Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KI615_RS20325 Genome accession   NZ_CP075186
Coordinates   4297777..4298913 (-) Length   378 a.a.
NCBI ID   WP_319002314.1    Uniprot ID   -
Organism   Dechloromonas denitrificans strain G6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4292777..4303913
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI615_RS20295 (KI615_20240) - 4293077..4293259 (+) 183 WP_226417516.1 hypothetical protein -
  KI615_RS20300 (KI615_20245) - 4293694..4294143 (+) 450 WP_226471824.1 c-type cytochrome -
  KI615_RS20305 (KI615_20250) - 4294273..4294509 (-) 237 WP_226471825.1 hypothetical protein -
  KI615_RS20310 (KI615_20255) - 4294649..4296640 (-) 1992 WP_226417519.1 UvrD-helicase domain-containing protein -
  KI615_RS20315 (KI615_20260) - 4296710..4297027 (-) 318 WP_226471826.1 hypothetical protein -
  KI615_RS20320 (KI615_20265) - 4297078..4297764 (-) 687 WP_226471827.1 DUF4197 domain-containing protein -
  KI615_RS20325 (KI615_20270) pilU 4297777..4298913 (-) 1137 WP_319002314.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI615_RS20330 (KI615_20275) pilT 4298947..4299990 (-) 1044 WP_226417522.1 type IV pilus twitching motility protein PilT Machinery gene
  KI615_RS20335 (KI615_20280) - 4300047..4300736 (+) 690 WP_226471828.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI615_RS20340 (KI615_20285) proC 4300733..4301545 (+) 813 WP_226471829.1 pyrroline-5-carboxylate reductase -
  KI615_RS20345 (KI615_20290) - 4301545..4302078 (+) 534 WP_226471830.1 YggT family protein -
  KI615_RS20350 (KI615_20295) - 4302075..4302368 (+) 294 WP_226471831.1 DUF167 domain-containing protein -
  KI615_RS20355 (KI615_20300) comE 4302390..4302680 (-) 291 WP_226471832.1 ComEA family DNA-binding protein Machinery gene

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42193.59 Da        Isoelectric Point: 6.5061

>NTDB_id=568467 KI615_RS20325 WP_319002314.1 4297777..4298913(-) (pilU) [Dechloromonas denitrificans strain G6]
MERDQAMKFMHDLLRLMSQKKGSDLFITAGFPPAIKIDGKITPVSNQVLTSSHTAELARSIMNDRQAAEFEATKECNFAI
SPAGIGRFRVNALVQQGRVGVVCRTINMTIPTLDELQLPPVLKDLAMTKRGLIIFVGGTGTGKTTSLAALVDYRNENTYG
HIITIEDPIEYVHEHKNCIVTQREVGVDTDDWGPALKNTLRQAPDVILMGEIRDRDTMDYAIAFAETGHLALATLHANSA
NQAIDRIINFFPEERRQQLLMDLSLNLRALVSQRLIPKKDGKGRMAAIEVMLNSPLISDLIFKGEVHEIKEIMKKSRELG
MQTFDQALFDLYEAGKITYEDALRNADSLNDLRLQIKLHGKESKDRDLSTGIGHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=568467 KI615_RS20325 WP_319002314.1 4297777..4298913(-) (pilU) [Dechloromonas denitrificans strain G6]
ATGGAACGCGATCAGGCAATGAAATTCATGCACGATCTTTTGCGCCTGATGTCGCAAAAAAAAGGATCGGACCTTTTCAT
CACTGCCGGCTTCCCGCCGGCGATCAAGATAGACGGCAAGATCACGCCGGTATCGAATCAGGTGCTGACTTCCTCGCACA
CCGCCGAACTCGCCCGTTCGATCATGAACGACCGGCAGGCCGCCGAGTTCGAGGCGACCAAGGAATGCAACTTCGCCATC
TCGCCGGCCGGCATCGGCCGTTTCCGGGTCAATGCGCTGGTTCAGCAGGGCCGCGTCGGCGTCGTCTGCCGAACCATCAA
CATGACCATCCCGACGCTCGACGAACTGCAATTGCCACCGGTCCTCAAGGACCTGGCGATGACCAAGCGCGGCCTGATCA
TCTTCGTCGGCGGTACCGGTACCGGCAAGACGACCTCGCTGGCGGCACTGGTCGATTACCGCAATGAAAATACCTACGGC
CACATCATCACGATCGAGGATCCGATCGAGTATGTGCACGAACACAAGAACTGCATCGTCACGCAGCGCGAAGTCGGTGT
CGATACCGACGACTGGGGACCGGCGCTGAAGAACACGCTGCGTCAGGCGCCGGACGTCATCCTGATGGGCGAAATCCGCG
ACCGCGATACGATGGACTACGCCATCGCCTTCGCCGAAACCGGCCACCTGGCGCTGGCTACGCTGCACGCCAACAGTGCC
AACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAGCGCCGTCAGCAACTGCTGATGGACCTTTCGCTGAACCT
GCGTGCCCTGGTGTCGCAGCGCCTGATCCCGAAAAAGGACGGCAAGGGCCGGATGGCGGCCATTGAAGTGATGCTCAACT
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGAAGTCCATGAGATCAAGGAAATCATGAAGAAGTCCCGCGAACTCGGC
ATGCAGACTTTCGACCAGGCGCTGTTCGACCTCTACGAGGCGGGCAAGATCACCTATGAAGATGCGCTGCGCAATGCCGA
TTCGCTGAACGACCTGCGCCTGCAGATCAAGCTGCACGGCAAGGAATCCAAGGACCGTGACCTGAGCACCGGGATCGGTC
ACCTCGATATTGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.736

96.296

0.614
  pilU Acinetobacter baylyi ADP1

60.446

94.974

0.574
  pilU Vibrio cholerae strain A1552

54.545

90.212

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.881

88.624

0.389
  pilT Pseudomonas aeruginosa PAK

42.687

88.624

0.378
  pilT Acinetobacter baylyi ADP1

42.136

89.153

0.376
  pilT Legionella pneumophila strain Lp02

42.296

87.566

0.37
  pilT Legionella pneumophila strain ERS1305867

42.296

87.566

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.194

88.624

0.365
  pilT Acinetobacter nosocomialis M2

41.39

87.566

0.362
  pilT Acinetobacter baumannii D1279779

41.39

87.566

0.362
  pilT Acinetobacter baumannii strain A118

41.39

87.566

0.362