Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L1F30_RS01475 Genome accession   NZ_CP090959
Coordinates   318185..319219 (+) Length   344 a.a.
NCBI ID   WP_253358513.1    Uniprot ID   -
Organism   Simiduia sp. 21SJ11W-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 313185..324219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1F30_RS01455 (L1F30_01455) - 314765..315730 (+) 966 WP_253358505.1 calcium/sodium antiporter -
  L1F30_RS01460 (L1F30_01460) - 315888..316478 (-) 591 WP_253358507.1 YggT family protein -
  L1F30_RS01465 (L1F30_01465) proC 316482..317297 (-) 816 WP_253358509.1 pyrroline-5-carboxylate reductase -
  L1F30_RS01470 (L1F30_01470) - 317358..318047 (-) 690 WP_253358511.1 YggS family pyridoxal phosphate-dependent enzyme -
  L1F30_RS01475 (L1F30_01475) pilT 318185..319219 (+) 1035 WP_253358513.1 type IV pilus twitching motility protein PilT Machinery gene
  L1F30_RS01480 (L1F30_01480) pilU 319232..320374 (+) 1143 WP_253358515.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L1F30_RS01485 (L1F30_01485) ruvX 320388..320804 (-) 417 WP_253358517.1 Holliday junction resolvase RuvX -
  L1F30_RS01490 (L1F30_01490) - 320916..321509 (-) 594 WP_253358519.1 YqgE/AlgH family protein -
  L1F30_RS01495 (L1F30_01495) - 321560..322465 (-) 906 WP_253358521.1 energy transducer TonB -
  L1F30_RS01500 (L1F30_01500) gshB 322469..323437 (-) 969 WP_253358523.1 glutathione synthase -
  L1F30_RS01505 (L1F30_01505) pilG 323737..324132 (+) 396 WP_253358525.1 twitching motility response regulator PilG Regulator

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38233.95 Da        Isoelectric Point: 7.0158

>NTDB_id=646670 L1F30_RS01475 WP_253358513.1 318185..319219(+) (pilT) [Simiduia sp. 21SJ11W-1]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPPMQHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGQVFRDICNTPRGICLVTGPTGSGKSTTLAAMVDYINDNKYQHILTIED
PIEFVHESKKCLINQREVHRDTHGFSAALRSALREDPDIILVGEMRDLETIKLALEAAETGHLVFGTLHTQSAAKTIDRI
IDVFPAAEKAMVRSMLSESLQAVISQTLMKKNGGGRVAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGASTGMQTMDQCL
ADLVARRVINRDVAREKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=646670 L1F30_RS01475 WP_253358513.1 318185..319219(+) (pilT) [Simiduia sp. 21SJ11W-1]
ATGGATATTACGGAACTTCTCGCCTTTAGCGCAAAACAAGGCGCCTCAGACCTGCACCTTTCCTCCGGGCTGCCACCCAT
GATACGCGTAGACGGCGACGTGCGCCGTATTAATTTGCCGCCCATGCAACACAAGCAAGTGCACGGCTTGATCTATGAAA
TCATGAACGACAAGCAGCGCAAAGACTACGAAGAATTTCTGGAAACAGACTTCTCCTTTGAGGTGCCGGGCGTGGCGCGT
TTCCGTGTAAACGCCTTTAACCAAAACCGCGGCGCCGGCGCAGTTTTCCGTACCATTCCCTCGAAAGTGTTAACCATGGA
AGAGCTGGGCATGGGGCAGGTGTTTCGCGATATCTGCAATACCCCGCGCGGCATTTGCCTGGTAACCGGGCCTACGGGTT
CCGGTAAATCCACCACCCTTGCGGCCATGGTGGATTACATTAACGACAACAAATACCAGCACATCCTTACCATTGAAGAC
CCGATTGAATTTGTGCACGAAAGTAAAAAGTGCCTGATTAACCAGCGGGAAGTGCACCGCGACACCCACGGCTTCTCTGC
CGCATTGCGCTCGGCCTTGCGTGAAGATCCGGACATTATTCTGGTGGGTGAGATGCGCGACCTTGAAACCATCAAGCTCG
CCCTGGAAGCGGCCGAAACCGGCCACCTGGTGTTCGGTACCCTGCACACCCAGTCGGCGGCAAAAACCATCGACCGGATC
ATTGATGTATTCCCGGCCGCTGAAAAAGCCATGGTGCGCTCAATGCTGTCGGAATCATTGCAGGCGGTGATCTCGCAAAC
GCTGATGAAGAAAAACGGCGGTGGCCGGGTGGCAGCACACGAAATCATGCGCGGCACGCCCGCCATTCGTAACCTGATTC
GTGAAGATAAGGTGGCGCAAATGTACTCGGCGATTCAAACCGGCGCATCTACCGGCATGCAAACCATGGATCAATGCCTG
GCCGATCTCGTGGCGCGCCGCGTGATTAACCGCGACGTTGCCCGTGAAAAAGCAAAAATTCCAGAAAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

86.919

100

0.869
  pilT Pseudomonas stutzeri DSM 10701

86.628

100

0.866
  pilT Acinetobacter baumannii D1279779

83.43

100

0.834
  pilT Acinetobacter baumannii strain A118

83.43

100

0.834
  pilT Acinetobacter nosocomialis M2

83.14

100

0.831
  pilT Acinetobacter baylyi ADP1

80.523

100

0.805
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.118

98.837

0.683
  pilT Vibrio cholerae strain A1552

69.118

98.837

0.683
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39
  pilU Pseudomonas stutzeri DSM 10701

40.741

94.186

0.384
  pilU Vibrio cholerae strain A1552

40

94.477

0.378