Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JN531_RS09855 Genome accession   NZ_CP092104
Coordinates   2110100..2111134 (+) Length   344 a.a.
NCBI ID   WP_228348704.1    Uniprot ID   -
Organism   Flagellatimonas centrodinii strain R2A-3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2105100..2116134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN531_RS09830 (JN531_009830) - 2105395..2106249 (-) 855 WP_228348699.1 mechanosensitive ion channel domain-containing protein -
  JN531_RS09835 (JN531_009835) - 2106441..2106815 (-) 375 WP_366522427.1 DUF4399 domain-containing protein -
  JN531_RS09840 (JN531_009840) - 2107233..2108558 (+) 1326 WP_228348701.1 DEAD/DEAH box helicase -
  JN531_RS09845 (JN531_009845) rpiA 2108628..2109290 (-) 663 WP_228348702.1 ribose-5-phosphate isomerase RpiA -
  JN531_RS09850 (JN531_009850) - 2109287..2110000 (-) 714 WP_228348703.1 YggS family pyridoxal phosphate-dependent enzyme -
  JN531_RS09855 (JN531_009855) pilT 2110100..2111134 (+) 1035 WP_228348704.1 type IV pilus twitching motility protein PilT Machinery gene
  JN531_RS09860 (JN531_009860) pilU 2111163..2112329 (+) 1167 WP_275591450.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JN531_RS09865 (JN531_009865) pilU 2112329..2113390 (+) 1062 WP_228348705.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JN531_RS09870 (JN531_009870) rplM 2113576..2114004 (+) 429 WP_228348706.1 50S ribosomal protein L13 -
  JN531_RS09875 (JN531_009875) rpsI 2114017..2114424 (+) 408 WP_228348707.1 30S ribosomal protein S9 -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38243.03 Da        Isoelectric Point: 7.3207

>NTDB_id=655879 JN531_RS09855 WP_228348704.1 2110100..2111134(+) (pilT) [Flagellatimonas centrodinii strain R2A-3]
MDIAQLLTFGVKQGASDLHLSAGVEPMIRVDGDVKRINLPPLEHKQVHDMVYDIMNDKQRKAYDEFLETDFSFEIPGLAR
FRVNAFNHARGSGAVFRTIPSKIMSLEDLHCPPIFKDLADTPRGLILVTGPTGSGKSTTLAAMVNHKNESEYGHILTIED
PIEFVHQSKKCLINQREVHRDTLGFSEALRSALREDPDTVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPSAEKEMVRAMLSEALRAVVSQTLLKKKGGGRIAAHEIMIGTPAIRNLIRENKVAQMYSSIQTGQRDGMQTLEQCL
RDLVKRGMITPEDARRKANKPLDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=655879 JN531_RS09855 WP_228348704.1 2110100..2111134(+) (pilT) [Flagellatimonas centrodinii strain R2A-3]
ATGGATATCGCGCAACTGCTGACCTTCGGTGTCAAGCAGGGGGCCTCGGACCTTCACCTGTCCGCGGGGGTCGAGCCGAT
GATCCGGGTCGACGGGGACGTCAAGCGCATCAACCTGCCGCCGCTGGAGCACAAGCAGGTGCACGACATGGTGTACGACA
TCATGAACGACAAGCAGCGCAAGGCCTACGACGAGTTCCTGGAGACCGATTTTTCCTTCGAGATCCCGGGGCTGGCGCGT
TTCCGCGTCAACGCCTTCAACCATGCCCGCGGCTCGGGGGCGGTGTTTCGTACCATTCCTTCCAAGATCATGAGCCTGGA
GGATCTGCACTGCCCGCCGATCTTCAAGGACCTTGCCGACACGCCGCGCGGCCTGATTCTGGTCACCGGGCCGACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTGAACCACAAGAACGAAAGTGAGTACGGGCACATCCTCACTATCGAGGAC
CCGATCGAATTCGTTCACCAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGCTCGGCTTTTCCGA
GGCCCTGCGCAGCGCCCTGCGCGAGGACCCCGACACCGTGCTGGTGGGGGAAATGCGTGACCTCGAAACCATCCGTCTGG
CGTTGACGGCGGCGGAGACCGGGCACCTCGTGTTCGGCACCCTGCATACCAGCTCGGCGGCGAAGACCATCGACCGTATC
GTCGACGTCTTTCCGTCGGCCGAGAAGGAAATGGTGCGGGCGATGCTGTCGGAGGCGCTGCGGGCGGTGGTGTCGCAAAC
CCTGCTGAAGAAGAAGGGCGGTGGCCGCATCGCGGCGCATGAAATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GTGAGAACAAGGTGGCGCAGATGTATTCGTCGATTCAGACCGGGCAGCGCGATGGCATGCAGACGCTCGAACAGTGCCTG
CGCGACCTCGTCAAGCGCGGCATGATCACGCCCGAGGATGCCCGACGCAAGGCCAACAAGCCGCTGGATATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.419

99.128

0.767
  pilT Acinetobacter nosocomialis M2

77.126

99.128

0.765
  pilT Acinetobacter baumannii D1279779

77.126

99.128

0.765
  pilT Acinetobacter baumannii strain A118

77.126

99.128

0.765
  pilT Pseudomonas stutzeri DSM 10701

76.246

99.128

0.756
  pilT Acinetobacter baylyi ADP1

73.607

99.128

0.73
  pilT Legionella pneumophila strain Lp02

72.012

99.709

0.718
  pilT Legionella pneumophila strain ERS1305867

72.012

99.709

0.718
  pilT Neisseria meningitidis 8013

68.142

98.547

0.672
  pilT Neisseria gonorrhoeae MS11

67.847

98.547

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.359

97.965

0.66
  pilT Vibrio cholerae strain A1552

67.359

97.965

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.415

99.419

0.491
  pilU Pseudomonas stutzeri DSM 10701

41.449

100

0.416
  pilU Acinetobacter baylyi ADP1

41.108

99.709

0.41
  pilU Vibrio cholerae strain A1552

38.841

100

0.39