Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KI615_RS20330 Genome accession   NZ_CP075186
Coordinates   4298947..4299990 (-) Length   347 a.a.
NCBI ID   WP_226417522.1    Uniprot ID   -
Organism   Dechloromonas denitrificans strain G6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4293947..4304990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI615_RS20305 (KI615_20250) - 4294273..4294509 (-) 237 WP_226471825.1 hypothetical protein -
  KI615_RS20310 (KI615_20255) - 4294649..4296640 (-) 1992 WP_226417519.1 UvrD-helicase domain-containing protein -
  KI615_RS20315 (KI615_20260) - 4296710..4297027 (-) 318 WP_226471826.1 hypothetical protein -
  KI615_RS20320 (KI615_20265) - 4297078..4297764 (-) 687 WP_226471827.1 DUF4197 domain-containing protein -
  KI615_RS20325 (KI615_20270) pilU 4297777..4298913 (-) 1137 WP_319002314.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI615_RS20330 (KI615_20275) pilT 4298947..4299990 (-) 1044 WP_226417522.1 type IV pilus twitching motility protein PilT Machinery gene
  KI615_RS20335 (KI615_20280) - 4300047..4300736 (+) 690 WP_226471828.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI615_RS20340 (KI615_20285) proC 4300733..4301545 (+) 813 WP_226471829.1 pyrroline-5-carboxylate reductase -
  KI615_RS20345 (KI615_20290) - 4301545..4302078 (+) 534 WP_226471830.1 YggT family protein -
  KI615_RS20350 (KI615_20295) - 4302075..4302368 (+) 294 WP_226471831.1 DUF167 domain-containing protein -
  KI615_RS20355 (KI615_20300) comE 4302390..4302680 (-) 291 WP_226471832.1 ComEA family DNA-binding protein Machinery gene
  KI615_RS20360 (KI615_20305) - 4302807..4304054 (-) 1248 WP_226471833.1 dihydroorotase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38385.01 Da        Isoelectric Point: 6.8848

>NTDB_id=568468 KI615_RS20330 WP_226417522.1 4298947..4299990(-) (pilT) [Dechloromonas denitrificans strain G6]
MDITELLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINLPAMEHKDVHGMVYDIMNDGQRKVYEETLECDFSFEIPNLAR
FRVNAFNQQRGAGAVFRTIPSKVLTLEELNCPKIFKDISEYPRGIVLVTGPTGSGKSTTLAAMVNHVNENEYGHILTVED
PIEFVHEAKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGQNVGMQTLDQN
LLDMVRRNVVSSAEARNKAANKDAFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=568468 KI615_RS20330 WP_226417522.1 4298947..4299990(-) (pilT) [Dechloromonas denitrificans strain G6]
ATGGACATCACCGAACTGCTGGCCTTCAGCGTCAAGAACAAGGCCTCCGACCTCCATCTTTCGAGCGGATTGCCGCCGAT
GATCCGCGTGCATGGCGACGTGCGCCGCATCAATCTGCCGGCGATGGAGCACAAGGATGTGCACGGCATGGTCTATGACA
TCATGAACGACGGCCAGCGCAAGGTGTACGAAGAAACGCTGGAGTGCGACTTTTCCTTCGAAATCCCCAATCTGGCGCGT
TTTCGGGTCAATGCCTTCAATCAGCAACGGGGCGCCGGCGCGGTCTTCCGGACCATTCCATCCAAGGTGCTGACCCTGGA
AGAGCTCAACTGCCCGAAGATTTTCAAGGATATTTCCGAGTACCCGCGCGGTATCGTGCTGGTCACCGGGCCGACCGGTT
CCGGCAAATCGACGACGCTGGCCGCCATGGTCAATCATGTCAATGAAAACGAATACGGTCATATCCTGACCGTCGAAGAT
CCGATTGAATTCGTCCATGAAGCCAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGACCGCACACGCTGTCCTTCTCGAA
CGCCTTGCGCTCGGCGCTGCGCGAGGATCCGGACGTCATCCTGGTCGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCTGAAACCGGCCACCTGGTGTTCGGCACGCTGCATACCTCGTCGGCGGCCAAGACCGTCGACCGTATC
GTCGACGTCTTCCCGGCGGCGGAAAAGGAAATGGTCCGTTCGATGTTGTCCGAGTCGCTGCGTGCCGTCATCTCGCAAAC
GCTGTTGAAGACCAAGGATGGGCAGGGCCGGGTTGCGGCGCACGAGATCATGATCGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAAAACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCCAGAACGTCGGCATGCAGACGCTCGATCAGAAT
CTGCTGGACATGGTTCGCCGCAATGTCGTATCGAGTGCCGAAGCGCGTAACAAGGCAGCCAACAAGGATGCTTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.13

99.424

0.787
  pilT Acinetobacter baumannii strain A118

79.13

99.424

0.787
  pilT Acinetobacter baumannii D1279779

79.13

99.424

0.787
  pilT Acinetobacter baylyi ADP1

76.232

99.424

0.758
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Neisseria meningitidis 8013

72.174

99.424

0.718
  pilT Neisseria gonorrhoeae MS11

71.884

99.424

0.715
  pilT Legionella pneumophila strain ERS1305867

71.594

99.424

0.712
  pilT Legionella pneumophila strain Lp02

71.594

99.424

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.822

97.406

0.68
  pilT Vibrio cholerae strain A1552

69.822

97.406

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

41.298

97.695

0.403
  pilU Acinetobacter baylyi ADP1

41.176

97.983

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.003

97.695

0.401