018

96.812

0.397
  pilU Acinetobacter baylyi ADP1

40.498

93.043

0.377

Multiple sequence alignment