Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JN531_RS09860 Genome accession   NZ_CP092104
Coordinates   2111163..2112329 (+) Length   388 a.a.
NCBI ID   WP_275591450.1    Uniprot ID   -
Organism   Flagellatimonas centrodinii strain R2A-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2106163..2117329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN531_RS09835 (JN531_009835) - 2106441..2106815 (-) 375 WP_366522427.1 DUF4399 domain-containing protein -
  JN531_RS09840 (JN531_009840) - 2107233..2108558 (+) 1326 WP_228348701.1 DEAD/DEAH box helicase -
  JN531_RS09845 (JN531_009845) rpiA 2108628..2109290 (-) 663 WP_228348702.1 ribose-5-phosphate isomerase RpiA -
  JN531_RS09850 (JN531_009850) - 2109287..2110000 (-) 714 WP_228348703.1 YggS family pyridoxal phosphate-dependent enzyme -
  JN531_RS09855 (JN531_009855) pilT 2110100..2111134 (+) 1035 WP_228348704.1 type IV pilus twitching motility protein PilT Machinery gene
  JN531_RS09860 (JN531_009860) pilU 2111163..2112329 (+) 1167 WP_275591450.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JN531_RS09865 (JN531_009865) pilU 2112329..2113390 (+) 1062 WP_228348705.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JN531_RS09870 (JN531_009870) rplM 2113576..2114004 (+) 429 WP_228348706.1 50S ribosomal protein L13 -
  JN531_RS09875 (JN531_009875) rpsI 2114017..2114424 (+) 408 WP_228348707.1 30S ribosomal protein S9 -
  JN531_RS09885 (JN531_009885) - 2114621..2116702 (+) 2082 WP_228348708.1 S46 family peptidase -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43418.92 Da        Isoelectric Point: 6.5151

>NTDB_id=655880 JN531_RS09860 WP_275591450.1 2111163..2112329(+) (pilU) [Flagellatimonas centrodinii strain R2A-3]
MERDQAVKMMQQLLNLMKEKGGSDLFITSGFPPALKINGQMTPVGDKALPPEAAAIMMRALMNDRQTKEFEATNECNFAI
SPPNVGRFRVNAFVQQGRVGGVLRTIQTEIPSFEQLRLPARLQDIVMEKRGLVLMVGGTGSGKSTSLAAMVGYRNQQSRG
HIITIEDPVEYVHPHGGCIVTQREVGADTQSWDNALKNTLRQAPDVILIGEIRTRETMEYAINFAETGHLVLSTLHANSA
NQALDRIINFFPEERRAQLLMDLSFNLKAIISQRLIRREAGGRVAAIEIMLNSPLIADLVFKGEIHELKAVMARSNEQGM
ITFDQFLFNLFEAGEISYEEALRNADSKNELRLRIKLDSKRAAQNLLDDEGVNSLLMKEEEIERRGLR

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=655880 JN531_RS09860 WP_275591450.1 2111163..2112329(+) (pilU) [Flagellatimonas centrodinii strain R2A-3]
ATGGAACGCGATCAAGCGGTCAAGATGATGCAGCAGCTGCTCAACCTGATGAAGGAGAAGGGCGGGTCCGATCTGTTCAT
CACCTCGGGGTTTCCGCCAGCGCTGAAGATCAATGGCCAGATGACACCGGTGGGCGACAAGGCGCTGCCGCCGGAAGCGG
CCGCGATCATGATGCGTGCCCTGATGAACGACCGTCAGACCAAGGAATTCGAAGCCACCAACGAGTGCAACTTCGCCATC
TCGCCCCCCAATGTCGGCCGTTTCCGTGTCAACGCCTTCGTTCAGCAGGGCCGGGTCGGCGGGGTGCTGCGCACCATCCA
GACCGAGATCCCCAGCTTCGAGCAATTGCGACTACCTGCCCGGTTGCAGGACATCGTGATGGAAAAGCGCGGCCTGGTGC
TGATGGTGGGAGGCACCGGCTCCGGCAAGTCGACCTCGTTGGCGGCCATGGTGGGGTATCGCAACCAGCAGAGCCGCGGG
CACATCATCACCATCGAGGACCCGGTGGAGTACGTGCACCCGCACGGCGGCTGCATCGTCACCCAGCGGGAGGTGGGGGC
CGACACCCAAAGCTGGGACAACGCCCTCAAGAACACCCTGCGACAGGCACCGGACGTGATCCTGATCGGCGAGATCCGCA
CCCGCGAGACCATGGAGTACGCGATCAACTTCGCCGAAACCGGGCATCTGGTGCTCTCCACCCTGCATGCCAACAGTGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCGCGCAGTTGCTGATGGACCTGTCGTTCAACCT
CAAGGCGATCATCTCGCAACGCCTGATCCGACGTGAGGCGGGGGGGCGGGTGGCAGCCATCGAGATCATGCTCAACTCGC
CGCTGATCGCCGATCTCGTCTTCAAGGGAGAGATTCACGAGTTGAAGGCGGTGATGGCGCGCTCGAACGAGCAGGGGATG
ATCACCTTCGATCAGTTCCTGTTCAACCTGTTCGAGGCCGGCGAGATCAGCTACGAAGAGGCCCTGCGCAATGCCGACAG
CAAGAATGAGCTGCGTCTGCGCATCAAGCTTGATTCCAAGCGTGCAGCGCAGAATCTGCTTGATGATGAAGGCGTCAACA
GCCTGCTGATGAAGGAAGAGGAAATCGAACGCAGGGGCCTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.615

92.268

0.559
  pilU Vibrio cholerae strain A1552

54.768

94.588

0.518
  pilU Acinetobacter baylyi ADP1

57.061

89.433

0.51
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.931

89.175

0.392
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.609

88.918

0.379
  pilT Vibrio cholerae strain A1552

42.609

88.918

0.379
  pilT Legionella pneumophila strain Lp02

41.176

87.629

0.361
  pilT Legionella pneumophila strain ERS1305867

41.176

87.629

0.361