Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JN531_RS09865 Genome accession   NZ_CP092104
Coordinates   2112329..2113390 (+) Length   353 a.a.
NCBI ID   WP_228348705.1    Uniprot ID   -
Organism   Flagellatimonas centrodinii strain R2A-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2107329..2118390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN531_RS09845 (JN531_009845) rpiA 2108628..2109290 (-) 663 WP_228348702.1 ribose-5-phosphate isomerase RpiA -
  JN531_RS09850 (JN531_009850) - 2109287..2110000 (-) 714 WP_228348703.1 YggS family pyridoxal phosphate-dependent enzyme -
  JN531_RS09855 (JN531_009855) pilT 2110100..2111134 (+) 1035 WP_228348704.1 type IV pilus twitching motility protein PilT Machinery gene
  JN531_RS09860 (JN531_009860) pilU 2111163..2112329 (+) 1167 WP_275591450.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JN531_RS09865 (JN531_009865) pilU 2112329..2113390 (+) 1062 WP_228348705.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JN531_RS09870 (JN531_009870) rplM 2113576..2114004 (+) 429 WP_228348706.1 50S ribosomal protein L13 -
  JN531_RS09875 (JN531_009875) rpsI 2114017..2114424 (+) 408 WP_228348707.1 30S ribosomal protein S9 -
  JN531_RS09885 (JN531_009885) - 2114621..2116702 (+) 2082 WP_228348708.1 S46 family peptidase -
  JN531_RS09890 (JN531_009890) - 2116668..2117651 (-) 984 WP_228348709.1 hypothetical protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39322.14 Da        Isoelectric Point: 6.7025

>NTDB_id=655881 JN531_RS09865 WP_228348705.1 2112329..2113390(+) (pilU) [Flagellatimonas centrodinii strain R2A-3]
MLKILPYLKLAVERNASDIFFTCNAPAMLKVDGDFLAIGKTTLTADFIRELAHNLLTPEQQQELRDECEIDLATQAGGLG
RFRVNIFTQRNQIGMVLRYVRADIPQLDDLGLPATLKDLVMLKRGLILMVGATGSGKSTSLAAMIHHRNLNAAGHILTIE
DPIEFVHGNHRSIVNQREVGTDTRSYERALKSSLREAPDVILIGEVRTRATMEATLTLANTGHLALSTLHANNAYQALQR
VINLYPDENRDQLYMDLSMTLRAVISQRLVRRKDGSRTAAVEVMINTPFIQDLILNRRIDDIREAMAQSSDKGMLSFDNS
LFALWKRGDIEVEEALANADSRANLEAKINFGG

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=655881 JN531_RS09865 WP_228348705.1 2112329..2113390(+) (pilU) [Flagellatimonas centrodinii strain R2A-3]
ATGCTGAAAATCCTGCCGTATCTGAAGCTGGCGGTGGAACGCAATGCCTCCGACATTTTCTTCACCTGCAATGCGCCGGC
GATGCTCAAGGTCGACGGTGATTTCCTGGCGATCGGCAAGACCACGCTCACGGCCGACTTCATCCGCGAACTCGCCCACA
ACCTGCTGACGCCGGAGCAACAGCAGGAGCTGCGCGACGAGTGCGAGATCGACCTCGCCACCCAGGCCGGTGGTCTCGGC
CGCTTCCGCGTCAATATCTTTACCCAGCGTAACCAGATCGGCATGGTGCTGCGTTATGTCAGGGCCGACATCCCCCAGCT
CGATGATCTCGGTCTGCCGGCAACGCTGAAGGATCTGGTGATGCTCAAGCGCGGGCTGATTCTAATGGTCGGCGCGACCG
GATCAGGCAAGTCGACCTCCCTGGCGGCGATGATCCACCATCGCAACCTCAACGCTGCGGGGCACATCCTCACCATTGAA
GACCCGATCGAGTTCGTCCACGGAAACCACCGCAGCATCGTCAACCAACGTGAGGTTGGCACCGACACTCGCAGTTACGA
ACGGGCATTGAAGAGCTCACTGCGCGAAGCGCCGGACGTAATCCTCATCGGCGAGGTGCGGACCCGCGCCACCATGGAGG
CCACGCTGACCCTTGCCAATACCGGGCACCTGGCACTGTCCACCCTGCATGCCAACAATGCCTACCAGGCGCTGCAGCGG
GTGATCAATCTGTATCCCGACGAGAACCGCGATCAGCTCTACATGGACCTGTCGATGACCCTGCGCGCGGTCATCAGTCA
GCGACTGGTGCGGCGCAAGGACGGCAGCCGCACCGCGGCTGTCGAGGTAATGATCAATACCCCGTTTATCCAGGACCTGA
TCCTCAACCGTCGCATTGACGATATCCGCGAGGCCATGGCGCAGTCCTCTGACAAGGGCATGCTGAGCTTCGATAACTCC
CTGTTTGCCCTGTGGAAGCGAGGCGATATCGAGGTGGAAGAGGCCCTGGCCAACGCCGATTCCCGCGCCAATCTCGAGGC
GAAGATCAATTTCGGTGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.578

98.017

0.496
  pilU Acinetobacter baylyi ADP1

48.563

98.584

0.479
  pilU Vibrio cholerae strain A1552

47.647

96.317

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.413

96.034

0.388
  pilT Acinetobacter baumannii D1279779

39.222

94.618

0.371
  pilT Acinetobacter nosocomialis M2

39.222

94.618

0.371
  pilT Acinetobacter baumannii strain A118

39.222

94.618

0.371
  pilT Pseudomonas aeruginosa PAK

37.791

97.45

0.368
  pilT Acinetobacter baylyi ADP1

38.235

96.317

0.368
  pilT Pseudomonas stutzeri DSM 10701

37.209

97.45

0.363