Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L1F30_RS01480 Genome accession   NZ_CP090959
Coordinates   319232..320374 (+) Length   380 a.a.
NCBI ID   WP_253358515.1    Uniprot ID   -
Organism   Simiduia sp. 21SJ11W-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 314232..325374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1F30_RS01455 (L1F30_01455) - 314765..315730 (+) 966 WP_253358505.1 calcium/sodium antiporter -
  L1F30_RS01460 (L1F30_01460) - 315888..316478 (-) 591 WP_253358507.1 YggT family protein -
  L1F30_RS01465 (L1F30_01465) proC 316482..317297 (-) 816 WP_253358509.1 pyrroline-5-carboxylate reductase -
  L1F30_RS01470 (L1F30_01470) - 317358..318047 (-) 690 WP_253358511.1 YggS family pyridoxal phosphate-dependent enzyme -
  L1F30_RS01475 (L1F30_01475) pilT 318185..319219 (+) 1035 WP_253358513.1 type IV pilus twitching motility protein PilT Machinery gene
  L1F30_RS01480 (L1F30_01480) pilU 319232..320374 (+) 1143 WP_253358515.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L1F30_RS01485 (L1F30_01485) ruvX 320388..320804 (-) 417 WP_253358517.1 Holliday junction resolvase RuvX -
  L1F30_RS01490 (L1F30_01490) - 320916..321509 (-) 594 WP_253358519.1 YqgE/AlgH family protein -
  L1F30_RS01495 (L1F30_01495) - 321560..322465 (-) 906 WP_253358521.1 energy transducer TonB -
  L1F30_RS01500 (L1F30_01500) gshB 322469..323437 (-) 969 WP_253358523.1 glutathione synthase -
  L1F30_RS01505 (L1F30_01505) pilG 323737..324132 (+) 396 WP_253358525.1 twitching motility response regulator PilG Regulator
  L1F30_RS01510 (L1F30_01510) pilH 324194..324556 (+) 363 WP_253358527.1 twitching motility response regulator PilH -
  L1F30_RS01515 (L1F30_01515) - 324588..325142 (+) 555 WP_253358529.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42328.61 Da        Isoelectric Point: 6.2356

>NTDB_id=646671 L1F30_RS01480 WP_253358515.1 319232..320374(+) (pilU) [Simiduia sp. 21SJ11W-1]
MDFDRLLSLMVEKGASDLFITAGVPPSMKLHGKVVPVTTTPLSPEKSREVVLSVMNEKQRNEFLESKELNFAISARGIGR
FRCSAFYQRNLAGMVLRRIETNIPKIDDLGLPEIIKELAMTKRGLIIFVGATGTGKSTSLASMIGHRNRNSKGHIITIED
PIEFIHQHQGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRSRETMEHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHGQLWMDLALNLRGIIAQQLIPTPDGSGRRACLEVLLNTPLAQDLIRKGAVHELKPLMKKSTELGMQTFDQA
LYDLYDRGEITYEDALSYADSPNDLRLLIKLGSETDADYLSNAADELTIEKDESPSHKLY

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=646671 L1F30_RS01480 WP_253358515.1 319232..320374(+) (pilU) [Simiduia sp. 21SJ11W-1]
GTGGATTTTGATCGTTTATTGAGCCTGATGGTAGAGAAGGGCGCCTCGGATTTATTCATTACCGCCGGTGTGCCGCCATC
AATGAAATTGCACGGCAAGGTGGTGCCGGTAACCACCACGCCGCTCTCGCCAGAGAAATCCCGCGAGGTGGTGTTGTCTG
TGATGAATGAAAAGCAGCGCAACGAATTTTTGGAAAGCAAAGAATTAAACTTTGCTATTTCCGCGCGCGGTATTGGCCGT
TTCCGTTGCAGTGCGTTTTATCAGCGCAACCTGGCGGGCATGGTGTTGCGTCGCATTGAAACCAATATTCCGAAAATTGA
TGATTTGGGCCTGCCTGAAATTATCAAAGAGCTGGCCATGACCAAGCGCGGCCTGATTATTTTTGTGGGCGCCACCGGTA
CCGGTAAATCCACATCGCTCGCCTCCATGATTGGCCACCGCAATCGCAATTCCAAAGGCCACATCATTACCATTGAAGAC
CCCATTGAATTTATTCACCAGCACCAGGGTTGCATTGTGACCCAGCGCGAAGTGGGTATTGATACCGATTCATTTGAAGT
GGCGTTGAAAAACACCTTGCGGCAAGCCCCCGATGTGATTTTGATTGGTGAGGTGCGCTCGCGCGAAACCATGGAGCACG
CCATCGCCTTCGCTGAAACCGGCCACTTGTGTTTGTGCACCTTGCACGCCAACAACGCCAACCAGGCGCTCGACCGGATT
ATTCACTTCTTCCCGGCTGATCGTCACGGCCAGTTGTGGATGGACTTGGCCCTCAACTTGCGCGGCATTATTGCCCAGCA
GCTGATCCCAACGCCTGATGGCTCTGGCCGGCGCGCCTGTTTGGAGGTGTTGTTAAATACGCCGTTGGCGCAGGATTTGA
TTCGCAAGGGCGCGGTGCATGAATTAAAACCGCTCATGAAAAAATCCACCGAGCTTGGCATGCAAACCTTCGATCAGGCG
CTGTACGATTTGTACGACCGCGGCGAAATCACCTACGAAGATGCGCTCTCCTATGCGGATTCACCGAACGATTTGCGCCT
GCTGATCAAGCTGGGGTCGGAAACCGATGCCGACTACCTCTCCAACGCGGCCGACGAACTCACCATCGAGAAAGACGAGT
CGCCCAGCCACAAGCTTTACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

77.926

98.947

0.771
  pilU Acinetobacter baylyi ADP1

67.507

93.947

0.634
  pilU Vibrio cholerae strain A1552

53.514

97.368

0.521
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.253

91.579

0.405