Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VCASEI_RS03020 Genome accession   NZ_AP018680
Coordinates   613849..614952 (+) Length   367 a.a.
NCBI ID   WP_086961674.1    Uniprot ID   -
Organism   Vibrio casei strain DSM 22364     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 608849..619952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCASEI_RS02985 - 608978..609586 (-) 609 WP_086961689.1 XTP/dITP diphosphatase -
  VCASEI_RS02990 - 609613..610044 (-) 432 WP_086961687.1 DUF4426 domain-containing protein -
  VCASEI_RS02995 yggU 610081..610377 (-) 297 WP_086961685.1 DUF167 family protein YggU -
  VCASEI_RS03000 - 610415..610969 (-) 555 WP_086961683.1 YggT family protein -
  VCASEI_RS03005 proC 611020..611838 (-) 819 WP_086961681.1 pyrroline-5-carboxylate reductase -
  VCASEI_RS03010 - 612020..612757 (-) 738 WP_086961679.1 YggS family pyridoxal phosphate-dependent enzyme -
  VCASEI_RS03015 pilT 612783..613820 (+) 1038 WP_086961676.1 type IV pilus twitching motility protein PilT Machinery gene
  VCASEI_RS03020 pilU 613849..614952 (+) 1104 WP_086961674.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VCASEI_RS03025 - 615050..615997 (-) 948 WP_086961673.1 phytanoyl-CoA dioxygenase family protein -
  VCASEI_RS03030 - 616174..617082 (+) 909 WP_086961671.1 AraC family transcriptional regulator -
  VCASEI_RS03035 ruvX 617079..617501 (-) 423 WP_086961669.1 Holliday junction resolvase RuvX -
  VCASEI_RS03040 - 617548..618111 (-) 564 WP_086961667.1 YqgE/AlgH family protein -
  VCASEI_RS03045 gshB 618215..619165 (-) 951 WP_086961665.1 glutathione synthase -
  VCASEI_RS03050 rsmE 619201..619932 (-) 732 WP_086961663.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 41140.32 Da        Isoelectric Point: 6.5673

>NTDB_id=70314 VCASEI_RS03020 WP_086961674.1 613849..614952(+) (pilU) [Vibrio casei strain DSM 22364]
MQLSQVLEIMVSSKASDIYLTVDAPCLFRINGELKAHGEKLDKGGVEALLFEMMDADRLTEFQNQHEANFAIMRPSGRFR
VSAFFQRELPGAVIRRIETRIPSMDELHLPEVLKELATSKRGLVLVVGATGSGKSTSMAAMTGYRNLNQTGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRDTMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVAQQLVRDKNGHGRHGVFEVLLNTPRVSDLIRRGDLHELKPTMMRSKESGMQTFDQALF
DLLKADKISLDDAMHSADSANDLRLMLKSTGDSSFESNKLKGIKIDM

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=70314 VCASEI_RS03020 WP_086961674.1 613849..614952(+) (pilU) [Vibrio casei strain DSM 22364]
ATGCAACTCTCTCAAGTTTTAGAAATCATGGTGTCATCAAAAGCGTCGGATATTTATCTTACCGTGGATGCGCCTTGTTT
ATTTCGTATTAACGGTGAACTGAAAGCACATGGTGAAAAACTCGATAAAGGTGGAGTCGAAGCCCTGTTGTTTGAAATGA
TGGACGCCGACCGTCTCACTGAATTTCAAAACCAACATGAAGCTAACTTTGCAATTATGCGCCCAAGTGGGCGGTTTAGG
GTGAGCGCTTTTTTCCAACGGGAGCTTCCAGGTGCGGTGATTCGACGTATCGAAACCCGTATTCCAAGTATGGATGAATT
GCACTTACCTGAAGTGTTAAAAGAGTTAGCGACCTCAAAGCGCGGTTTGGTGCTGGTGGTTGGGGCGACAGGTTCGGGTA
AATCGACATCAATGGCAGCCATGACTGGATATCGAAATCTGAACCAAACTGGGCATATTCTTACCGTAGAAGACCCGATT
GAATTTGTGCATGAGCATAAAAAGTGCATTGTTACTCAACGTGAAGTGGGTTTAGATACAGAAAGTTATGAAGTGGCATT
AAAGAACTCGCTTCGCCAAGCACCGGATATGATTTTGATTGGTGAAATTCGTAGCCGCGATACCATGGAATATGCCATGA
CCTTTGCCGAAACTGGGCATTTGTGCATGGCTACTCTGCATGCCAATAATGCCAACCAAGCATTAGAGCGCATATTGCAC
TTGGTTCCGAAAGATCAAAAAGAGCAGTTTTTATTTGATTTATCTATGAACCTAAAAGGGGTCGTAGCCCAACAATTGGT
TCGTGACAAAAATGGTCATGGTCGCCATGGTGTGTTTGAAGTTTTGCTTAATACTCCTCGCGTTTCAGATTTGATTCGTC
GAGGTGATTTACATGAGTTAAAACCAACAATGATGCGTTCAAAAGAGTCGGGTATGCAAACCTTTGATCAAGCATTATTT
GATTTACTCAAAGCTGATAAAATTTCATTGGATGATGCGATGCACAGTGCTGATTCTGCTAATGATTTACGTTTAATGCT
TAAGTCGACGGGGGATTCATCCTTTGAAAGCAACAAGCTAAAAGGTATTAAGATTGATATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

76.567

100

0.766
  pilU Pseudomonas stutzeri DSM 10701

54.987

100

0.556
  pilU Acinetobacter baylyi ADP1

55.556

95.64

0.531
  pilT Legionella pneumophila strain Lp02

40.708

92.371

0.376
  pilT Legionella pneumophila strain ERS1305867

40.708

92.371

0.376
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.413

92.371

0.373

Multiple sequence alignment